Results 211 to 220 of about 54,220 (246)

Regulation of NHE3 by nitric oxide in Caco-2 cells

American Journal of Physiology-Gastrointestinal and Liver Physiology, 2002
The effect of nitric oxide (NO) on Na+/H+exchange (NHE) activity was investigated utilizing Caco-2 cells as an experimental model. Incubation of Caco-2 cells with 10−3M S-nitroso- N-acetylpenicillamine (SNAP), a conventional donor of NO, for 20 min resulted in a ∼45% dose-dependent decrease in NHE activity, as determined by assay of ...
Ravinder K, Gill, Seema, Saksena, Irfan Ali, Syed, Sangeeta, Tyagi, Waddah A, Alrefai, Jaleh, Malakooti, Krishnamurthy, Ramaswamy, Pradeep K, Dudeja   +7 more
openaire   +2 more sources

Osmotic stress, a proinflammatory signal in Caco-2 cells

Biochimie, 2004
Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory cytokines and the stimulatory ...
Aurélie, Hubert, Bruno, Cauliez, Arlette, Chedeville, Annie, Husson, Alain, Lavoinne   +4 more
openaire   +2 more sources

Stereoselectivity of the reduced folate carrier in Caco‐2 cells

Chirality, 2005
AbstractStereoselectivity of the human reduced folate carrier (RFC1) was examined in Caco‐2 cells using methotrexate (l‐amethopterin or l‐MTX) and its antipode (d‐amethopterin or d‐MTX) as model substrates. The initial uptake rate of folic acid (FA) was concentration dependent, with a Km value of approximately 0.6 μM. The Eadie–Hofstee plot of the RFC1‐
Tomoya, Narawa, Rikako, Shimizu, Shuhei, Takano, Yasuyuki, Tsuda, Kiyoko, Ono, Hideo, Yamada, Tomoo, Itoh   +6 more
openaire   +2 more sources

Determination of Iron in Caco-2 cells by ET-AAS

Analytical and Bioanalytical Chemistry, 2005
An electrothermal atomic absorption spectrometric method (ET-AAS) was developed for the direct determination of iron in intestinal Caco-2 cells after studying cell viability and proliferation using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT test).
Zeiner, Michaela, Zödl, Bettina, Steffan, Ilse, Marktl, Wolfgang, Ekmekcioglu, Cem   +4 more
openaire   +2 more sources

Campylobacter jejuni motility and invasion of Caco-2 cells

Infection and Immunity, 1995
We investigated the influence of motility on Campylobacter jejuni binding and invasion of Caco-2 cells. C. jejuni was motile in soft agar at basic (pH 8.5) and neutral pH values representative of the intestinal environment. However, C. jejuni was immobilized at pH 5.0. The inability of C.
C M, Szymanski, M, King, M, Haardt, G D, Armstrong   +3 more
openaire   +2 more sources

Toxic and biochemical effects of zinc in Caco-2 cells

Journal of Inorganic Biochemistry, 2003
Zinc (in relatively high concentrations) can be toxic to intestinal cells. The aim of the present study was to quanitfy cellular injury in preconfluent, colonic cancerous cells and in postconfluent, differentiating human intestinal Caco-2 cells. Cellular damage was measured by using cell proliferation, lactate dehydrogenase (LDH)-release, and apoptosis
Zödl, Bettina, Zeiner, Michaela, Sargazi, Mansour, Roberts, Norman Bryson, Marktl, Wolfgang, Steffan, Ilse, Ekmekcioglu, Cem   +6 more
openaire   +3 more sources

Putrescine transport in differentiated Caco-2 cells.

Gastroenterologie clinique et biologique, 1996
To evaluate the putative importance of digestive luminal polyamines, we investigated the role of oxidation in putrescine uptake and transport in differentiated Caco-2 cells grown in monolayer.Our results confirm a putrescine uptake system located both at the apical and basolateral cell membrane. Putrescine is efficiently transported and excreted in the
Barlier, A.M., Benamouzig, Robert, Tome, D.   +2 more
openaire   +2 more sources

Novel Choline Transport Characteristics in Caco-2 Cells

Drug Development and Industrial Pharmacy, 2002
Choline transport is characterized by sodium-dependent high-affinity, sodium-independent low-affinity, and sodium-independent blood-brain barrier transport mechanisms. Each defined mechanism has specific characteristics with regard to affinity for choline, transport capacity, and inhibition by hemicholinium.
A P, Crowe, P R, Lockman, T J, Abbruscato, D D, Allen   +3 more
openaire   +2 more sources

Competition Effects on Carotenoid Absorption by Caco-2 Cells

2009
International ...
Reboul, Emmanuelle, Borel, Patrick
openaire   +3 more sources

Transepithelial Taurine Transport in Caco-2 Cell Monolayers

Journal of Membrane Biology, 2005
Here we characterized transepithelial taurine transport in monolayers of cultured human intestinal Caco-2 cells by analyzing kinetic apical and basolateral uptake and efflux parameters. Basolateral uptake was Na(+)- and Cl(-)- dependent and was inhibited by beta-amino acids.
S, Roig-Pérez, M, Moretó, R, Ferrer
openaire   +2 more sources