Results 161 to 170 of about 22,951 (177)

ets-2 Regulates cdc2 Kinase Activity in Mammalian Cells: Coordinated Expression of cdc2 and Cyclin A

Experimental Cell Research, 1995
ets-2 is a member of a family of transcription factors implicated in the regulation of gene expression during cell proliferation, cell differentiation, and development. We report that the ets-2 protein transactivates the promoter of the cdc2 gene which encodes a 34-kDa serine-threonine kinase required for mitotic initiation in mammalian cells ...
Wen SC, Ku DH, De Luca A, Claudio PP, Giordano A, CALABRETTA, Bruno   +5 more
openaire   +6 more sources

Beacon interacts with cdc2/cdc28-like kinases

Biochemical and Biophysical Research Communications, 2003
Previously we found elevated beacon gene expression in the hypothalamus of obese Psammomys obesus. Beacon administration into the lateral ventricle of P. obesus stimulated food intake and body weight gain. In the current study we used yeast two-hybrid technology to screen for proteins in the human brain that interact with beacon.
Lakshmi, Kantham, Lyndal, Kerr-Bayles, Nathan, Godde, Melissa, Quick, Ryan, Webb, Terry, Sunderland, Judy, Bond, Ken, Walder, Guy, Augert, Greg, Collier   +9 more
openaire   +2 more sources

Regulatory properties of neuronal cdc2-like kinase

Molecular and Cellular Biochemistry, 1995
Neuronal cdc2-like kinase, nclk, is a heterodimer of cyclin dependent protein kinase 5, cdk5, and a 25 kDa subunit derived from a novel, neuron-specific, 35 kDa protein: p35. The characterization and regulation of nclk will be summarized in this minireview.
Qi, Robert Zhong, Yang, D., Matsuura, I., Lee, K.Y., Zhu, X., Huang, Q.Q., Wang, J.H.   +6 more
openaire   +2 more sources

Entry into mitosis without Cdc2 kinase activation

Journal of Cell Science, 1998
ABSTRACT Mouse FT210 cells at 39°C cannot enter mitosis but arrest in G2 phase, because they lack Cdc2 kinase activity as a result of a temperature-sensitive lesion in the cdc2 gene. Incubation of arrested cells with the protein phosphatase 1 and 2A inhibitor okadaic acid induces morphologically normal chromosome condensation.
Gowdy, P. M., Anderson, H. J., Roberge, M.   +2 more
openaire   +2 more sources

Genetic analysis of the Drosophila cdc2 homolog

Development, 1993
ABSTRACT We have identified mutations in the Drosophila cdc2 gene. The recessive lethality of these mutant alleles was rescued after P-element-mediated transformation with a genomic cdc2 fragment. Sequence analysis of amorphic alleles revealed non-conservative exchanges in evolutionary conserved positions.
Stern, B., Ried, G., Clegg, N., Grigliatti, T., Lehner, C.   +4 more
openaire   +3 more sources

Phosphorylation of Synthetic Vimentin Peptides by CDC2 Kinase

Biochemical and Biophysical Research Communications, 1993
Synthetic peptide representing the site Ser-41 in vimentin, Leu-Gly-Ser41-Ala42-Leu-Arg44-Arg-Arg-NH2, and its analogs in which Ala-42 was replaced by various amino acids were tested as substrates for cdc2 kinase. Among them, the analog containing sarcosine as well as proline was an excellent substrate.
S, Ando, K, Tsujimura, Y, Matsuoka, T, Tokui, S, Hisanaga, E, Okumura, M, Uchiyama, T, Kishimoto, H, Yasuda, M, Inagaki   +9 more
openaire   +2 more sources

Demonstration of cdc2 kinase in mature neuronal nuclei

NeuroReport, 1993
Immunocytochemistry has been used to assess the distribution of the mitosis-associated protein kinase, cdc2, in mature neurons of rat brain. A robust signal is apparent in most, but not all neurons in cerebellum, cortex and hippocampus. The signal is concentrated in neuronal nuclei with lower levels of immunoreactivity in neuronal cytoplasm.
J G, Wood, P, Zinsmeister
openaire   +2 more sources

The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function

Molecular and General Genetics MGG, 1986
A DNA fragment called suc1 has been found to rescue cells mutated in the cell cycle control gene cdc2 of the fission yeast Schizosaccharomyces pombe. The suppressing activity of suc1 is observed when it is present on a multicopy number plasmid. The gene does not hybridize to cdc2 and maps elsewhere in the genome.
J, Hayles, D, Beach, B, Durkacz, P, Nurse   +3 more
openaire   +2 more sources

Activation of the various cyclin/cdc2 protein kinases

Current Opinion in Cell Biology, 1993
Recent research has led to the near culmination of the biochemical confirmation of the most basic genetic predictions about how p34cdc2 activity is controlled. The field is now moving from a biochemical dissection of the machinery to understanding how the system is regulated.
openaire   +2 more sources

cdc2 protein kinase: structure-function relationships.

Ciba Foundation symposium, 1993
Activation of the cdc2 kinase in the cell cycle occurs upon binding to a regulatory subunit called cyclin. Cyclin A associates with both Cdc2 and its homologue Cdk2. The two complexes appear in S phase but cyclin A/Cdk2 is activated earlier than cyclin A/Cdc2. Several regions in Cdc2 are involved in binding cyclins A and B.
M J, Marcote, M, Pagano, G, Draetta
openaire   +1 more source