Results 101 to 110 of about 71,965 (172)

Construction of Reporter Phage T4::Nluc and Its Application in the Detection of Escherichia coli in Urinary Tract Infections

iLABMED, EarlyView.
A reporter phage T4::Nluc was constructed using the CRISPR/Cas9 system combined with homologous recombination and was applied for the detection of clinical Escherichia coli in urinary tract infections (UTIs). T4::Nluc exhibited superior sensitivity and specificity, and had a short detection time, making it a powerful tool for diagnosing UTIs.
Zhiyun Hao, Minwei Li, Qiang Zhao, Liye Wang, Ting Liu, Chi Wang, Chengbin Wang   +6 more
wiley   +1 more source

Generative artificial intelligence in medical imaging: Current landscape, challenges, and future directions

Interdisciplinary Medicine, EarlyView.
This review explores the applications of Generative AI (GAI) in medical imaging, with emphasis on its potential to enhance AI training and personalized medicine. The study comprehensively examines frameworks for evaluating the validity of GAI‐generated images while identifying critical challenges including model bias, data augmentation reliability, and
Wenle He, Xuewei Wu, Zhe Jin, Jie Sun, Xiaofeng Jiang, Shuixing Zhang, Bin Zhang   +6 more
wiley   +1 more source

Multi‐cycle high‐throughput growth media optimization using batch Bayesian optimization

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND The optimization of growth media is a crucial step in enhancing microbial growth and product formation in industrial bioprocesses. While Design of Experiments (DoE) is widely used for this purpose, it has inherent limitations, particularly in capturing complex interactions between media components and managing the high number of ...
Frédéric M. Lapierre, Pasquale Mattaliano, Dennis Raith, Mariela Castillo‐Cota, Jonas Bermeitinger, Robert Huber   +5 more
wiley   +1 more source

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

Genetically‐targeted control of the spinal cord and peripheral nervous system

Journal of Intelligent Medicine, EarlyView.
Application of genetically‐targeted stimulation for treating diseases or injuries outside the brain. Optogenetic and chemogenetic systems have been developed for dysfunction in the cochlea, spinal cord, dorsal root ganglia (DRGs), peripheral nerves, lower urinary tract, and enteric nervous system (ENS). Created with Biorender.com.
Anakaren Romero Lozano, Victoria Koptelova, Zoya Ahmad, Huiliang (Evan) Wang   +3 more
wiley   +1 more source

Capsid-dependent lentiviral restrictions

Journal of Virology
ABSTRACT Host antiviral proteins inhibit primate lentiviruses and other retroviruses by targeting many features of the viral life cycle. The lentiviral capsid protein and the assembled viral core are known to be inhibited through multiple, directly acting antiviral proteins.
Joy Twentyman, Michael Emerman, Molly Ohainle   +2 more
openaire   +4 more sources

The Function of TER94 of Spodoptera frugiperda 9 Cells When Infected With Invertebrate Iridescent Virus‐6

Journal of Basic Microbiology, EarlyView.
ABSTRACT The 118 L protein in the envelope of the Invertebrate iridescent virus 6 (IIV6) is responsible for binding to receptors on the surface of permissive cells. We previously elucidated its function by silencing its gene and neutralizing the protein with antibodies.
Kubra Zengin, Cihan Inan, Remziye Nalcacioglu, Zihni Demirbag   +3 more
wiley   +1 more source

Modeling Viral Capsid Assembly

Advances in chemical physics, 2013
I present a review of the theoretical and computational methodologies that have been used to model the assembly of viral capsids. I discuss the capabilities and limitations of approaches ranging from equilibrium continuum theories to molecular dynamics simulations, and I give an overview of some of the important conclusions about virus assembly that ...
openaire   +4 more sources

Annual dynamics and metagenomics of marine vesicles: One more layer of complexity in the dissolved organic fraction

Limnology and Oceanography, EarlyView.
Abstract Despite the role of extracellular vesicles (EVs) in genetic and biogeochemical exchange in the ocean, there is a notable dearth of studies delving into the seasonal annual dynamics of EVs in uncultured microbial communities. Here, quantitative data from the oligotrophic Mediterranean Sea revealed that EVs mL−1 ranged from 104 to 106 throughout
Nadefa Adda Nekrouf, Lucia Maestre‐Carballa, Monica Lluesma‐Gomez, Francisco Martinez‐Hernandez, Manuel Martinez‐Garcia   +4 more
wiley   +1 more source

Anti‐Pseudomonas Aeruginosa Bacteriophage Loaded Electrospun Fibers for Antibacterial Wound Dressings

Macromolecular Rapid Communications, EarlyView.
Multidrug resistant Pseudomonas (P.) aeruginosa is detected in many infected wounds and is challenging to treat with antibiotics. As an alternative, this work incorporates anti‐P. aeruginosa Neko phages into electrospun fibres to explore their potential for treating infected wounds.
Tian Ju, Jixuan Li, Andrew Weston, Giovanni Satta, Sara Bolognini, Mariagrazia Di Luca, Simon Gaisford, Gareth R. Williams   +7 more
wiley   +1 more source