Results 11 to 20 of about 200,296 (309)
Veterinarska stanica, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated system (Cas) is a system that provides immunity to most prokaryoticorganisms against viral attacks and other foreign bodies. CRISPR systems consist of a scissor-like protein called Cas9 and a genetic GPS guide “The guide RNA”.
Mimoune, Nora +4 more
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Cas9-induced DNA breaks trigger antigenic variation.
, 2023 (A) The schematic shows two examples of VSGs with salient features highlighted; the N-terminal signal sequence, Cys residues (red), predicted N-glycosylation sites (black) and C-terminal GPI anchor signal. Length, in amino acids, and class (B3 or C2) are
Michele Tinti (75217) +4 more
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Journal of Oral Microbiology, 2022 Introduction Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems are RNA-mediated adaptive immune systems that actagainst invading genetic elements such as phages or plasmids.
Harumi Mizuki +3 more
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Smash and DASH with Cas9 [PDF]
Genome Biology, 2016 DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
Ramani, Vijay, Shendure, Jay
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Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]
, 2015 CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro +5 more
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G3: Genes, Genomes, Genetics, 2020 Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis. Transcriptional interference occurs at a
Victoria Jorgensen +12 more
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In Vitro Enzymology of Cas9 [PDF]
, 2014 Cas9 is a bacterial RNA-guided endonuclease that uses base pairing to recognize and cleave target DNAs with complementarity to the guide RNA. The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms.
Carolin Anders, Martin Jinek
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Cas9-triggered chain ablation of cas9 as a gene drive brake [PDF]
Nature Biotechnology, 2016 With the advent of clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) technology, researchers can construct gene drives that can bias the inheritance of edited alleles to alter entire populations. As demonstrated with the mutagenic chain reaction in Drosophila4, the CRISPR-Cas9 system can propagate ...
Wu, Bing, Luo, Liqun, Gao, Xiaojing J.
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Defining the Target DNA Cut in CRISPR-Cas9
, 2022 Structures of reactants, products and intermediate states during DNA cleavage in the CRISPR-Cas9 HNH nuclease. Data are provided in PDB format. Two VMD states are also provided to facilitate visualization through the Visual Molecular Dynamics (VMD ...
Mohd Ahsan (7301282) +9 more
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Current Opinion in Biotechnology, 2017 Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post ...
Richter, F. +5 more
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