Results 81 to 90 of about 194,133 (208)

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

Nature Network Boston, 2018
Here, we report that genome editing by CRISPR–Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway.
E. Haapaniemi, Sandeep K Botla, Jenna Persson, B. Schmierer, J. Taipale   +4 more
semanticscholar   +1 more source

Fast Antibiotic resistance-Based gene editing of mammalian cells with CRISPR-Cas9 (FAB-CRISPR) [PDF]

arXiv
Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing low knock-in rates. We present a detailed pipeline using HDR donor plasmids containing antibiotic resistance cassettes for rapid selection of gene-edited cells.
arxiv  

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

Bioinform., 2014
Summary: The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system is an adaptive immune response in prokaryotes, protecting host cells against invading phages or plasmids by cleaving these foreign DNA species in a ...
Sangsu Bae, Jeongbin Park, Jin-Soo Kim
semanticscholar   +1 more source

Single step production of Cas9 mRNA for zygote injection

BioTechniques, 2018
Production of Cas9 mRNA in vitro typically requires the addition of a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region.
Bethany K Redel, Benjamin P Beaton, Lee D Spate, Joshua A Benne, Stephanie L Murphy, Chad W O’Gorman, Anna M Spate, Randall S Prather, Kevin D Wells   +8 more
doaj   +1 more source

Genome Engineering ofDrosophilawith the CRISPR RNA-Guided Cas9 Nuclease [PDF]

, 2013
Scott J. Gratz, Alexander M Cummings, Jennifer N. Nguyen, Danielle C. Hamm, Laura K. Donohue, Melissa M. Harrison, Jill Wildonger, Kate M. O’Connor-Giles   +7 more
openalex   +1 more source

Development and testing of a versatile genome editing application reporter (V-GEAR) system

Molecular Therapy: Methods & Clinical Development
CRISPR-Cas9 and novel cas fusion proteins leveraging specific DNA targeting ability combined with deaminases or reverse transcriptases have revolutionized genome editing.
Evan W. Kleinboehl, Kanut Laoharawee, Walker S. Lahr, Jacob D. Jensen, Joseph J. Peterson, Jason B. Bell, Beau R. Webber, Branden S. Moriarity   +7 more
doaj  

Target search in the CRISPR/Cas9 system: Facilitated diffusion with target cues [PDF]

arXiv
We study how Cas9, a central component of the CRISPR/Cas9 system, searches for a target sequence on the DNA. We propose a model that includes as key ingredients 3D diffusion, 1D sliding along the DNA, and the effect of short binding sequences preceding the target (protospacer adjacent sequences -- PAMs).
arxiv  

Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9 [PDF]

, 2013
Yuxuan Wu, Dan Liang, Yinghua Wang, Meizhu Bai, Wei Tang, Shiming Bao, Zhiqiang Yan, Dangsheng Li, Jinsong Li   +8 more
openalex   +1 more source

Recent Advances in Genome Editing Using CRISPR/Cas9

Frontiers in Plant Science, 2016
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence.
Yuduan eDing, Yuduan eDing, Hong eLi, Chen eLing-Ling, Chen eLing-Ling, Kabin eXie, Kabin eXie   +6 more
doaj   +1 more source

DeepFM-Crispr: Prediction of CRISPR On-Target Effects via Deep Learning [PDF]

arXiv
Since the advent of CRISPR-Cas9, a groundbreaking gene-editing technology that enables precise genomic modifications via a short RNA guide sequence, there has been a marked increase in the accessibility and application of this technology across various fields.
arxiv