Results 131 to 140 of about 407,718 (365)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

, 2023
Xianqin Song,1 Yu Song,1 Quanping Ma,2 Kehua Fang,3 Xiaotian Chang1 1Medical Research Center of the Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 2Clinical Laboratory, The fourth People’s Hospital of Jinan ...
Ma Q, Fang K, Song X, Chang X, Song Y
core  

Ultra-short echo time cardiovascular magnetic resonance of atherosclerotic carotid plaque [PDF]

, 2010
Background Multi-contrast weighted cardiovascular magnetic resonance (CMR) allows detailed plaque characterisation and assessment of plaque vulnerability.
Sonia Nielles-Vallespin, Nielles-Vallespin, S, Firmin, DN, Chan, Cheuk F, Keenan, Niall G, Mary N Sheppard, Gatehouse, Peter, Chan Cheuk F, Firmin David N, Gatehouse, P, Keenan, NG, Sheppard, MN, Boyle, JJ, Peter Gatehouse, Chan, CF, Sheppard Mary N, Boyle Joseph J, Joseph J Boyle, Niall G Keenan, Nielles-Vallespin, Sonia, Boyle, Joseph J, Sheppard, Mary N, Pennell, Dudley J, Pennell, DJ, Pennell Dudley J, Gatehouse Peter, Dudley J Pennell, Nielles-Vallespin Sonia, David N Firmin, Cheuk F Chan, Keenan Niall G, Firmin, David N   +31 more
core   +1 more source

Adventitial MSC-like cells are progenitors of vascular smooth muscle cells and drive vascular calcification in chronic kidney disease

Cell Stem Cell, 2016
Mesenchymal stem cell-like (MSC-like) cells reside in the vascular wall but their role in vascular regeneration and disease is poorly understood. Here, we show that Gli1+ cells located in the arterial adventitia are progenitors of vascular smooth muscle ...
R. Kramann, C. Goettsch, J. Wongboonsin, H. Iwata, R. Schneider, C. Kuppe, Nadine Kaesler, Monica Chang-Panesso, Flavia G Machado, Susannah Gratwohl, K. Madhurima, Joshua D. Hutcheson, Sanjay Jain, E. Aikawa, B. Humphreys   +14 more
semanticscholar   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Diagnosis and Management Strategies in Sclerochoroidal Calcification: A Systematic Review

, 2023
Ahmet Kaan Gündüz,1,2,* Diğdem Tetik1,* 1Department of Ophthalmology, Ankara University Faculty of Medicine, Ankara, Turkey; 2Private Eye Clinic, Farilya Business Center 8/38, Ankara, 06510, Turkey*These authors contributed equally to ...
Tetik D, Gündüz AK
core  

Extracellular Vesicles As Mediators of Cardiovascular Calcification

Frontiers in Cardiovascular Medicine, 2017
Involvement of cell-derived extracellular particles, coined as matrix vesicles (MVs), in biological bone formation was introduced by Bonucci and Anderson in mid-1960s. In 1983, Anderson et al.
Amirala Bakhshian Nik, Joshua D. Hutcheson, E. Aikawa   +2 more
semanticscholar   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

Scientific Reports, 2018
Magnesium has been shown to effectively prevent vascular calcification associated with chronic kidney disease. Magnesium has been hypothesized to prevent the upregulation of osteoblastic genes that potentially drives calcification. However, extracellular
Anique D. ter Braake, P. Tinnemans, C. Shanahan, J. Hoenderop, J. D. de Baaij   +4 more
semanticscholar   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source