Results 11 to 20 of about 228,880 (307)
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
World Cancer Research Journal, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
M. Niuz Morshed Khan +3 more
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Gene Editing to Tackle Facioscapulohumeral Muscular Dystrophy
Frontiers in Genome Editing, 2022 Facioscapulohumeral dystrophy (FSHD) is a skeletal muscle disease caused by the aberrant expression of the DUX4 gene in the muscle tissue. To date, different therapeutic approaches have been proposed, targeting DUX4 at the DNA, RNA or protein levels. The
Virginie Mariot, Julie Dumonceaux
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Immunogenicity of Cas9 Protein [PDF]
Journal of Pharmaceutical Sciences, 2020 Clustered regularly interspaced short palindromic repeats (CRISPR) form the adaptive immune system in archaea and bacteria and have been modified for genome engineering in eukaryotic cells. CRISPR systems contain 2 components, a single-guide RNA, which is a short RNA composed of a 20 nucleotide sequence that targets specific sites in the genomic DNA ...
Mehta, Aditi, Merkel, Olivia M.
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Entering the post-epigenomic age: back to epigenetics [PDF]
Open Biology, 2018 It is undeniably one of the greatest findings in biology that (with some very minor exceptions) every cell in the body possesses the whole genetic information needed to generate a complete individual.
Sebastian Bultmann, Stefan H. Stricker
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Multiplexable and Biocomputational Virus Detection by CRISPR-Cas9-Mediated Strand Displacement
, 2023 Recurrent disease outbreaks caused by different viruses, including the novel respiratory virus SARS-CoV-2, are challenging our society at a global scale; so versatile virus detection methods would enable a calculated and faster response. Here, we present
Roser Montagud-Martínez (6730088) +7 more
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Two-Metal Ion Mechanism of DNA Cleavage in CRISPR-Cas9 [PDF]
, 2019 CRISPR-Cas9 is a cutting-edge genome-editing technology, which employs the endonuclease Cas9 to cleave DNA sequences of interest. However, the catalytic mechanism of DNA cleavage and the critical role of the Mg2+ ions have remained elusive. Here, quantum–
Martin Jinek (7351819) +2 more
core +2 more sources
Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases
Acta Biomedica Scientifica, 2023 The aim. To analyze the possibility of using the genetic mechanisms of CRISPR-Cas9 technology in the prevention and treatment of certain viral diseases.Materials and methods.
A. M. Ziganshin +4 more
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CRISPR/CAS9 Technologies [PDF]
Journal of Bone and Mineral Research, 2017 ABSTRACT The Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) pathway is revolutionizing biological research. Modifications to this primitive prokaryotic immune system now enable scientists to efficiently edit DNA or modulate gene expression in living ...
Bart O, Williams, Matthew L, Warman
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Cas9-induced DNA breaks trigger antigenic variation.
, 2023 (A) The schematic shows two examples of VSGs with salient features highlighted; the N-terminal signal sequence, Cys residues (red), predicted N-glycosylation sites (black) and C-terminal GPI anchor signal. Length, in amino acids, and class (B3 or C2) are
Michele Tinti (75217) +4 more
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P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri [PDF]
, 2023 The discovery of clustered, regularly interspaced, short palindromic repeats (CRISPR) and the Cas9 RNA-guided nuclease provides unprecedented opportunities to selectively kill specific populations or species of bacteria.
Serge Mostowy +7 more
core +3 more sources