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Hematopoietic stem cells: Are they CD34-positive or CD34-negative?
Nature Medicine, 1998New findings add to the growing evidence that some stem cells in the bone marrow do not express the CD34 marker, which is currently used to select stem cells for transplantation ( pages 1038–1045 ).
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Evaluation of Human and Rhesus CD34+CXCR4+ and CD34+CXCR4- Subpopulations
Blood, 2011Abstract Abstract 1007 CXCR4 is an α-chemokine receptor specific for the ligand stromal-derived-factor-1 (SDF-1). CXCR4 is expressed on several cell types, including CD34+ cells. In this study, we sought to evaluate the difference between subpopulations of human and rhesus CD34+ cells that express CXCR4. Rhesus CD34+ cells
Naoya Uchida +7 more
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CD34 Immunophenotyping of Blasts in Myelodysplasia
Leukemia & Lymphoma, 1994Immunological analysis of bone marrow cells in myelodysplasia using immunofluorescence did not allow accurate morphological identification of blast cells. However, improvement of the immunoperoxidase technique allows one to realize the diagnostic potential of immunocytochemistry.
J, Oertel, D, Huhn
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CD34+ Leukemic Cells Assessed by Different CD:34 Monoclonal Antibodies
Leukemia & Lymphoma, 1995CD34 monoclonal antibodies (McAbs) are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias. We assessed the reactivity of 17 CD34 McAbs from the 5th International Workshop on Leukocyte Differentiation Antigens with a variety of cells types: normal bone marrow hemopoietic progenitors, 10 AML, 6 ALL, 11
LANZA, Francesco +6 more
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CD34 measurement: setting standards
Cytotherapy, 2014Flow cytometric analysis has provided the hematopoietic transplant community with a powerful and rapid tool for assessing the engraftment potential of stem cell products. As described by Preti et al. [1] in this issue of the Journal, this was made possible by the identification of the CD34 antigen, and the commercial availability of fluorescently ...
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Transmigration of Human CD34+ Cells
2008Understanding mechanisms responsible for engraftment of hematopoietic stem cells (HSC) is important to achieve successful HSC transplantation. Homing of HSC to the bone marrow niche is believed to be a crucial step for engraftment. However, the molecular mechanisms that regulate HSC homing are not understood well.
Seiji, Fukuda, Louis M, Pelus
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Cytotherapy, 2012
Today ’ s rapid development of new technologies, tools and reagents leaves many of us in search of reliable and detailed information about these new products. Product brochures provided by manufacturers and distributors often lack key information about performance and quality, and rarely include ...
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Today ’ s rapid development of new technologies, tools and reagents leaves many of us in search of reliable and detailed information about these new products. Product brochures provided by manufacturers and distributors often lack key information about performance and quality, and rarely include ...
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Who is hematopoietic stem cell: CD34+ or CD34-?
International journal of hematology, 2000Hematopoietic stem cells (HSC) possess multipotentiality, enabling them to self-renew and also to produce mature blood cells, such as erythrocytes, leukocytes, platelets, and lymphocytes. CD34 is a marker of human HSC, and all colony-forming activity of human bone marrow (BM) cells is found in the CD34+ fraction.
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2019
Antibodies against cell-surface proteins play an important role in cell detection, separation and determination of differentiation stage. The globular structure of extracellular region of cell-surface antigens is frequently characterized by heavily glycosilation and/or is stabilized by disulphide bonds.
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Antibodies against cell-surface proteins play an important role in cell detection, separation and determination of differentiation stage. The globular structure of extracellular region of cell-surface antigens is frequently characterized by heavily glycosilation and/or is stabilized by disulphide bonds.
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