Results 171 to 180 of about 6,761 (193)
Crystal structure and functional properties of the human CCR4-CAF1 deadenylase complex [PDF]
Nucleic Acids Research, 2021 Abstract The CCR4 and CAF1 deadenylases physically interact to form the CCR4-CAF1 complex and function as the catalytic core of the larger CCR4-NOT complex. Together, they are responsible for the eventual removal of the 3′-poly(A) tail from essentially all cellular mRNAs and consequently play a central role in the posttranscriptional ...
Ying Chen +2 more
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Tuneable hydrogels of Caf1 protein fibers
Materials Science and Engineering C, 2018Capsular antigen fraction 1 (Caf1) is a robust polymeric protein forming a protective layer around the bacterium Yersinia pestis. Occurring as ≈1 μm polymeric fibers, it shares its immunoglobulin-like fold with the majority of mammalian extracellular proteins such as fibronectin and this structural similarity suggests that this unusual polymer could ...
Gema Dura +2 more
exaly +4 more sources
The N-terminus modulates human Caf1 activity, structural stability and aggregation
International Journal of Biological Macromolecules, 2012Caf1 is a deadenylase component of the CCR4-Not complex. Here we found that the removal of the N-terminus resulted in a 30% decrease in human Caf1 (hCaf1) activity, but had no significant influence on main domain structure. The removal of the N-terminus led to a decrease in the thermal stability, while the existence of the N-terminus promoted hCaf1 ...
Yong-Bin Yan
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Mammalian miRNA RISC Recruits CAF1 and PABP to Affect PABP-Dependent Deadenylation [PDF]
Molecular Cell, 2009 MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract.
Marc R Fabian +2 more
exaly +5 more sources
Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin [PDF]
Nucleic Acids Research, 2011 The carbon catabolite repressor protein 4 (Ccr4)-Negative on TATA (Not) complex controls gene expression at two levels. In the nucleus, it regulates the basal transcription machinery, nuclear receptor-mediated transcription and histone modifications. In the cytoplasm, the complex is required for messenger RNA (mRNA) turnover through its two associated ...
Georg Stoecklin
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The structure of Yersinia pestis Caf1 polymer in free and adjuvant bound states
Vaccine, 2010Caf1 of the plague bacterium, Yersinia pestis is a polymeric virulence factor and vaccine component, formed from monomers by a donor strand exchange (DSE) mechanism. Here, EM images of Caf1 reveal flexible polymers up to 1.5 microm long (4MDa). The bead-like structures along the polymer are 5.8 + or - 1 nm long and correspond to single Caf1 proteins ...
Jeremy H Lakey
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Mouse CAF1 Can Function As a Processive Deadenylase/3′–5′-Exonuclease in Vitro but in Yeast the Deadenylase Function of CAF1 Is Not Required for mRNA Poly(A) Removal [PDF]
Journal of Biological Chemistry, 2004 The mouse CAF1 (mCAF1) is an ortholog of the yeast (y) CAF1 protein, which is a component of the CCR4-NOT complex, the major cytoplasmic deadenylase of Saccharomyces cerevisiae. Although CAF1 protein belongs to the DEDDh family of RNases, CCR4 appears to be the principle deadenylase of the CCR4-NOT complex.
Clyde L Denis
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Conformational states and association mechanism of Yersinia pestis Caf1 subunits
Biochemical and Biophysical Research Communications, 2008Bacterial infectivity often relies on efficient attachment to the host cells through adhesive extensions. Unveiling the structural basis of the formation of these organelles is of paramount importance for both academic and applicative implications.
Vitagliano Luigi +3 more
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The HP1α–CAF1–SetDB1‐containing complex provides H3K9me1 for Suv39‐mediated K9me3 in pericentric heterochromatin [PDF]
EMBO Reports, 2009 Trimethylation of lysine 9 in histone H3 (H3K9me3) enrichment is a characteristic of pericentric heterochromatin. The hypothesis of a stepwise mechanism to establish and maintain this mark during DNA replication suggests that newly synthesized histone H3 goes through an intermediate methylation state to become a substrate for the histone ...
Alejandra Loyola +2 more
exaly +3 more sources