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Nature Reviews Molecular Cell Biology, 2020
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
exaly +3 more sources
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
exaly +3 more sources
2023
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
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CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +3 more sources
2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
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CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +3 more sources
Nature Biotechnology, 2015
Cpf1 is one of a growing number of class II CRISPR-Cas effectors that expand both our understanding of bacterial immunity and our genome-editing toolset.
Erik J, Sontheimer, Scot A, Wolfe
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Cpf1 is one of a growing number of class II CRISPR-Cas effectors that expand both our understanding of bacterial immunity and our genome-editing toolset.
Erik J, Sontheimer, Scot A, Wolfe
openaire +2 more sources
2020
?? ???????????? ?????????????? ?????? ?????????????? ??????????????????, ?????????????????? ???????????????? ?? ???????????????? ?????????????????????? ???????????????????????? ???????????? ?????????????????? ?????????????????????? ?????? ?????????????????????? ???????????? ??? CRISPR/Cas9. ???????????? ???? ???????????? ???????? ?? ?????????????????? ?
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?? ???????????? ?????????????? ?????? ?????????????? ??????????????????, ?????????????????? ???????????????? ?? ???????????????? ?????????????????????? ???????????????????????? ???????????? ?????????????????? ?????????????????????? ?????? ?????????????????????? ???????????? ??? CRISPR/Cas9. ???????????? ???? ???????????? ???????? ?? ?????????????????? ?
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Nature Cell Biology, 2019
MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
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MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
openaire +2 more sources
2018
In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation.
Jakob B, Hoof +2 more
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In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation.
Jakob B, Hoof +2 more
openaire +2 more sources