Results 31 to 40 of about 25,474 (119)

Glucose- and GTP-dependent stimulation of the carboxyl methylation of CDC42 in rodent and human pancreatic islets and pure beta cells. Evidence for an essential role of GTP-binding proteins in nutrient-induced insulin secretion. [PDF]

Journal of Clinical Investigation, 1996
Several GTP-binding proteins (G-proteins) undergo post-translational modifications (isoprenylation and carboxyl methylation) in pancreatic beta cells. Herein, two of these were identified as CDC42 and rap 1, using Western blotting and immunoprecipitation.
Mary E. Rabaglia, Mary E. Rabaglia, Robert L. Sorenson, Stewart A. Metz, Stewart A. Metz, Rafael Nesher, Anjaneyulu Kowluru, Anthony J. Weinhaus, Jacob Vadakekalam, Jacob Vadakekalam, Scott E. Seavey, Scott E. Seavey, Guodong Li, Guodong Li   +13 more
openaire   +3 more sources

Detecting Protein-Protein Interactions Using Renilla Luciferase Fusion Proteins

BioTechniques, 2002
We have developed a novel system designated the luciferase assay for protein detection (LAPD) to study protein-protein interactions. This method involves two protein fusions, a soluble reporter fusion and a fusion for immobilizing the target protein. The
Peter D. Burbelo, Adam E. Kisailus, Jeremy W. Peck   +2 more
doaj   +1 more source

Pattern localization to a domain edge [PDF]

Phys. Rev. E 101, 022414 (2020), 2019
The formation of protein patterns inside cells is generically described by reaction-diffusion models. The study of such systems goes back to Turing, who showed how patterns can emerge from a homogenous steady state when two reactive components have different diffusivities (e.g. membrane-bound and cytosolic states). However, in nature, systems typically
arxiv   +1 more source

Suppl. Fig. S4 from Characterization of Novel Derivatives of MBQ-167, an Inhibitor of the GTP-binding Proteins Rac/Cdc42

, 2023
<p>Supplementary Figure S4 shows the safety of MBQ compounds in mice.</p>
Suranganie Dharmawardhane, Cornelis P. Vlaar, Eliud Hernandez O'Farril, Marcelo G. Kazanietz, Mariana Cooke, Luis D. Borrero-Garcia, Tatiana Matos Gascot, Maria del Mar Maldonado, Ailed Cruz-Collazo, Julia I. Medina   +9 more
openaire   +1 more source

Host Defense Peptide LL-37-Mediated Chemoattractant Properties, but Not Anti-Inflammatory Cytokine IL-1RA Production, Is Selectively Controlled by Cdc42 Rho GTPase via G Protein-Coupled Receptors and JNK Mitogen-Activated Protein Kinase

Frontiers in Immunology, 2018
The human host defense peptide LL-37 promotes immune activation such as induction of chemokine production and recruitment of leukocytes. Conversely, LL-37 also mediates anti-inflammatory responses such as production of anti-inflammatory cytokines, e.g ...
Mahadevappa Hemshekhar, Ka-Yee Grace Choi, Ka-Yee Grace Choi, Neeloffer Mookherjee, Neeloffer Mookherjee   +4 more
doaj   +1 more source

Pattern Formation in a Coupled Membrane-Bulk Reaction-Diffusion Model for Intracellular Polarization and Oscillations [PDF]

Journal of Theoretical Biology, 2020, Vol. 497, 110242 (23 pages), 2019
Reaction-diffusion systems have been widely used to study spatio-temporal phenomena in cell biology, such as cell polarization. Coupled bulk-surface models naturally include compartmentalization of cytosolic and membrane-bound polarity molecules. Here we study the distribution of the polarity protein Cdc42 in a mass-conserved membrane-bulk model, and ...
arxiv   +1 more source

The small GTP-binding proteins Rac1 and Cdc42regulate the activity of the JNK/SAPK signaling pathway

Cell, 1995
c-Jun amino-terminal kinases (JNKs) and mitogen-activated protein kinases (MAPKs) are closely related; however, they are independently regulated by a variety of environmental stimuli. Although molecules linking growth factor receptors to MAPKs have been recently identified, little is known about pathways controlling JNK activation.
Coso OA, Chiariello M, Yu, JC, Teramoto H, Crespo P, Xu N, Miki T, Gutkind JS   +8 more
openaire   +4 more sources

Suppl. Fig. S3 from Characterization of Novel Derivatives of MBQ-167, an Inhibitor of the GTP-binding Proteins Rac/Cdc42

, 2023
<p>Suppl. Fig. S3 shows the effect of MBQ-167 and derivatives on lung cancer cells.</p>
Suranganie Dharmawardhane, Cornelis P. Vlaar, Eliud Hernandez O'Farril, Marcelo G. Kazanietz, Mariana Cooke, Luis D. Borrero-Garcia, Tatiana Matos Gascot, Maria del Mar Maldonado, Ailed Cruz-Collazo, Julia I. Medina   +9 more
openaire   +1 more source

Suppl. Fig. S1 from Characterization of Novel Derivatives of MBQ-167, an Inhibitor of the GTP-binding Proteins Rac/Cdc42

, 2023
<p>Suppl. Fig. S1 shows percentage cell viability</p>
Suranganie Dharmawardhane, Cornelis P. Vlaar, Eliud Hernandez O'Farril, Marcelo G. Kazanietz, Mariana Cooke, Luis D. Borrero-Garcia, Tatiana Matos Gascot, Maria del Mar Maldonado, Ailed Cruz-Collazo, Julia I. Medina   +9 more
openaire   +1 more source

Expression of FGD4 positively correlates with the aggressive phenotype of prostate cancer

BMC Cancer, 2018
Background FGD4 (Frabin) is an F-actin binding protein with GTP/GDP exchange activity specific for CDC42. It is involved in reorganization of the actin cytoskeleton, which requires both actin binding and CDC42 activating function of FGD4.
Alexia Bossan, Richard Ottman, Thomas Andl, Md Faqrul Hasan, Nupam Mahajan, Domenico Coppola, Ratna Chakrabarti   +6 more
doaj   +1 more source