Results 31 to 40 of about 200,296 (309)

Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA binding

Life Science Alliance, 2022
Cas9 is sumoylated and ubiquitylated in human cells. K848 is the major SUMO2/3 modification site, but multiple lysines are ubiquitylated, precipitating proteasomal degradation.
Tunahan Ergünay, Özgecan Ayhan, Arda B Celen, Panagiota Georgiadou, Emre Pekbilir, Yusuf T Abaci, Duygu Yesildag, Mandy Rettel, Ulduz Sobhiafshar, Anna Ogmen, NC Tolga Emre, Umut Sahin   +11 more
doaj   +1 more source

Computationally designed hyperactive Cas9 enzymes

Nature Communications, 2022
The ability to alter the genomes of living cells is key to understanding how genes influence the functions of organisms and will be critical to modify living systems for useful purposes. Here, the authors use computational design to discover Cas9 enzymes
Pascal D. Vos, Giulia Rossetti, Jessica L. Mantegna, Stefan J. Siira, Andrianto P. Gandadireja, Mitchell Bruce, Samuel A. Raven, Olga Khersonsky, Sarel J. Fleishman, Aleksandra Filipovska, Oliver Rackham   +10 more
doaj   +1 more source

Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination

, 2021
Genetic engineering of industrial cell lines often requires knockout of multiple endogenous genes. Tools like CRISPR-Cas9 have enabled serial or parallelized gene disruption in a wide range of industrial organisms, but common practices for the screening ...
Neil C. Dalvie (3287133), Timothy Lorgeree, Timothy Lorgeree (11820308), Andrew M. Biedermann, Kerry R. Love (4490911), Neil C. Dalvie, Andrew M. Biedermann (10735208), Kerry R. Love, J. Christopher Love, J. Christopher Love (159667)   +9 more
core   +1 more source

Two-Metal Ion Mechanism of DNA Cleavage in CRISPR-Cas9

, 2019
CRISPR-Cas9 is a cutting-edge genome-editing technology, which employs the endonuclease Cas9 to cleave DNA sequences of interest. However, the catalytic mechanism of DNA cleavage and the critical role of the Mg2+ ions have remained elusive. Here, quantum–
Martin Jinek (7351819), Giulia Palermo (6168377), Lorenzo Casalino (7351818)   +2 more
core   +2 more sources

Delivering CRISPR: a review of the challenges and approaches

Drug Delivery, 2018
Gene therapy has long held promise to correct a variety of human diseases and defects. Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the mechanism of the CRISPR-based prokaryotic adaptive immune system (CRISPR ...
Christopher A. Lino, Jason C. Harper, James P. Carney, Jerilyn A. Timlin   +3 more
doaj   +1 more source

Off- and on-target effects of genome editing in mouse embryos

The Journal of Reproduction and Development, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE, Kenichi NAKASHIMA, Atsushi YOSHIKI   +2 more
doaj   +1 more source

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells

Genes and Diseases, 2022
The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to ...
Renke Tan, Wenhao Du, Yiyang Liu, Xiaoji Cong, Meirong Bai, Chenxiao Jiang, Zengxia Li, Minjia Tan, Dengke K. Ma, Qiang Huang, Wei Jiang, Yongjun Dang   +11 more
doaj   +1 more source

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

Table3_Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits.docx

, 2023
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications.
Nick Strelchenko (14392203), Jenna Kropp Schmidt (14392200), Sarah R. Gierczic (14392206), Thaddeus G. Golos (3375128), Igor I. Slukvin (14392212), Derek Pavelec (14392209), Yun Hee Kim (12511708)   +6 more
core   +1 more source

Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9

, 2014
This work was supported in part by NIH grant 1DP5OD017892 to S.LToxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the
Saima M Sidik, Sebastian Lourido, Caroline G. Hackett (226300), Hackett, Caroline G., Lourido, Sebastian, Caroline G Hackett, Saima M. Sidik (590022), Nicholas J Westwood, Nicholas J. Westwood (254891), Sidik, Saima M., Sebastian Lourido (590023), Fanny Tran, Tran, Fanny, Westwood, Nicholas J., Fanny Tran (571869)   +14 more
core   +1 more source