Cas9 - Open Access .click

Results 281 to 290 of about 251,083 (336)

A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9

, 2015
Kelli J. Carroll +8 more
openalex +1 more source

Akkermansia Muciniphila Alleviates Severe Acute Pancreatitis via Amuc1409‐Ube2k‐Foxp3 Axis in Regulatory T Cells

Advanced Science, EarlyView.
Akkermansia depletion correlates with acute pancreatitis (AP) severity. A. muciniphila and its protein Amuc_1409 mitigate pancreatic/systemic inflammation by expanding Tregs and IL‐10. Mechanistically, Amuc_1409 binds Ube2k, inhibiting Foxp3 ubiquitination to stabilize Treg differentiation and IL‐10 production, countering SAP‐induced SIRS/CARS ...
Jinyan Xie +9 more
wiley +1 more source

Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation

Advanced Science, EarlyView.
The engineered calprotectin biosensor detects gut inflammation, processes the signal through genetic regulation, and responds by expressing the reporter sfGFP and the therapeutic protein secIL10. This dual functionality enables the engineered biosensor not only to sense but also to mitigate gut inflammation, highlighting its potential for disease ...
Duolong Zhu +8 more
wiley +1 more source

Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9. [PDF]

Elife
Belato HB +8 more
europepmc +1 more source

The E3 Ligase NEDD4L Prevents Colorectal Cancer Liver Metastasis via Degradation of PRMT5 to Inhibit the AKT/mTOR Signaling Pathway

Advanced Science, EarlyView.
Neural precursor cell expressed developmentally down‐regulated gene 4‐like (NEDD4L) prevents colorectal cancer liver metastasis through ubiquitination and degradation of protein arginine methyltransferase 5 (PRMT5). PRMT5 degradation attenuates the methylarginine of AKT1.
Zhewen Dong +8 more
wiley +1 more source

Advances in Genome Editing Through Haploid Induction Systems. [PDF]

Int J Mol Sci
Sheng H +6 more
europepmc +1 more source

High-Throughput Silencing Using the CRISPR-Cas9 System: A Review of the Benefits and Challenges

, 2015
Mark Wade
openalex +1 more source

An Amygdala‐hippocampus Circuit for Endocannabinoid Modulation of Anxiety Avoidance

Advanced Science, EarlyView.
This study employs three synapse‐specific endocannabinoids (eCB) probes to monitor dynamic eCB release, activate CB1Rs and knockdown 2‐AG biosynthesis enzymes in glutamatergic anterior basolateral amygdala ‐ ventral hippocampus (aBLA–vHPC) circuit. At aBLA–vHPC circuits, activation of CB1Rs reduce anxiety avoidance, and knockdown of eCB biosynthesis ...
Bao Xue +9 more
wiley +1 more source

Monitoring the Dynamics of Alzheimer's Disease Biomarkers and the APOE–Tau Axis via Human Cerebral Organoids with Immuno‐SERS

Advanced Science, EarlyView.
Noninvasive and sensitive detection of tau protein dynamics across developmental stages and APOE genotypes remain a challenge in Alzheimer's disease (AD) research. This study reveals the dynamics of tau secretion in label‐free manner using surface‐enhanced Raman spectroscopy (SERS) and human cerebral organoids (hCOs). This study provides novel insights
Yongjae Jo +11 more
wiley +1 more source

Advanced viral genome in vitro Cas9 editing (AdVICE): an overnight method for traceless and limitless manipulation of adenoviral and vector genomes with large transgenes. [PDF]

J Virol
Vergnes J-B, Roger B, Iggo R, Wodrich H.
europepmc +1 more source

crispr
genetics
biology

gene
genome editing
computational biology

crispr-cas systems
gene editing
rna