Results 21 to 30 of about 4,482,895 (292)

Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli

Scientific Reports, 2021
Signal peptides and secretory carrier proteins are commonly used to secrete heterologous recombinant protein in Gram-negative bacteria. The Escherichia coli osmotically-inducible protein Y (OsmY) is a carrier protein that secretes a target protein ...
David Gonzalez-Perez, James Ratcliffe, Shu Khan Tan, Mary Chen May Wong, Yi Pei Yee, Natsai Nyabadza, Jian-He Xu, Tuck Seng Wong, Kang Lan Tee   +8 more
doaj   +1 more source

A crucial role of the mitochondrial protein import receptor MOM19 for the biogenesis of mitochondria [PDF]

, 1994
The novel genetic method of "sheltered RIP" (repeat induced point mutation) was used to generate a Neurospora crassa mutant in which MOM19, a component of the protein import machinery of the mitochondrial outer membrane, can be depleted.
W Neupert, Lill, Roland, van der Klei, Ida, Neupert, Walter, Harkness, Troy A. A., Klei, Ida J. van der, TA Harkness, I van der Klei, van der Klei, Ida ; id_orcid, Nargang, Frank E., FE Nargang, R Lill   +11 more
core   +2 more sources

Biological Activity of the Carrier as a Factor in Immunogen Design for Haptens

Molecules, 2018
Immunoanalytical methods are frequently employed in the detection of hazardous small molecular weight compounds. However, antibody development for these molecules is a challenge, because they are haptens and cannot induce a humoral immune response in ...
Özlem Ertekin, Esin Akçael, Harun Kocaağa, Selma Öztürk   +3 more
doaj   +1 more source

Conjugation Mechanism for Pneumococcal Glycoconjugate Vaccines: Classic and Emerging Methods

Bioengineering, 2022
Licensed glycoconjugate vaccines are generally prepared using native or sized polysaccharides coupled to a carrier protein through random linkages along the polysaccharide chain.
Victor Morais, Norma Suarez
doaj   +1 more source

Lipid carrier proteins and ethanol

Journal of Biomedical Science, 2001
Ethanol has a pronounced effect on lipid homeostasis. It is our overall hypothesis that certain lipid carrier proteins are targets of acute and chronic ethanol exposure and that perturbation of these proteins induces lipid dysfunction leading to cellular pathophysiology. These proteins include both intracellular proteins and lipoproteins.
W G, Wood, N A, Avdulov, S V, Chochina, U, Igbavboa   +3 more
openaire   +2 more sources

MOM19, an import receptor for mitochondrial precursor proteins [PDF]

, 1989
We have identified a 19 kd protein of the mitochondrial outer membrane (MOM19). Monospecific IgG and Fab fragments directed against MOM19 inhibit import of precursor proteins destined for the various mitochondrial subcompartments, including porin ...
Söllner, Thomas, Pfaller, Rupert, Pfanner, Nikolaus, Neupert, Walter, Griffiths, Gareth   +4 more
core   +1 more source

Study of interaction between nicotinamide and human serum albumin using spectroscopic techniques and molecular docking simulation simulation

یافته‌های نوین در علوم زیستی, 2022
Human serum albumin is one of the most important blood proteins that has the ability to bind a wide range of compounds and different drugs. Hence, knowing how drugs bind to albumin is crucial to understand their pharmacokinetics and pharmacodynamic ...
Golnaz Parvizi Fard, Lale Solouki, Mostafa Zakariazadeh, Hossein Haghaei, Somaieh Soltani   +4 more
doaj  

Characterization and Comparison of Ochratoxin A-Ovalbumin (OTA-OVA) Conjugation by Three Methods

Journal of Integrative Agriculture, 2014
In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (ochratoxin A, OTA) was coupled to ovalbumin (OVA) by an active ester reaction. To develop
Xi-chun WANG, Ming BAO, Jin-jie WU, Ying LUO, Liang-you MA, Ying WANG, Ai-hua ZHANG, Cheng-hua HE, Hai-bin ZHANG   +8 more
doaj   +1 more source

Moco Carrier and Binding Proteins

Molecules, 2022
The molybdenum cofactor (Moco) is the active site prosthetic group found in numerous vitally important enzymes (Mo-enzymes), which predominantly catalyze 2 electron transfer reactions. Moco is synthesized by an evolutionary old and highly conserved multi-step pathway, whereby the metal insertion reaction is the ultimate reaction step here. Moco and its
openaire   +3 more sources

The Suppressor of AAC2 Lethality SAL1 Modulates Sensitivity of Heterologously Expressed Artemia ADP/ATP Carrier to Bongkrekate in Yeast [PDF]

, 2013
The ADP/ATP carrier protein (AAC) expressed in Artemia franciscana is refractory to bongkrekate. We generated two strains of Saccharomyces cerevisiae where AAC1 and AAC3 were inactivated and the AAC2 isoform was replaced with Artemia AAC containing a ...
Adam-Vizi, Vera, Monika Wysocka-Kapcinska (368157), Christos Chinopoulos (317665), Roza Kucharczyk (141980), Christos Chinopoulos, Cynthia L David, Chinopoulos Christos, Hunt AM, Vekey, Karoly, Beata Torocsik (317661), Hunt Andrea M., Roza Kucharczyk, Karoly Vekey (5656285), Kucharczyk Roza, Wysocka-Kapcinska M, Cynthia L. David, Vera Adam-Vizi (317663), Vékey Károly, Cynthia L. David (461150), Törőcsik Beáta, Ádám Veronika, Monika Wysocka-Kapcinska, Andrea M. Hunt, Wysocka-Kapcinska, Monika, Lilla Turiak, Tsaprailis, George, David Cynthia L., Törőcsik, Beáta, Turiák, Lilla, Tsaprailis George, Andrea M. Hunt (461151), Wysocka-Kapcinska Monika, Beata Torocsik, Vera Adam-Vizi, George Tsaprailis (268492), Ádám, Veronika, Kucharczyk R, Andrea M Hunt, David CL, George Tsaprailis, Tsaprailis G, Chinopoulos, Christos, Turiák Lilla, Hunt, Andrea M., Lilla Turiak (5656282), Kucharczyk, Roza, David, Cynthia L., Karoly Vekey, Adam-Vizi Vera   +48 more
core   +2 more sources