Results 81 to 90 of about 200,296 (309)

High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation

Cell Reports, 2020
Summary: A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9
Mateusz Legut, Zharko Daniloski, Xinhe Xue, Dayna McKenzie, Xinyi Guo, Hans-Hermann Wessels, Neville E. Sanjana   +6 more
doaj   +1 more source

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. [PDF]

PLoS ONE, 2015
The type II clustered regularly interspaced short palindromic repeats (CRISPR) associated with Cas9 endonuclease (CRISPR/Cas9) has become a powerful genetic tool for understanding the function of a gene of interest.
Hirohito Kotani, Kiyohito Taimatsu, Rie Ohga, Satoshi Ota, Atsuo Kawahara   +4 more
doaj   +1 more source

MOESM1 of CRISPR-Cas9-mediated knockout of SPRY2 in human hepatocytes leads to increased glucose uptake and lipid droplet accumulation

, 2019
Additional file 1: Supplementary Methods. Figure S1. Overview of the SPRY2 locus. Figure S2. Exon organisation of SPRY2 gene. Figure S3. Examples of DNA mutations generated following CRISPR-Cas9 genome editing for SPRY2 in HepG2 cells. Figure S4.
Abhiram Rao (7587503), Casimiro Castillejo-Lรณpez (7587512), Erik Ingelsson (232651), Susanne Hetty (7587506), Milos Pjanic (485288), Naomi Cook (4738857), Thomas Quertermous (94604), Joshua Knowles (116765), Andrew Emmerich (7587500)   +8 more
core   +1 more source

Additional file 1 of CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition

, 2020
Additional file 1. gRNA sequences and potential off-target loci: Off-target sites as identified by Integrated DNA Technologies’ CRISPR-Cas9 gRNA Design Checker with mismatches (#MM) threshold set to 3.
L. Shaw (6427883), R. Williams (3511298), K. Hamill (8480826)   +2 more
core   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Rice, 2020
Background Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes.
Raviraj Banakar, Mollie Schubert, Michael Collingwood, Christopher Vakulskas, Alan L. Eggenberger, Kan Wang   +5 more
doaj   +1 more source

Investigating the role of chromatin modifications in CRISPR/Cas9 gene editing [PDF]

, 2020
Precisely positioned nucleosomes and heterochromatin have been shown to impede CRISPR/Cas9 editing efficiency. Conversely, Cas9 can open previously inaccessible regions of DNA, and transcriptionally silent targets can usually be edited without ...
Kallimasioti Pazi, Eirini Margarita
core   +1 more source

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways.

, 2023
Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by modulating DNA repair pathways holds considerable promise to increase the ...
Sven van der Vlies (14520668), Juliette A Kamp (14520671), Steven A Kushner (14520677), Núria Muñoz-Subirana (14520662), Hanneke Kool (14520665), Marcel Tijsterman (155541), Joost Schimmel (11052642), Robin van Schendel (3226518), Femke de Vrij (14520674), Graeme CM Smith (11052684), Simon J Boulton (8274444)   +10 more
core   +1 more source