Results 41 to 50 of about 228,880 (307)

Table4_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA binding

Life Science Alliance, 2022
Cas9 is sumoylated and ubiquitylated in human cells. K848 is the major SUMO2/3 modification site, but multiple lysines are ubiquitylated, precipitating proteasomal degradation.
Tunahan Ergünay, Özgecan Ayhan, Arda B Celen, Panagiota Georgiadou, Emre Pekbilir, Yusuf T Abaci, Duygu Yesildag, Mandy Rettel, Ulduz Sobhiafshar, Anna Ogmen, NC Tolga Emre, Umut Sahin   +11 more
doaj   +1 more source

Table1_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology

Zhongliu Fangzhi Yanjiu, 2020
Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells).
XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng   +3 more
doaj   +1 more source

Computationally designed hyperactive Cas9 enzymes

Nature Communications, 2022
The ability to alter the genomes of living cells is key to understanding how genes influence the functions of organisms and will be critical to modify living systems for useful purposes. Here, the authors use computational design to discover Cas9 enzymes
Pascal D. Vos, Giulia Rossetti, Jessica L. Mantegna, Stefan J. Siira, Andrianto P. Gandadireja, Mitchell Bruce, Samuel A. Raven, Olga Khersonsky, Sarel J. Fleishman, Aleksandra Filipovska, Oliver Rackham   +10 more
doaj   +1 more source

Cas9-triggered chain ablation of cas9 as a gene drive brake [PDF]

Nature Biotechnology, 2016
With the advent of clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) technology, researchers can construct gene drives that can bias the inheritance of edited alleles to alter entire populations. As demonstrated with the mutagenic chain reaction in Drosophila4, the CRISPR-Cas9 system can propagate ...
Wu, Bing, Luo, Liqun, Gao, Xiaojing J.
openaire   +3 more sources

CRISPR/Cas9 i rak

Veterinarska stanica, 2021
CRISPR/Cas9 postala je snažna metoda za unošenje promjena u genom brojnih organizama. Prvi put otkriven u bakterijama kao dio prilagodljivog imunološkog sustava, CRISPR/Cas9 i njegove modificirane verzije u širokoj su uporabi u inženjeringu genoma i aktivaciji ili suzbijanju ekspresije gena.
Mimoune, Nora, Bahouh, Mohamed Wail, Boukhechem, Said, Khelef, Djamel, Kaidi, Rachid, Baazizi, Ratiba   +5 more
openaire   +2 more sources

Table5_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

DataSheet2_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.xlsx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

Delivering CRISPR: a review of the challenges and approaches

Drug Delivery, 2018
Gene therapy has long held promise to correct a variety of human diseases and defects. Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the mechanism of the CRISPR-based prokaryotic adaptive immune system (CRISPR ...
Christopher A. Lino, Jason C. Harper, James P. Carney, Jerilyn A. Timlin   +3 more
doaj   +1 more source