Results 61 to 70 of about 574,974 (306)

Functional analysis of the N‐terminal region of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis

FEBS Open Bio, 2022
Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis (TTE0866) has an N‐terminal region (NTR; residues 23–135) between the signal sequence (residues 1–22) and the catalytic domain (residues 136–324), which is of unknown function ...
Kohei Sasamoto, Tomoki Himiyama, Kunihiko Moriyoshi, Takashi Ohmoto, Koichi Uegaki, Tsutomu Nakamura, Yoshiaki Nishiya   +6 more
doaj   +1 more source

Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes [PDF]

, 2015
Until relatively recently, DNA primases were viewed simply as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replication.
Aidan J. Doherty, Benjamin A. Keen, Bianchi, Bouché, Collins, Crute, Kaguni, Klinedinst, Klinedinst, Koonin, Marsden, Moss, Nigel C. Brissett, Rowen, Scherzinger, Sherman, Takechi, Thomas A. Guilliam   +17 more
core   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

A bifunctional endolytic alginate lyase with two different lyase catalytic domains from Vibrio sp. H204

Frontiers in Microbiology
Alginate lyases can fully degrade alginate into various size-defined unsaturated oligosaccharide products by β-elimination. Here, we identified the bifunctional endolytic alginate lyase Aly35 from the marine bacterium Vibrio sp. Strain H204.
Chune Peng, Qingbin Wang, Qingbin Wang, Wei Xu, Xinkun Wang, Qianqian Zheng, Xiaohui Liang, Xiaodan Dong, Fuchuan Li, Lizeng Peng   +9 more
doaj   +1 more source

Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56

Nature Communications, 2023
Bacterial AB toxins are secreted key virulence factors that are internalized by target cells through receptor-mediated endocytosis, translocating their enzymatic domain to the cytosol from endosomes (short-trip) or the endoplasmic reticulum (long-trip ...
Johnny Lisboa, Cassilda Pereira, Rute D. Pinto, Inês S. Rodrigues, Liliana M. G. Pereira, Bruno Pinheiro, Pedro Oliveira, Pedro José Barbosa Pereira, Jorge E. Azevedo, Dominique Durand, Roland Benz, Ana do Vale, Nuno M. S. dos Santos   +12 more
doaj   +1 more source

The catalytic core of DEMETER guides active DNA demethylation in Arabidopsis. [PDF]

, 2019
The Arabidopsis DEMETER (DME) DNA glycosylase demethylates the maternal genome in the central cell prior to fertilization and is essential for seed viability.
Aravind, L., Fischer, Robert L., Frost, Jennifer M., Hsieh, Tzung-Fu, Huh, Jin Hoe, Hung, Yu-Hung, Iyer, Lakshminarayan M., Jang, Hosung, Liu, Fang, Rim, Hyun Jung, Shin, Hosub, Xiao, Wenyan, Zhang, Changqing, Zhang, Dapeng, Zhang, Xiang-Qian, 허진회   +15 more
core   +2 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Kinetic Analysis of the Catalytic Domain of Human Cdc25B [PDF]

Journal of Biological Chemistry, 1996
The Cdc25 cell cycle regulator is a member of the dual-specificity class of protein-tyrosine phosphatases that hydrolyze phosphotyrosine- and phosphothreonine-containing substrates. To study the mechanism of Cdc25B, we have overexpressed and purified the catalytic domain of human Cdc25B (Xu, X., and Burke, S. P. (1996) J. Biol. Chem.
E B, Gottlin, X, Xu, D M, Epstein, S P, Burke, J W, Eckstein, D P, Ballou, J E, Dixon   +6 more
openaire   +2 more sources

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

High coenzyme affinity chimeric amine dehydrogenase based on domain engineering

Bioresources and Bioprocessing, 2022
NADH-dependent phenylalanine amine dehydrogenase (F-AmDH) engineered from phenylalanine dehydrogenase (PheDH) catalyzes the synthesis of aromatic chiral amines from prochiral ketone substrates.
Jialin Li, Xiaoqing Mu, Tao Wu, Yan Xu
doaj   +1 more source