Results 81 to 90 of about 365,538 (313)

Exploring the Antimicrobial Potential of a Novel Phage-Derived Lytic Protein Against Pseudomonas aeruginosa

Current Issues in Molecular Biology
The escalation of bacterial resistance to existing antibiotics represents a growing global health challenge, exacerbated by the widespread misuse of antimicrobial agents.
Sibongile Mtimka, Kanyane Bridgett Malatji, Patrick Opare Sakyi, Noel David Nogbou, Andrew Munyalo Musyoki, Sipho Mamputha, Lusisizwe Kwezi, Samuel Kojo Kwofie, Ofentse Jacob Pooe, Tsepo Lebiletsa Tsekoa   +9 more
doaj   +1 more source

The Second Catalytic Domain of Protein Tyrosine Phosphatase δ (PTPδ) Binds to and Inhibits the First Catalytic Domain of PTPς [PDF]

Molecular and Cellular Biology, 1998
The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP sigma (PTP sigma-D1) as bait to ...
M J, Wallace, C, Fladd, J, Batt, D, Rotin   +3 more
openaire   +2 more sources

The activation mechanism of Irga6, an interferon-inducible GTPase contributing to mouse resistance against Toxoplasma gondii [PDF]

, 2011
Background: The interferon-inducible immunity-related GTPases (IRG proteins/p47 GTPases) are a distinctive family of GTPases that function as powerful cell-autonomous resistance factors.
Khaminets, Aliaksandr, Wolf, Eva, Vopper, G., Pawlowski, Nikolaus, Schmidt, Andreas, Lange Rita, Aliaksandr Khaminets, Nikolaus Pawlowski, Papic, Natasa, Gabriela Vopper, Papic Natasa, Pawlowski, N., Uthaiah, Revathy C., Natasa Papic, Howard, J. C., Hunn, Julia P., Uthaiah, R. C., Andreas Schmidt, Eva Wolf, Hunn, J., Vopper, Gabriela, Julia P Hunn, Schmidt Andreas, Rita Lange, Sascha Martens, Uthaiah, Revathy Chottekalapanda, Martens Sascha, Wolf, E., Jonathan C Howard, Lange, Rita, Martens, Sascha, Papic, N., Wolf Eva, Lange, R., Hunn Julia P, Khaminets Aliaksandr, Howard, Jonathan C., Howard, J., Howard Jonathan C, Schmidt, A., Uthaiah Revathy C, Khaminets, A., Pawlowski Nikolaus, Revathy C Uthaiah, Uthaiah, R., Martens, S., Hunn, J. P., Hunn, Julia, Vopper Gabriela   +48 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

β‐Helical catalytic domains in glycoside hydrolase families 49, 55 and 87: domain architecture, modelling and assignment of catalytic residues [PDF]

FEBS Letters, 2002
X‐ray crystallography and bioinformatics studies reveal a tendency for the right‐handed β‐helix domain architecture to be associated with carbohydrate binding proteins. Here we demonstrate the presence of catalytic β‐helix domains in glycoside hydrolase (GH) families 49, 55 and 87 and provide evidence for their sharing a common evolutionary ancestor ...
Rigden, Daniel J, Franco, Octávio L
openaire   +2 more sources

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Dynamic Allostery Modulates Catalytic Activity by Modifying the Hydrogen Bonding Network in the Catalytic Site of Human Pin1

Molecules, 2017
Allosteric communication among domains in modular proteins consisting of flexibly linked domains with complimentary roles remains poorly understood. To understand how complementary domains communicate, we have studied human Pin1, a representative modular
Jing Wang, Ryosuke Kawasaki, Jun-ichi Uewaki, Arif U. R. Rashid, Naoya Tochio, Shin-ichi Tate   +5 more
doaj   +1 more source

Phosphorylation in the Catalytic Cleft Stabilizes and Attracts Domains of a Phosphohexomutase [PDF]

Biophysical Journal, 2015
Phosphorylation can modulate the activities of enzymes. The phosphoryl donor in the catalytic cleft of α-D-phosphohexomutases is transiently dephosphorylated while the reaction intermediate completes a 180° reorientation within the cleft. The phosphorylated form of 52 kDa bacterial phosphomannomutase/phosphoglucomutase is less accessible to dye or ...
Xu, Jia, Lee, Yingying, Beamer, Lesa J., Van Doren, Steven R.   +3 more
openaire   +2 more sources

Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator

, 2014
Diguanylate cyclases (DGC) and phosphodiesterases (PDE) respectively synthesise and hydrolyse the secondary messenger cyclic dimeric GMP (c-di-GMP), and both activities are often found in a single protein.
Mikolajek, Halina, Keevil, Charles William, Schlaefli, Henry George, Webb, Jeremy Stephen, Tews, Ivo, Phippen, Curtis William   +5 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source