Results 141 to 150 of about 7,812 (168)

Phosphorylation of Cdc6 at serine 74, but not at serine 106, drives translocation of Cdc6 to the cytoplasm

Journal of Cellular Physiology, 2013
AbstractPhosphorylation‐dependent cytoplasmic translocation of human Cdc6 during S phase is sufficient to control its activity after origin firing. Export from the nucleus also serves as a mechanism for preventing re‐replication in mammalian cells. Phosphorylation of the CDK consensus serine residues 54, 74, and 106 has been suggested to be involved in
Hyungshin, Yim, Ji-Woong, Park, Sang Uk, Woo, Seong-Taek, Kim, Linhua, Liu, Chul-Hoon, Lee, Seung Ki, Lee   +6 more
openaire   +2 more sources

Regulation of the localization and stability of Cdc6 in living yeast cells

Biochemical and Biophysical Research Communications, 2003
The Cdc6 protein is an essential regulator for initiation of DNA replication. Following the G1/S transition, Cdc6 is degraded through a ubiquitin-mediated proteolysis pathway. In this study, we tagged Cdc6 with green fluorescent protein (GFP) and used site-specific mutations to study the regulation of Cdc6 localization and degradation in living yeast ...
Kathy Qian Luo, Donald Chang, Judith L Campbell   +2 more
exaly   +4 more sources

The genomic instability of yeast cdc6-1/cdc6-1 mutants involves chromosome structure and recombination

Molecular and General Genetics MGG, 1995
When diploid cells of Saccharomyces cerevisiae homozygous for the temperature-sensitive cell division cycle mutation cdc6-1 are grown at a semipermissive temperature they exhibit elevated genomic instability, as indicated by enhanced mitotic gene conversion, mitotic intergenic recombination, chromosomal loss, chromosomal gain, and chromosomal ...
C V, Bruschi, J N, McMillan, M, Coglievina, M S, Esposito   +3 more
openaire   +2 more sources

LncRNA–CDC6 promotes breast cancer progression and function as ceRNA to target CDC6 by sponging microRNA‐215

Journal of Cellular Physiology, 2018
AbstractRapid proliferation and metastasis of breast cancers resulted in poor prognosis in clinic. Recent studies have proved that long noncoding RNAs (lncRNAs) were involved in tumor progression. In this study, we aimed to determine the roles and mechanisms of lncRNA–cell division cycle 6 (CDC6) in regulating proliferation and metastasis of breast ...
Xiaoli Kong, Yi Duan, Yuting Sang, Yaming Li, Hanwen Zhang, Yiran Liang, Ying Liu, Ning Zhang, Qifeng Yang   +8 more
openaire   +2 more sources

Cdc6 knockdown inhibits human neuroblastoma cell proliferation

Molecular and Cellular Biochemistry, 2008
The cell division controller Cdc6 plays a central role in the initiation of DNA replication. It was found that elevated levels of Cdc6 were present in many of human cancer cells, and the accumulation of Cdc6 is required for cell proliferation. In this study, we have investigated the control of Cdc6 expression and its effect on cell proliferation and ...
Robert Seeger, Lingtao Wu, Ambrose Jong   +2 more
exaly   +3 more sources

Archaeal Orc1/Cdc6 Proteins

2012
The initiation of DNA replication in most archaeal genomes is mediated by proteins related to eukaryotic Orc1 and Cdc6. Archaeal replication origins have been mapped and their interactions with Orc1/Cdc6 proteins have been characterized at the biochemical level. Structural and biophysical studies have revealed the basic rules of sequence recognition by
openaire   +2 more sources

Down to the origin: Cdc6 protein and the competence to replicate

Trends in Cell Biology, 2003
In eukaryotes, DNA replication requires the regulated assembly of pre-replicative complexes (pre-RCs) onto DNA during G1 phase. Pre-RCs render the chromatin competent to replicate, yet it is only at the G1-S phase transition that protein-kinase complexes trigger the transition to DNA replication.
openaire   +2 more sources

Cdc6 and DNA replication: Limited to humble origins

BioEssays, 1996
AbstractThe budding yeast Cdc6 protein is important for regulating DNA replication intiation. Cdc6p acts at replication origins, and cdc6‐1 mutants arrest with unreplicated DNA and show elevated minichromosome loss rates. Overexpression of the related Cdc 18 protein in fission yeast results in DNA rereplication; however, Cdc6p overexpression does not ...
openaire   +2 more sources

Cdc6 expression as a marker of proliferative activity in brain tumors

Oncology Reports, 2001
Antibodies against human proteins that regulate DNA replication such as Cdc6 and Mcm5 became available as a new marker of proliferation. We performed immunohistochemical analysis with MIB-1 and antibody against Cdc6 on 35 brain tumors, including tumors of neuroepithelial tissue, vestibular schwannomas, meningiomas, and pituitary adenomas.
S, Ohta, M, Koide, T, Tokuyama, N, Yokota, S, Nishizawa, H, Namba   +5 more
openaire   +2 more sources

Cdc6 synthesis regulates replication competence in Xenopus oocytes

Nature, 2002
The early division cycles of an embryo rely on the oocyte's ability to replicate DNA. During meiosis, oocytes temporarily lose this ability. After a single round of pre-meiotic S-phase, oocytes enter meiosis and rapidly arrest at prophase of meiosis I (G2).
Elizabeth, Whitmire, Bettina, Khan, Martine, Coué   +2 more
openaire   +2 more sources