Results 171 to 180 of about 652,107 (224)

A Lambda Red Recombinase‐Assisted CRISPR/Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciens

Plant Biotechnology Journal, Volume 24, Issue 11, Page 6010-6027, November 2026.
ABSTRACT Agrobacterium tumefaciens is a powerful delivery tool for plant genetic transformation and a pivotal platform for agricultural biotechnology. However, laboratory strains of Agrobacterium have remained genetically unoptimized for decades, with genome engineering hindered by inefficient, endogenous homologous recombination systems.
Minmin Zheng, Rui Liu, Wei‐Chang Huang, Zuren Li, Kai Hua   +4 more
wiley   +1 more source

Dynamic Operations in Macromolecular Data Storage

Angewandte Chemie, Volume 138, Issue 44, 26 October 2026.
With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski, Oskar Affenberger, Jean‐François Lutz, Maria Nerantzaki   +3 more
wiley   +2 more sources

Calcium electroporation and electrochemotherapy for cancer treatment: Importance of cell membrane composition investigated by lipidomics, calorimetry and in vitro efficacy. [PDF]

Sci Rep, 2019
Hoejholt KL, Mužić T, Jensen SD, Dalgaard LT, Bilgin M, Nylandsted J, Heimburg T, Frandsen SK, Gehl J.   +8 more
europepmc   +1 more source

Single‐cell transcriptome atlas reveals a WRKY33‐HSP81 regulatory module that orchestrates phenylpropanoid‐mediated defense against Sclerotium rolfsii in Dendrobium officinale

The Plant Journal, Volume 128, Issue 3, November 2026.
SUMMARY Plants employ cell‐type‐specific transcriptional programs to defend against pathogens, yet the cellular heterogeneity of immune responses in the medicinal orchid Dendrobium officinale Kimura et Migo remains largely unexplored. A single‐cell transcriptomic atlas of the D.
Lingli Li, Ye Xu, Wei Liu, Zhitao Niu, Qingyun Xue, Xiaoyu Ding   +5 more
wiley   +1 more source

Natural variation in the evolutionarily conserved F‐box gene SDEF promotes seed dormancy in rice

The Plant Journal, Volume 128, Issue 3, November 2026.
SUMMARY Insufficient seed dormancy causes pre‐harvest sprouting (PHS) and severe yield losses in rice; however, genetic resources with practical breeding value remain limited. Here, we isolated a major dormancy gene, SDEF, via map‐based cloning using chromosomal single segment substitution lines (SSSLs), identifying a functional SNP (Arg‐to‐Trp) as the
Yuliang Zhou, Yueping Wang, Guangmiao Zhao, Chunbao Liu, Ruizhen Zeng, Wenxuan Liang, Ru Han, Hui Kong, Xiaofan Cao, Minshi Li, Huhui Chen, Bin Tan, Hong Liu, Zhenjiang Xu   +13 more
wiley   +1 more source

Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

The FASEB Journal, Volume 40, Issue 20, 31 October 2026.
Our previous study demonstrated that elevated levels of desmin and vimentin reduce BSM contractility. This study shows that the reduced BSM contractility is associated with mitochondrial impairment. G0/G1 switch gene 2 (G0S2) is upregulated in desmin‐ and vimentin‐overexpressing BSM.
Elham Javed, Chellappagounder Thangavel, Karthik Thangavel, Joseph Hypolite, Isabelle Gamache, Yilin Wang, Sankar Addya, Darin Mak, Furqan Syed, Jyotsna Mishra, Vilas Desai, Pragnya Das, Dominic R. Villalba, Alok Bhushan, Shey‐Shing Sheu, Sunday A. Shoyele, Erin L. Seifert, Jose G. Teodoro, Steven S. An, Raymond B. Penn, Deepak A. Deshpande, Ettickan Boopathi   +21 more
wiley   +1 more source

Ets2 Is an Activity‐Dependent Transcriptional Regulator of a Fast‐Twitch Muscle Program

The FASEB Journal, Volume 40, Issue 20, 31 October 2026.
Ets2 is a transcription factor rapidly (24 h) upregulated in skeletal muscle by fast 150 Hz, but not slow 20 Hz, stimulation patterns. Ets2 selectively translocates to nuclei of slow soleus muscle when stimulated by fast‐patterned, but not slow‐patterned, activity. Ets2 binds to a consensus DNA sequence in the troponin I fast intronic enhancer (TnIFIRE)
Zaheer A. Rana, Jo C. Bruusgaard, Jens R. Wendland, Ivan Winje, Ingrid M. Egner, Irina Karavanova, Einar Eftestøl, Detlef Vullhorst, Kristian Gundersen, Andres Buonanno   +9 more
wiley   +1 more source

ESOPE-Equivalent Pulsing Protocols for Calcium Electroporation: An In Vitro Optimization Study on 2 Cancer Cell Models. [PDF]

Technol Cancer Res Treat, 2018
Romeo S, Sannino A, Scarfì MR, Vernier PT, Cadossi R, Gehl J, Zeni O.   +6 more
europepmc   +1 more source

Design Considerations for Polymer–Deep Eutectic Solvent Hybrid Systems for Transdermal Drug Delivery

Journal of Applied Polymer Science, Volume 143, Issue 40, October 20, 2026.
Deep eutectic solvents (DESs) act as tunable, green excipients that modulate polymer structure–property relationships to enhance drug solubility, skin permeation, stability, and controlled release. Through hydrogen‐bond interactions, DES–polymer hybrid systems enable improved transdermal performance, offering a rational, design‐driven platform for ...
Madhavi Kailas Kapale, Madhur Kulkarni, Pranav J. Shah, Furqan A. Maulvi   +3 more
wiley   +1 more source

Electroporation induced protein elution out to extracellular media and cytoplasmic membrane blebbing

Electroporation, a method that uses electrical pulses to temporarily permeabilize cell membranes, has emerged as a useful tool in cell biology and biotechnology.
Jakštys, Baltramiejus, Vykertas, Salvijus, Šatkauskas, Saulius   +2 more
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