Results 21 to 30 of about 298,386 (364)

Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro

Science, 2020
HIV-1 replication and integration in vitro To infect a host cell, HIV-1 must reverse transcribe its single-stranded RNA genome into a double-stranded DNA copy and integrate that copy into a host chromosome. Reverse transcription and integration have been
Devin E. Christensen, B. K. Ganser-Pornillos, Jarrod S. Johnson, O. Pornillos, W. Sundquist   +4 more
semanticscholar   +1 more source

Electrostatic Screening, Acidic pH and Macromolecular Crowding Increase the Self-Assembly Efficiency of the Minute Virus of Mice Capsid In Vitro

Viruses, 2023
The hollow protein capsids from a number of different viruses are being considered for multiple biomedical or nanotechnological applications. In order to improve the applied potential of a given viral capsid as a nanocarrier or nanocontainer, specific ...
Miguel Angel Fuertes, Diego López Mateos, Luis Valiente, Alicia Rodríguez Huete, Alejandro Valbuena, Mauricio G. Mateu   +5 more
doaj   +1 more source

Identification of a myotropic AAV by massively parallel in vivo evaluation of barcoded capsid variants

Nature Communications, 2020
Adeno-associated virus (AAV) forms the basis for several commercial gene therapy products and for countless gene transfer vectors derived from natural or synthetic viral isolates that are under intense preclinical evaluation.
J. Weinmann, S. Weis, Josefine Sippel, W. Tulalamba, A. Remes, Jihad El Andari, A. Herrmann, Q. H. Pham, Christopher Borowski, S. Hille, T. Schönberger, N. Frey, M. Lenter, T. VandenDriessche, O. Müller, M. Chuah, T. Lamla, D. Grimm   +17 more
semanticscholar   +1 more source

A Narrative Review on Current Development and Future Prospects for the Treatment of Dengue Virus Infection [PDF]

Liaquat National Journal of Primary Care
Incidences of dengue-virus infection are increasing globally. Around 3.90 billion population is at high risk of infection. There is no cure/ prevention available.
Sheikh Abdul Khaliq, Muneer Khan, Saquib Azeem, Anab Fatima   +3 more
doaj   +1 more source

An extracellular vesicle targeting ligand that binds to Arc proteins and facilitates Arc transport in vivo

eLife, 2023
Communication between distant cells can be mediated by extracellular vesicles (EVs) that deliver proteins and RNAs to recipient cells. Little is known about how EVs are targeted to specific cell types.
Peter H Lee, Michael Anaya, Mark S Ladinsky, Justin M Reitsma, Kai Zinn   +4 more
doaj   +1 more source

HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells

bioRxiv, 2020
HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization and mechanism of uncoating are under debate. We adapted a strategy to visualize individual reverse-transcribed HIV-1 cDNA molecules and their association with viral
Thorsten G. Müller, V. Zila, Kyra Peters, Sandra Schifferdecker, M. Stanić, Bojana Lucic, V. Laketa, M. Lusic, B. Müller, H. Kräusslich   +9 more
semanticscholar   +1 more source

Capsid protein structure in Zika virus reveals the flavivirus assembly process

Nature Communications, 2020
Structures of flavivirus (dengue virus and Zika virus) particles are known to near-atomic resolution and show detailed structure and arrangement of their surface proteins (E and prM in immature virus or M in mature virus). By contrast, the arrangement of
T. Y. Tan, G. Fibriansah, V. Kostyuchenko, T. Ng, X. Lim, Shuijun Zhang, Xin-Ni Lim, Jiaqi Wang, Jian Shi, M. Morais, D. Corti, S. Lok   +11 more
semanticscholar   +1 more source

Nanomedicine and Phage Capsids [PDF]

Viruses, 2018
Studies of phage capsids have at least three potential interfaces with nanomedicine. First, investigation of phage capsid states potentially will provide therapies targeted to similar states of pathogenic viruses. Recently detected, altered radius-states of phage T3 capsids include those probably related to intermediate states of DNA injection and DNA ...
Philip Serwer, Elena T. Wright
openaire   +4 more sources

Genetic Code Expansion and Bio-Orthogonal Labeling Reveal Intact HIV-1 Capsids inside the Nucleus

mBio, 2022
Microscopy is one of the few techniques that can directly observe the HIV-1 capsid as it traverses the cell. However, an extrinsic or intrinsic label is needed to facilitate detection and this can perturb capsid behavior. Now, S. Schifferdecker, V. Zila,
Leo C. James
doaj   +1 more source

Comprehensive AAV capsid fitness landscape reveals a viral gene and enables machine-guided design

Science, 2019
The fitness landscape of AAV Adeno-associated virus (AAV) is an important gene therapy vector. Using tools from synthetic biology, Ogden et al. provide a comprehensive view of how sequence changes in capsid proteins affect AAV properties.
Pierce J. Ogden, Eric D. Kelsic, S. Sinai, G. Church   +3 more
semanticscholar   +1 more source