Results 61 to 70 of about 5,363 (277)

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Systems Thinking with Causal Loop Diagrams in Medical Education: An Exploratory Study

Systems
Medical literature is replete with diagrammatic representations of systems, yet lacks standardised nomenclature and consistent symbolic conventions. In an introductory system dynamics course for health science students, causal loop diagrams (CLDs) are ...
David M. Rubin, Shamin Achari, Xriz L. Richards, Adam Pantanowitz, Ann George   +4 more
doaj   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Group model building with causal loop diagrams to foster capabilities for sustainable design and product development

, 2020
This research proposes a group model building workshop method that uses causal loop diagrams to foster capabilities for sustainable product development based on feedback and observations from several cases.
Watz, Matilda,, Watz, Matilda, Hallstedt, Sophie,, Hallstedt, Sophie   +3 more
core   +1 more source

Understanding ecosystem service impacts of tidal wetlands after managed dyke realignment through causal loop diagrams

, 2023
This report explores ecological dynamics involved in the provision of three ecosystem services (blue carbon, storm protection, and fisheries) associated with existing and restored tidal wetlands in the context of managed dyke realignment.
Cornejo, Lara, James, Patrick
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Learning Together for Groundwater Management: A Case of the Devnadi Basin, Nashik, Maharashtra, India

International Journal of the Commons
This paper documents the process of co-learning spaces for transdisciplinary learning for participatory groundwater and ecosystem management across research-practice-community collectives in the Devnadi River Basin, Sinnar Block of Nashik District in ...
Shuchi Vora
doaj   +1 more source

Participatory systems science for enhancing health and wellbeing in the Indian Ocean territories

Frontiers in Public Health, 2023
ObjectivesCo-creation of diabetes and obesity prevention with remote communities allows local contextual factors to be included in the design, delivery, and evaluation of disease prevention efforts.
Steven Allender, Syarifah Liza Munira, Siobhan Bourke, Emily Lancsar   +3 more
doaj   +1 more source