Results 21 to 30 of about 266,379 (285)

Debating CRISPR/cas9 and Mitochondrial Donation: Continuity and Transition Performances at Scientific Conferences

Engaging Science, Technology, and Society, 2016
Conferences are important performative sites. Here we detail a UK science policy conference debating the novel biomedical techniques CRISPR/cas9 and mitochondrial donation.
Neil Stephens, Rebecca Dimond
doaj   +1 more source

Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Molecular & Cellular Oncology, 2020
We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines.
Veronica Rendo, Oana M. Enache, Uri Ben-David   +2 more
doaj   +1 more source

Creating a tobacco line with a weaker antifeedant property against colorado potato beetle

Биотехнология и селекция растений, 2020
Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina, A. V. Spaselnikova, A. A. Egorova, E. V. Kolosovskaya, D. V. Domrachev, A. V. Romanova, S. R. Tumanyan, S. Chamas, J. Kumlehn, I. M. Dubovskiy, S. V. Gerasimova   +10 more
doaj   +1 more source

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. [PDF]

, 2017
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase,
Bai, Haipeng, Huang, Haigen, Li, Song, Lin, Shuo, Lu, Xiaochan, Qin, Wei, Xu, Jason, Zhang, Yihan   +7 more
core   +1 more source

Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology

Zhongliu Fangzhi Yanjiu, 2020
Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells).
XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng   +3 more
doaj   +1 more source

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
core   +1 more source

Immunogenicity of Cas9 Protein [PDF]

Journal of Pharmaceutical Sciences, 2020
Clustered regularly interspaced short palindromic repeats (CRISPR) form the adaptive immune system in archaea and bacteria and have been modified for genome engineering in eukaryotic cells. CRISPR systems contain 2 components, a single-guide RNA, which is a short RNA composed of a 20 nucleotide sequence that targets specific sites in the genomic DNA ...
Mehta, Aditi, Merkel, Olivia M.
openaire   +2 more sources

Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA binding

Life Science Alliance, 2022
Cas9 is sumoylated and ubiquitylated in human cells. K848 is the major SUMO2/3 modification site, but multiple lysines are ubiquitylated, precipitating proteasomal degradation.
Tunahan Ergünay, Özgecan Ayhan, Arda B Celen, Panagiota Georgiadou, Emre Pekbilir, Yusuf T Abaci, Duygu Yesildag, Mandy Rettel, Ulduz Sobhiafshar, Anna Ogmen, NC Tolga Emre, Umut Sahin   +11 more
doaj   +1 more source

CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. [PDF]

, 2020
With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Chen, Qu, Fu, Xuemei, Kim, Jinchul, Liu, Jingfeng, Tang, Qingshuang, Xu, Shuxiang, Xu, Yang   +6 more
core  

Beyond seek and destroy: How to generate allelic series using genome editing tools [PDF]

, 2020
Genome editing tools have greatly facilitated the functional analysis of genes of interest by targeted mutagenesis. Many usable genome editing tools, including different site-specific nucleases and editor databases that allow single-nucleotide ...
Bes, Martine, Chaine, Christian, Durandet, Franz, Guiderdoni, Emmanuel, Herbert, Leo, Meunier, Anne Cecile, Michel, Rémy, Portefaix, Murielle, Périn, Christophe, This, Patrice, Vernet, Aurore   +10 more
core   +3 more sources