Results 81 to 90 of about 177,162 (260)

Trypsin is associated with the rotavirus capsid and is activated by solubilization of outer capsid proteins

Journal of General Virology, 2005
The rotavirus capsid is made up of three concentric protein layers. The outer layer, consisting of VP7 and VP4, is lost during virus entry into the host cell. Rotavirus field isolates can be adapted to high-titre growth in tissue culture by treatment with trypsin and by supplementing the culture medium with trypsin, which cleaves VP4 into two fragments,
Benureau, Yann, Huet, Jean-Claude, Charpilienne, Annie, Poncet, Didier, Cohen, Jean   +4 more
openaire   +4 more sources

Computer Vision Pipeline for Image Analysis for Freeze‐Fracture Electron Microscopy: Rosette Cellulose Synthase Complexes Case

Advanced Intelligent Discovery, EarlyView.
This paper presents a computer vision (deep learning) pipeline integrating YOLOv8 and YOLOv9 for automated detection, segmentation, and analysis of rosette cellulose synthase complexes in freeze‐fracture electron microscopy images. The study explores curated dataset expansion for model improvement and highlights pipeline accuracy, speed ...
Siri Mudunuri, Leala Carbonneau, Eric M. Roberts, Alison W. Roberts, Candace H. Haigler, Alexey V. Gulyuk, Yaroslava G. Yingling   +6 more
wiley   +1 more source

Machine Learning‐Enhanced Random Matrix Theory Design for Human Immunodeficiency Virus Vaccine Development

Advanced Intelligent Discovery, EarlyView.
This study integrates random matrix theory (RMT) and principal component analysis (PCA) to improve the identification of correlated regions in HIV protein sequences for vaccine design. PCA validation enhances the reliability of RMT‐derived correlations, particularly in small‐sample, high‐dimensional datasets, enabling more accurate detection of ...
Mariyam Siddiqah, Muhammad Zulqarnain Zeb, Muhammad Husnain Zeb, Babar Shabbir   +3 more
wiley   +1 more source

Shotgun Metagenomic Profiling of the Gut Virome in Prodromal and Confirmed Parkinson's Disease

Annals of Neurology, EarlyView.
We conducted a nested case‐control study within the Nurses’ Health Study and the Health Professionals Follow‐up Study to examine the role of the gut virome (GV) in Parkinson's disease (PD). We applied a novel metagenomic virome profiling approach, Bioinformatic Application for Quantification and Labeling of Viral taxonomy (BAQLaVa), to prospectively ...
Deepika Dinesh, Xochitl C. Morgan, Jordan Jensen, Kjetil Bjornevik, Michael A. Schwarzschild, Alberto Ascherio, Curtis Huttenhower, Natalia Palacios   +7 more
wiley   +1 more source

Assembly of the Hv190S Totivirus Capsid Is Independent of Posttranslational Modification of the Capsid Protein

Virology, 1998
The genome of Helminthosporium victoriae 190S totivirus (Hv190SV) consists of two large overlapping open reading frames (ORFs), encoding a capsid protein (CP) and an RNA-dependent RNA polymerase. The capsid of Hv190SV, even though encoded by a single gene, contains three closely related capsid polypeptides: p88, p83, and p78.
Soldevila, Ana I., Huang, Shao, Ghabrial, Said A.   +2 more
openaire   +2 more sources

Antibody Competition Reveals Surface Location of HPV L2 Minor Capsid Protein Residues 17–36

Viruses, 2017
The currently available nonavalent human papillomavirus (HPV) vaccine exploits the highly antigenic L1 major capsid protein to promote high-titer neutralizing antibodies, but is limited to the HPV types included in the vaccine since the responses are ...
Stephanie M. Bywaters, Sarah A. Brendle, Kerstin P. Tossi, Jennifer Biryukov, Craig Meyers, Neil D. Christensen   +5 more
doaj   +1 more source

Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant

Biological Research, 2009
In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.
NELSON ACOSTA-RIVERO, JOANNA POUTOU, LIZ ÁLVAREZ-LAJONCHERE, IVIS GUERRA, YARAIMA AGUILERA, ALEXIS MUSACCHIO, ARMANDO RODRÍGUEZ, JULIO C AGUILAR, VIVIANA FALCON, JULIO C ÁLVAREZ-OBREGON, YORDANKA SORIA, DINORAH TORRES, MARBELIS LINARES, ÁNGEL PÉREZ, JUAN MORALES-GRILLO, SANTIAGO DUEÑAS -CARRERA   +15 more
doaj  

Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody

Animal Research and One Health, EarlyView.
This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng, Xuyan Xiang, Xinyu Qi, Hanrong Zhou, Peiyu Xiao, Fandan Meng, Tongqing An, Haiwei Wang   +7 more
wiley   +1 more source

Efficient Capsid Antigen Presentation From Adeno-Associated Virus Empty Virions In Vivo

Frontiers in Immunology, 2018
Adeno-associated virus (AAV) vectors have been successfully applied in clinical trials for hemophilic patients. Although promising, the clinical results suggest that the capsid-specific CD8+T cell response has a negative effect on therapeutic success. In
Xiaolei Pei, Xiaolei Pei, Lauriel Freya Earley, Yi He, Yi He, Xiaojing Chen, Nikita Elexa Hall, Richard Jude Samulski, Richard Jude Samulski, Chengwen Li, Chengwen Li   +10 more
doaj   +1 more source

Investigating the Heat Stability and Inactivation Conditions of Decapod Iridescent Virus 1 (DIV1)

Animal Research and One Health, EarlyView.
Heat treatment effectively inactivates decapod iridescent virus 1 (DIV1) under milder conditions than current WOAH recommendations. DIV1 infectivity was eliminated at 56°C for 30 min, 60°C for 15 min, or 70°C for 1 min, providing optimized heat treatment strategies for aquaculture biosecurity and seafood safety.
Yonghui Feng, Xiaomeng Guo, Jingyi Xing, Liang Qiu, Jie Huang   +4 more
wiley   +1 more source