Results 11 to 20 of about 216,910 (182)

Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features

Viruses, 2021
The capsid structures of most Adeno-associated virus (AAV) serotypes, already assigned to an antigenic clade, have been previously determined. This study reports the remaining capsid structures of AAV7, AAV11, AAV12, and AAV13 determined by cryo-electron
M. Mietzsch, Ariana Jose, P. Chipman, N. Bhattacharya, N. Daneshparvar, R. McKenna, M. Agbandje-McKenna   +6 more
semanticscholar   +1 more source

Structural and mechanistic bases for a potent HIV-1 capsid inhibitor

Science, 2020
Attacking HIV by stabilizing its capsid Current HIV treatments require drugs that must be taken daily, and care would be improved with an effective drug that is long-acting.
S. Bester, Guochao Wei, Haiyan Zhao, Daniel Adu-Ampratwum, N. Iqbal, Valentine V. Courouble, A. C. Francis, Arun S. Annamalai, P. Singh, Nikoloz Shkriabai, Peter van Blerkom, James H Morrison, E. Poeschla, A. Engelman, G. Melikyan, P. Griffin, J. Fuchs, F. Asturias, M. Kvaratskhelia   +18 more
semanticscholar   +1 more source

Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro

Science, 2020
HIV-1 replication and integration in vitro To infect a host cell, HIV-1 must reverse transcribe its single-stranded RNA genome into a double-stranded DNA copy and integrate that copy into a host chromosome. Reverse transcription and integration have been
Devin E. Christensen, B. K. Ganser-Pornillos, Jarrod S. Johnson, O. Pornillos, W. Sundquist   +4 more
semanticscholar   +1 more source

Adeno-associated virus capsid assembly is divergent and stochastic

Nature Communications, 2020
Adeno-associated viruses (AAVs) are increasingly used as gene therapy vectors. AAVs package their genome in a non-enveloped T = 1 icosahedral capsid of ~3.8 megaDalton, consisting of 60 subunits of 3 distinct viral proteins (VPs), which vary only in ...
Tobias P. Wörner, A. Bennett, Sana Habka, J. Snijder, O. Friese, Thomas W. Powers, M. Agbandje-McKenna, A. Heck   +7 more
semanticscholar   +1 more source

Identification of a myotropic AAV by massively parallel in vivo evaluation of barcoded capsid variants

Nature Communications, 2020
Adeno-associated virus (AAV) forms the basis for several commercial gene therapy products and for countless gene transfer vectors derived from natural or synthetic viral isolates that are under intense preclinical evaluation.
J. Weinmann, S. Weis, Josefine Sippel, W. Tulalamba, A. Remes, Jihad El Andari, A. Herrmann, Q. H. Pham, Christopher Borowski, S. Hille, T. Schönberger, N. Frey, M. Lenter, T. VandenDriessche, O. Müller, M. Chuah, T. Lamla, D. Grimm   +17 more
semanticscholar   +1 more source

Rhesus TRIM5α disrupts the HIV-1 capsid at the inter-hexamer interfaces [PDF]

, 2011
TRIM proteins play important roles in the innate immune defense against retroviral infection, including human immunodeficiency virus type-1 (HIV-1). Rhesus macaque TRIM5α (TRIM5αrh) targets the HIV-1 capsid and blocks infection at an early post-entry ...
A Desai, A Reymond, AK Kar, Angela M. Gronenborn, B Song, BK Ganser, BK Ganser-Pornillos, BK Ganser-Pornillos, BM Forshey, C Aiken, C Munk, CC Mische, Christopher Aiken, CJ Rold, CM Owens, CR Langelier, D Perez-Caballero, D Perez-Caballero, Danxia Ke, E Nogales, F Diaz-Griffero, F Diaz-Griffero, F Diaz-Griffero, F Diaz-Griffero, G Meroni, Gongpu Zhao, H Javanbakht, H Javanbakht, I Fujiwara, IJ Byeon, J Shi, JE Hinshaw, Jeremy Luban, Jinwoo Ahn, JM Phillips, K Ozato, K Wehrly, L Berthoux, LC James, Lisa M. Charlton, LR Black, M Stremlau, M Stremlau, M Stremlau, MJ Perron, MP Dodding, MW Yap, MW Yap, MW Yap, O Pornillos, P Gallay, Peijun Zhang, PV Maillard, Ruifeng Yang, S Nisole, S Ohkura, S Sebastian, SL Sawyer, Thomas Vu, Vaibhav B. Shah, X Li, X Li, X Wu, Y Durocher, Y Li   +64 more
core   +14 more sources

HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells

bioRxiv, 2020
HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization and mechanism of uncoating are under debate. We adapted a strategy to visualize individual reverse-transcribed HIV-1 cDNA molecules and their association with viral
Thorsten G. Müller, V. Zila, Kyra Peters, Sandra Schifferdecker, M. Stanić, Bojana Lucic, V. Laketa, M. Lusic, B. Müller, H. Kräusslich   +9 more
semanticscholar   +1 more source

Capsid protein structure in Zika virus reveals the flavivirus assembly process

Nature Communications, 2020
Structures of flavivirus (dengue virus and Zika virus) particles are known to near-atomic resolution and show detailed structure and arrangement of their surface proteins (E and prM in immature virus or M in mature virus). By contrast, the arrangement of
T. Y. Tan, G. Fibriansah, V. Kostyuchenko, T. Ng, X. Lim, Shuijun Zhang, Xin-Ni Lim, Jiaqi Wang, Jian Shi, M. Morais, D. Corti, S. Lok   +11 more
semanticscholar   +1 more source

Comprehensive AAV capsid fitness landscape reveals a viral gene and enables machine-guided design

Science, 2019
The fitness landscape of AAV Adeno-associated virus (AAV) is an important gene therapy vector. Using tools from synthetic biology, Ogden et al. provide a comprehensive view of how sequence changes in capsid proteins affect AAV properties.
Pierce J. Ogden, Eric D. Kelsic, S. Sinai, G. Church   +3 more
semanticscholar   +1 more source

Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR

Nature Structural & Molecular Biology, 2020
HIV-1 capsid plays multiple key roles in viral replication, and inhibition of capsid assembly is an attractive target for therapeutic intervention. Here, we report the atomic-resolution structure of capsid protein (CA) tubes, determined by magic-angle ...
Manman Lu, Ryan W. Russell, Alexander J. Bryer, Caitlin M. Quinn, Guangjin Hou, Huilan Zhang, C. Schwieters, J. Perilla, A. Gronenborn, T. Polenova   +9 more
semanticscholar   +1 more source