Results 51 to 60 of about 228,880 (307)

Off- and on-target effects of genome editing in mouse embryos

The Journal of Reproduction and Development, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE, Kenichi NAKASHIMA, Atsushi YOSHIKI   +2 more
doaj   +1 more source

Advances in CRISPR/Cas9

BioMed Research International, 2022
CRISPR/Cas9 technology has become the most examined gene editing technology in recent years due to its simple design, yet low cost, high efficiency, and simple operation, which can also achieve simultaneous editing of multiple loci. It can also be carried out without using plasmids, saving lots of troubles caused by plasmids.
openaire   +2 more sources

Table3_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells

Genes and Diseases, 2022
The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to ...
Renke Tan, Wenhao Du, Yiyang Liu, Xiaoji Cong, Meirong Bai, Chenxiao Jiang, Zengxia Li, Minjia Tan, Dengke K. Ma, Qiang Huang, Wei Jiang, Yongjun Dang   +11 more
doaj   +1 more source

DataSheet1_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

Additional file 1 of CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition

, 2020
Additional file 1. gRNA sequences and potential off-target loci: Off-target sites as identified by Integrated DNA Technologies’ CRISPR-Cas9 gRNA Design Checker with mismatches (#MM) threshold set to 3.
L. Shaw (6427883), R. Williams (3511298), K. Hamill (8480826)   +2 more
core   +1 more source

Table2_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

The Versatile Tool: CRISPR/Cas12a System for Nucleic Acid Detection

Shengwu huaxue yu shengwu wuli jinzhan
The CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas). The system forms an adaptive immune system in archaea and bacteria.
DANG Sheng, ZHANG Shuai, ZHAI Jing-Bo
doaj   +1 more source

Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination

, 2021
Genetic engineering of industrial cell lines often requires knockout of multiple endogenous genes. Tools like CRISPR-Cas9 have enabled serial or parallelized gene disruption in a wide range of industrial organisms, but common practices for the screening ...
Neil C. Dalvie (3287133), Timothy Lorgeree, Timothy Lorgeree (11820308), Andrew M. Biedermann, Kerry R. Love (4490911), Neil C. Dalvie, Andrew M. Biedermann (10735208), Kerry R. Love, J. Christopher Love, J. Christopher Love (159667)   +9 more
core   +1 more source