Results 21 to 30 of about 228,880 (307)
Journal of Oral Microbiology, 2022 Introduction Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems are RNA-mediated adaptive immune systems that actagainst invading genetic elements such as phages or plasmids.
Harumi Mizuki +3 more
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Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]
, 2015 CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro +5 more
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Smash and DASH with Cas9 [PDF]
Genome Biology, 2016 DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
Ramani, Vijay, Shendure, Jay
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Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9 [PDF]
, 2014 This work was supported in part by NIH grant 1DP5OD017892 to S.LToxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the
Saima M Sidik +20 more
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G3: Genes, Genomes, Genetics, 2020 Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis. Transcriptional interference occurs at a
Victoria Jorgensen +12 more
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In Vitro Enzymology of Cas9 [PDF]
, 2014 Cas9 is a bacterial RNA-guided endonuclease that uses base pairing to recognize and cleave target DNAs with complementarity to the guide RNA. The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms.
Carolin Anders, Martin Jinek
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The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni
Frontiers in Cellular and Infection Microbiology, 2018 Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms.
Muhammad A. B. Shabbir +11 more
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CRISPR/Cas9 in plant biotechnology: applications and challenges [PDF]
, 2022 The application of plant biotechnology to enhance beneficial traits in crops is now indispensable because of food insecurity due to increasing global population and climate change.
Gan, W.C. +3 more
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Current Opinion in Biotechnology, 2017 Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post ...
Richter, F. +5 more
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Optimizing CRISPR Cas9 Genome Editing System: A Review
International Journal of Endorsing Health Science Research, 2017 CRISPR Cas9 is highly advanced genome editing technology extensively used for the modifications of genetic components in various sectors of living organisms. This technology has been adapted from the prokaryotic immune system, where it plays a vital role
Meen Bahadur Budhthoki +5 more
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