Results 291 to 300 of about 251,083 (336)

Engineering Adenine Deaminase TadA for Precise and PAM‐Flexible Point Mutagenesis and Gradient‐Tuning Endogenous Protein Design

Advanced Science, EarlyView.
Base editing enables precise nucleotide substitutions but limited by bystander editing. This study engineers plant base editors by fusing optimized TadA variants with PAM‐flexible SpRY nickase, enabling A‐to‐G, C‐to‐T, and dual‐base conversions in a highly condensed window (≤3 nucleotides). Additionally, TadDBE (TadA Dual‐Base Editor)‐mediated directed
Kangli Sun, Si Cheng, Nan Chai, Jianing Mi, Ruixiang Zhang, Qian Qian, Zhiye Zheng, Ke Chen, Dongchang Zeng, Xin Peng, Mengyuan Shen, Degui Zhou, Qinlong Zhu, Qi Liu, Jiantao Tan   +14 more
wiley   +1 more source

Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement. [PDF]

Stress Biol
Mukhtiar A, Ullah S, Yang B, Jiang YQ.
europepmc   +1 more source

ASO Therapy Targeting STAU2 to Inhibit Pancreatic Ductal Adenocarcinoma Progression and Metastasis by Regulating the PALLD‐Mediated EMT Signaling Pathway

Advanced Science, EarlyView.
This study identifies STAU2 as an oncogenic RNA‐binding protein (RBP) with elevated expression in pancreatic ductal adenocarcinoma (PDAC), where its high levels are significantly correlated with metastatic progression. It elucidates that STAU2 directly binds and regulates PALLD, while mediating the functional role of IQGAP1, thereby driving metastasis ...
Jiayu Ding, Hao Shen, Jiaying Ji, Jiaxing Li, Zhongrui Shi, Xuejiao Wang, Bangbang Li, Yi Hou, Wenjian Min, Chengliang Sun, Kai Yuan, Yasheng Zhu, Liping Wang, Shun‐Qing Liang, Wenbin Kuang, Xiao Wang, Peng Yang   +16 more
wiley   +1 more source

Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines. [PDF]

PLoS One
Wei SC, Cantor AJ, Walleshauser J, Mepani R, Melton K, Bans A, Khekare P, Gupta S, Wang J, Soares C, Kiwan R, Lee J, McCawley S, Jani V, Leong WI, Shahi PK, Chan J, Boivin P, Otoupal P, Pattnaik BR, Gamm DM, Saha K, Gowen BG, Haak-Frendscho M, Janatpour MJ, Silverman AP.   +25 more
europepmc   +1 more source

Cell Membrane‐Coated Lipid Nanoparticles for Drug Delivery

Aggregate, EarlyView.
This review highlights recent progress in cell membrane‐coated lipid nanoparticles (CMC‐LNPs), focusing on their design, preparation methods, functional integration, and biomedical applications. It discusses various types of LNPs and coating strategies, characterization techniques, therapeutic functions, and applications.
Moataz B. Zewail, Guangze Yang, Yilong Fan, Yue Hui, Chun‐Xia Zhao, Yun Liu   +5 more
wiley   +1 more source

Generation of transgene-free genome-edited carrot plants using CRISPR/Cas9-RNP complexes. [PDF]

Plant Cell Rep
Yarra R, Krysan PJ.
europepmc   +1 more source

Harnessing the Biological Responses Induced by Nanomaterials for Enhanced Cancer Therapy

Aggregate, EarlyView.
Nanomaterial (NM)‐induced toxicity can be strategically repurposed for cancer therapy. This review summarizes the mechanism by which NMs selectively activate specific cellular processes to regulate cell fate independently. We also discussed how NMs‐induced biological responses can be leveraged as therapeutic strategies for cancer treatment.
Liting Wang, Hao Fu, Jiangtao Lin, Meng Zhao, Chuanrong Chen, Hongze Liao, Yourong Duan   +6 more
wiley   +1 more source

Weaponizing CRISPR/Cas9 for selective elimination of cells with an aberrant genome.

DNA Repair (Amst)
Tavella S, di Lillo A, Conti A, Iannelli F, Mancheno-Ferris A, Matti V, Di Micco R, Fagagna FDD.   +7 more
europepmc   +1 more source

Utilization of Native CRISPR-Cas9 System for Expression of Glucagon-like Peptide-1 in <i>Lacticaseibacillus paracasei</i>. [PDF]

Foods
Zheng M, Zhang S, Wang Y, Xie N, Wang X, Lv J, Pang X, Li X.   +7 more
europepmc   +1 more source

Compound Heterozygous Variants in ZSWIM7 Gene Linked to Infertility and Its Role in Gonadal Development

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT This study aims to elucidate the role of the ZSWIM7 gene in the etiology of infertility, focusing on its potential association with premature ovarian insufficiency and azoospermia. We investigated an 18‐year‐old female patient who presented with primary amenorrhea, hypoplasia of the uterus and ovaries, Tanner stage II breast and pubic hair ...
Denise M. Christofolini, Guilherme Pinn, Thainá Vilella, Maria I. Melaragno, Mariana Moysés‐Oliveira, Luciano Pompei, Rare Genomes Project Consortium, Bianca Bianco, Caio P. Barbosa   +8 more
wiley   +1 more source