Results 21 to 30 of about 200,296 (309)

The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni

Frontiers in Cellular and Infection Microbiology, 2018
Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms.
Muhammad A. B. Shabbir, Yanping Tang, Zihui Xu, Mingyue Lin, Guyue Cheng, Menghong Dai, Xu Wang, Zhengli Liu, Zonghui Yuan, Zonghui Yuan, Haihong Hao, Haihong Hao   +11 more
doaj   +1 more source

Optimizing CRISPR Cas9 Genome Editing System: A Review

International Journal of Endorsing Health Science Research, 2017
CRISPR Cas9 is highly advanced genome editing technology extensively used for the modifications of genetic components in various sectors of living organisms. This technology has been adapted from the prokaryotic immune system, where it plays a vital role
Meen Bahadur Budhthoki, Puja sharma, Saima Khan, Saroj Singh, Fu Chao , Li Yong   +5 more
doaj   +1 more source

Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines

Adipocyte, 2021
Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-
Tomás Suchý, Isabell Kaczmarek, Tomislav Maricic, Christian Zieschang, Torsten Schöneberg, Doreen Thor, Ines Liebscher   +6 more
doaj   +1 more source

Debating CRISPR/cas9 and Mitochondrial Donation: Continuity and Transition Performances at Scientific Conferences

Engaging Science, Technology, and Society, 2016
Conferences are important performative sites. Here we detail a UK science policy conference debating the novel biomedical techniques CRISPR/cas9 and mitochondrial donation.
Neil Stephens, Rebecca Dimond
doaj   +1 more source

Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Molecular & Cellular Oncology, 2020
We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines.
Veronica Rendo, Oana M. Enache, Uri Ben-David   +2 more
doaj   +1 more source

Creating a tobacco line with a weaker antifeedant property against colorado potato beetle

Биотехнология и селекция растений, 2020
Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina, A. V. Spaselnikova, A. A. Egorova, E. V. Kolosovskaya, D. V. Domrachev, A. V. Romanova, S. R. Tumanyan, S. Chamas, J. Kumlehn, I. M. Dubovskiy, S. V. Gerasimova   +10 more
doaj   +1 more source

Advances in CRISPR/Cas9

BioMed Research International, 2022
CRISPR/Cas9 technology has become the most examined gene editing technology in recent years due to its simple design, yet low cost, high efficiency, and simple operation, which can also achieve simultaneous editing of multiple loci. It can also be carried out without using plasmids, saving lots of troubles caused by plasmids.
openaire   +2 more sources

Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology

Zhongliu Fangzhi Yanjiu, 2020
Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells).
XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng   +3 more
doaj   +1 more source

Cas9 Functionally Opens Chromatin. [PDF]

PLoS ONE, 2016
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler ...
Amira A Barkal, Sharanya Srinivasan, Tatsunori Hashimoto, David K Gifford, Richard I Sherwood   +4 more
doaj   +1 more source

CRISPR/Cas9 i rak

Veterinarska stanica, 2021
CRISPR/Cas9 postala je snažna metoda za unošenje promjena u genom brojnih organizama. Prvi put otkriven u bakterijama kao dio prilagodljivog imunološkog sustava, CRISPR/Cas9 i njegove modificirane verzije u širokoj su uporabi u inženjeringu genoma i aktivaciji ili suzbijanju ekspresije gena.
Mimoune, Nora, Bahouh, Mohamed Wail, Boukhechem, Said, Khelef, Djamel, Kaidi, Rachid, Baazizi, Ratiba   +5 more
openaire   +2 more sources