Results 11 to 20 of about 266,379 (285)
Current Opinion in Biotechnology, 2017 Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post ...
Richter, F. +5 more
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G3: Genes, Genomes, Genetics, 2020 Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis. Transcriptional interference occurs at a
Victoria Jorgensen +12 more
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Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]
, 2018 The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan +3 more
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The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni
Frontiers in Cellular and Infection Microbiology, 2018 Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms.
Muhammad A. B. Shabbir +11 more
doaj +1 more source
Optimizing CRISPR Cas9 Genome Editing System: A Review
International Journal of Endorsing Health Science Research, 2017 CRISPR Cas9 is highly advanced genome editing technology extensively used for the modifications of genetic components in various sectors of living organisms. This technology has been adapted from the prokaryotic immune system, where it plays a vital role
Meen Bahadur Budhthoki +5 more
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Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
Adipocyte, 2021 Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-
Tomás Suchý +6 more
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Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]
, 2020 Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu +2 more
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An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]
, 2018 The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J +20 more
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Veterinarska stanica, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated system (Cas) is a system that provides immunity to most prokaryoticorganisms against viral attacks and other foreign bodies. CRISPR systems consist of a scissor-like protein called Cas9 and a genetic GPS guide “The guide RNA”.
Mimoune, Nora +4 more
openaire +3 more sources