Results 71 to 80 of about 228,880 (307)

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Current Applications and Future Perspectives of CRISPR-Cas9 for the Treatment of Lung Cancer

Biologics: Targets & Therapy, 2021
Markeshaw Tiruneh G/Medhin,1 Endeshaw Chekol Abebe,2 Tekeba Sisay,3 Nega Berhane,3 Tesfahun Bekele Snr,1 Tadesse Asmamaw Dejenie1 1Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar ...
Tiruneh G/Medhin M, Chekol Abebe E, Sisay T, Berhane N, Bekele Snr T, Asmamaw Dejenie T   +5 more
doaj  

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

CRISPR–Cas9 Based Bacteriophage Genome Editing

Microbiology Spectrum, 2022
Bacteriophages are the most abundant entities in the biosphere, and many genomes of rare and novel bacteriophages have been sequenced to date. However, bacteriophage functional genomics has been limited by a lack of effective research methods.
Xueli Zhang, Chaohui Zhang, Caijiao Liang, Bizhou Li, Fanmei Meng, Yuncan Ai   +5 more
doaj   +1 more source

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Development and testing of a versatile genome editing application reporter (V-GEAR) system

Molecular Therapy: Methods & Clinical Development
CRISPR-Cas9 and novel cas fusion proteins leveraging specific DNA targeting ability combined with deaminases or reverse transcriptases have revolutionized genome editing.
Evan W. Kleinboehl, Kanut Laoharawee, Walker S. Lahr, Jacob D. Jensen, Joseph J. Peterson, Jason B. Bell, Beau R. Webber, Branden S. Moriarity   +7 more
doaj   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

Molecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi, Tuan Khang Huynh, Adway Kadam, Zahra Kardan, Yue Yan, Amina Jamal Laham, Sampath Kumar Loganathan   +6 more
wiley   +1 more source

Generation of ADCK1 knock-out cells by CRISPR/Cas9 [PDF]

, 2022
openIntroduzione Il sistema CRISPR-Cas9 è una tecnica di editing genetico che si basa sull’utilizzo di una guida a singolo filamento di RNA (single-guide RNA, sgRNA) specifica, che guida l’endonucleasi Cas9 verso una regione di DNA target affinche ...
ZAMPIERI, SARA
core  

Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9

, 2023
International audienceThe CRISPR-Cas9 system has revolutionized our ability to precisely modify the genome and has led to gene editing in clinical applications.
G. Cullot, J. M. Blouin, S. Amintas, A. Bedel, Bedel, A., Rosier, J., Laharanne, E., S. Fayet, Cappellen, D., E. Richard, Prat, F., F. Prat, D. Cappellen, Moreau-Gaudry, F., Blouin, J, M, Guyonnet-Duperat, V., J. Boutin, Bouron, J., J. Rosier, C. Thibault, Dabernat, S., J. Bouron, S. Dabernat, Boutin, J., V. Guyonnet-Duperat, Amintas, S., I. Lamrissi, P. Pennamen, Cullot, G., F. Moreau-Gaudry, J. P. Merlio, Moranvillier, I., Pennamen, P., Fayet, S., Lamrissi, I., I. Moranvillier, Merlio, J, P, Richard, E., Thibault, C., E. Laharanne   +39 more
core   +1 more source

A role for eIF5A in translational control of stress‐inducible proteins in multiple myeloma cell lines

Molecular Oncology, EarlyView.
Loss of eIF5A rewires thapsigargin‐mediated endoplasmic reticulum stress responses in multiple myeloma cells by selective translation of mRNA transcripts, consequently dampening thapsigargin‐induced unfolded protein response (UPR) activation and shifting the balance from induction of apoptosis toward cell survival and proliferation.
Demi Both, Ingrid A. M. Derks, Raphaël Vanleyssem, Marine Leclercq, Najla El Hachem, Pierre Close, Eric Eldering, Marco Vincent Haselager   +7 more
wiley   +1 more source