Quantification of calpastatin using an optical surface plasmon resonance biosensor
An immunological biosensor for calpastatin was developed on a surface plasmon resonance based system (Biacore Q). The performance of the biosensor assay was evaluated using ovine and bovine muscle and heart extracts with known calpastatin activity.
van der Palen, JGP +5 more
core +1 more source
Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli
A member of the attaching and effacing (AE) family of pathogens, enterohemorrhagic Escherichia coli (EHEC) induces dramatic changes to the intestinal cell cytoskeleton, including effacement of microvilli.
YuShuan eLai +5 more
doaj +1 more source
Fecal extracellular vesicles (fEVs) were isolated from preserved stool samples in DNA/RNA shield and characterized. In an in vitro model with PBMC, fEVs of pregnant women modulate T cells toward a regulatory phenotype favorable for pregnancy compared to fEVs of non‐pregnant women. ABSTRACT Pregnancy requires immune tolerance to a semi‐allogeneic fetus,
Stefanie Dietz‐Ziegler +17 more
wiley +1 more source
Quantification of Calpastatin Using an Optical Surface Plasmon Resonance Biosensor [PDF]
An immunological biosensor for calpastatin was developed on a surface plasmon resonance based system (Biacore Q). The performance of the biosensor assay was evaluated using ovine and bovine muscle and heart extracts with known calpastatin activity.
Kent, M. P. +5 more
core
Modulation of rat brain calpastatin efficiency by post-translational modifications [PDF]
Calpains, the thiol proteinases of the calcium-dependent proteolytic system, are regulated by a natural inhibitor, calpastatin, which is present in brain tissue in two forms. Although both calpastatins are highly active on human erythrocyte calpain, only
Sandro Pontremoli +11 more
core +1 more source
Analysis of Population Structure and Selection Signatures in Two Iraqi Fat‐Tailed Sheep Breeds
Awassi sheep exhibit clear genetic separation, whereas Hamdani sheep show an admixed profile, with iHS, ROH and Tajima's D analyses identifying breed‐specific candidate genes. Enrichment in immune response, hormone regulation and cellular signalling pathways highlights adaptive evolution and guides future breeding and conservation.
Mervan Bayraktar +6 more
wiley +1 more source
Identification of active calpastatin molecular species.
A) Activity profiles of samples from 106 erythroid precursor cells isolated from control and patients with mild and severe clinical symptoms, respectively. Numbers at the bottom refer to the molecular weight of proteins present in the gel slices.
Tipparat Penglong (164413) +8 more
core +1 more source
New allelic variant of the ovine calpastatin gene [PDF]
Calpastatin, a specific inhibitor of the calpains, participates in muscle growth and tenderization. Amplified fragment of 12 intron of CAST was used; the gene encoding calpastatin, from 164 lambs from synthetic lines for restriction fragment length ...
Greguła-Kania, M
core +1 more source
Short communication: Analysis of polymorphisms in candidate’s genes for meat quality in Lidia cattle
The aim of this paper was to analyze the segregation of some polymorphisms in three genes (CAPN1, CAST and DGAT1) related to meat quality in the Lidia cattle breed and some of its main lineages.
Rocio Pelayo +3 more
doaj +1 more source
Abstract 4755: Calpastatin phosphorylation regulates radiation response in glioblastoma
Glioblastoma (GBM) is the most prevalent and aggressive primary malignant brain tumor in adults. Patients diagnosed with GBM have a very poor prognosis and quality of life, with a median survival time of 12-15 months despite receiving the standard of ...
Mitchell Pearson +4 more
core +1 more source

