Results 171 to 180 of about 251,083 (336)

Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

Molecular Therapy: Nucleic Acids, 2018
The Cas9/guide RNA (Cas9/gRNA) system is commonly used for genome editing. mRNA expressing Cas9 can induce innate immune responses, reducing Cas9 expression.
Sriram Vaidyanathan, Krist T. Azizian, A.K.M. Ashiqul Haque, Jordana M. Henderson, Ayal Hendel, Sabrina Shore, Justin S. Antony, Richard I. Hogrefe, Michael S.D. Kormann, Matthew H. Porteus, Anton P. McCaffrey   +10 more
doaj  

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA [PDF]

, 2014
Suresh Ramakrishna, Abu-Bonsrah Kwaku Dad, Jagadish Beloor, Ramu Gopalappa, Sang‐Kyung Lee, Seokjoong Kim   +5 more
openalex   +1 more source

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations

Advanced Science, EarlyView.
CRISPR/Cas9, while transformative, faces challenges in specificity, precision, delivery, accessibility, flexibility, and safety. This review addresses these limitations by highlighting strategies to reduce off‐target effects, exploring HDR‐based and alternative editing approaches, and evaluating advanced delivery mechanisms.
Muna Alariqi, Mohamed Ramadan, Lu Yu, Fengjiao Hui, Amjad Hussain, Xiaofeng Zhou, Yu Yu, Xianlong Zhang, Shuangxia Jin   +8 more
wiley   +1 more source

JA‐Mediated Regulation of Amino Acid Homeostasis Adjusts Metabolic Flux and Enhances Spider Mite Tolerance via the SlJAZ8‐SlWRKY57‐SlAVT6s Module in Tomato

Advanced Science, EarlyView.
This study elucidates a novel SlJAZ8‐SlWRKY57‐SlAVT6A/SlAVT6B module that effectively channels metabolic flux from primary to secondary metabolism, impacting plant growth, development, and resistance. Two novel vacuolar amino acid exporters, SlAVT6A and SlAVT6B, fine‐tunes trichomes, terpenes, and gibberellins, bolstering spider mite resistance.
Yingchen Hao, Xiaolong Wang, Langchen Guo, Lijun Xiang, Enxi Luo, Peng Cao, Penghui Liu, Yue Zhong, Chun Li, Jun Lai, Jun Yang, Shouchuang Wang   +11 more
wiley   +1 more source

Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Nature Communications
The CRISPR-Cas9 system has frequently been used for genome editing in Streptomyces; however, cytotoxicity, caused by off-target cleavage, limits its application.
Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh   +7 more
doaj   +1 more source

CRISPRs extending their reach: prokaryotic RNAi protein Cas9 recruited for gene regulation

, 2013
Nadja Heidrich, Jörg Vogel
openalex   +2 more sources

Human immunity to Cas9 [PDF]

Nature Methods, 2019
openaire   +2 more sources

LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma

Advanced Science, EarlyView.
In SETD2‐mutant ccRCC, the original LEDGF recognition of the H3K36me3 regulatory axis no longer exists. LEDGF interacts with CARM1‐dependent H3R17me2a regulating the transcription of key enzymes in the de novo synthesis pathway. The abnormally elevated LEDGF leads to the accumulation of purine nucleotides in SETD2‐mutant ccRCC, thereby satisfying the ...
Yuwei Zhang, Yuhua Zhou, Yuezhou Zhang, Jing Lv, Yang Shen, Dong Zhang, Bo Liu, Wei Zhao, Junyi Ju, Qingyi Zhu, Ke Wang, Ninghan Feng   +11 more
wiley   +1 more source

crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus

, 2013
Tautvydas Karvelis, Giedrius Gasiūnas, Algirdas Miksys, Rodolphe Barrangou, Philippe Horvath, Virginijus Šikšnys   +5 more
openalex   +1 more source

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

, 2014
Samuel H. Sternberg, Sy Redding, Martin Jínek, Eric C. Greene, Jennifer A. Doudna   +4 more
openalex   +3 more sources