Results 31 to 40 of about 228,880 (307)
Adipocyte, 2021 Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-
Tomás Suchý +6 more
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Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Theranostics, 2021 CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy has been hindered by efficient delivery of CRISPR/Cas9 materials into target cells. Direct delivery of CRISPR/Cas9 system as a ribonucleoprotein (RNP) complex consisting of Cas9 protein and single guide RNA ...
Zhang, Song +3 more
openaire +2 more sources
Defining the Target DNA Cut in CRISPR-Cas9
, 2022 Structures of reactants, products and intermediate states during DNA cleavage in the CRISPR-Cas9 HNH nuclease. Data are provided in PDB format. Two VMD states are also provided to facilitate visualization through the Visual Molecular Dynamics (VMD ...
Mohd Ahsan (7301282) +9 more
core +1 more source
Engaging Science, Technology, and Society, 2016 Conferences are important performative sites. Here we detail a UK science policy conference debating the novel biomedical techniques CRISPR/cas9 and mitochondrial donation.
Neil Stephens, Rebecca Dimond
doaj +1 more source
Adding to the CASeload: unwarranted p53 signaling induced by Cas9
Molecular & Cellular Oncology, 2020 We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines.
Veronica Rendo +2 more
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Creating a tobacco line with a weaker antifeedant property against colorado potato beetle
Биотехнология и селекция растений, 2020 Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina +10 more
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Veterinarska stanica, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated system (Cas) is a system that provides immunity to most prokaryoticorganisms against viral attacks and other foreign bodies. CRISPR systems consist of a scissor-like protein called Cas9 and a genetic GPS guide “The guide RNA”.
Mimoune, Nora +4 more
openaire +3 more sources
Identification and Evolution of Cas9 tracrRNAs [PDF]
The CRISPR Journal, 2020 Clustered regularly interspaced palindromic repeats (CRISPR)-associated (Cas)9 transactivating CRISPR RNAs (tracrRNAs) form distinct structures essential for target recognition and cleavage and dictate exchangeability between orthologous proteins. As noncoding RNAs that are often apart from the CRISPR array, their identification can be arduous. In this
Shane K. Dooley +4 more
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, 2023 Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203) +6 more
core +1 more source
Cas9 Functionally Opens Chromatin. [PDF]
PLoS ONE, 2016 Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler ...
Amira A Barkal +4 more
doaj +1 more source