Results 61 to 70 of about 228,880 (307)
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Concerning RNA-guided gene drives for the alteration of wild populations
eLife, 2014 Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here we consider the potential for RNA-guided gene drives based on
Kevin M Esvelt +3 more
doaj +1 more source
Cas9-mediated switching in human B cells.
, 2019 (A) Schematic depicting Cas9 variants and orientation of sgRNAs required to generate blunt, 5’, and 3’DSBs. One sgRNA and wild-type Cas9 produces a blunt DSB, while paired sgRNAs and Cas9 D10A or Cas9 N863A give rise to 5’ or 3’DSBs, respectively.
Alberto Martin (12795) +1 more
core +1 more source
Improved Cas9 activity by specific modifications of the tracrRNA [PDF]
, 2019 CRISPR/Cas is a transformative gene editing tool, that offers a simple and effective way to target a catalytic Cas9, the most widely used is derived from Streptococcus pyogenes (SpCas9), with a complementary small guide RNA (sgRNA) to inactivate ...
Ryan Urak +7 more
core +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Secondary Conformational Checkpoint in CRISPR-Cas9
A specific checkpoint between target DNA binding and cleavage primarily governs the precision of Cas9 gene editing. Although various CRISPR-Cas9 variants have been developed to improve DNA cleavage accuracy, we still lack a comprehensive understanding of
Shuxin Zhao (18387413) +2 more
core +2 more sources
Molecular Therapy: Nucleic Acids, 2022 The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park +8 more
doaj +1 more source
Cas9-mediated IGH-BCL6 translocations (der3) in human B cells.
, 2019 (A) Schematic depicting sgRNA target regions in IGH on human chromosome 14 and BCL6 on human chromosome 3. Primer binding sites are denoted by arrows and their sequences are presented in S1 Table.
Alberto Martin (12795) +1 more
core +1 more source
FEBS Letters, EarlyView.This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee +2 more
wiley +1 more source
Gene disruption rate mediated by using eleven different tRNA promoters in ANEp8-Cas9-gRNA plasmid.
, 2018 Gene disruption rate mediated by using eleven different tRNA promoters in ANEp8-Cas9-gRNA plasmid.
Jean-Paul Ouedraogo (3344108) +4 more
core +1 more source