Results 151 to 160 of about 4,745 (173)

Genetic dissection of transcriptional regulation by cDNA‐AFLP

The Plant Journal, 2006
SummaryThis study demonstrates that cDNA‐AFLP is a powerful gel‐based genome‐scale transcript profiling technique to generate quantitative gene expression profiles for eQTL mapping. We used cDNA‐AFLP to monitor the relative abundance of 912 transcripts across 50 Arabidopsis thaliana recombinant inbred lines.
Marnik, Vuylsteke, Hilde, Daele, Annelies, Vercauteren, Marc, Zabeau, Martin, Kuiper   +4 more
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Quantitative cDNA-AFLP analysis for genome-wide expression studies

Molecular Genetics and Genomics, 2003
An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microarrays. Unique transcript tags, generated from reverse-transcribed messenger RNA by restriction enzymes, were screened through a series of ...
P, Breyne, R, Dreesen, B, Cannoot, D, Rombaut, K, Vandepoele, S, Rombauts, R, Vanderhaeghen, D, Inzé, M, Zabeau   +8 more
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cDNA AFLP-based techniques for studying transcript profiles in horses

Research in Veterinary Science, 2005
The identification of differentially expressed genes is a fundamental prerequisite for understanding the molecular regulation of most physiological and pathological processes. Among the procedures employed to compare mRNA populations, those that are gel-based appear to hold great promise and are considered excellent tools for studying gene expression ...
CAPPELLI, Katia, Porceddu A., VERINI SUPPLIZI, Andrea, CAPOMACCIO, STEFANO, De Marchis F., FALCINELLI, Mario, GAITI, Alberto, SILVESTRELLI, Maurizio   +7 more
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Transcript Imaging with cDNA-AFLP: A Step-by-Step Protocol

Plant Molecular Biology Reporter, 1998
The method of cDNA-AFLP allows detection of differentially expressed transcripts using PCR. This report provides a detailed and updated protocol for the cDNA-AFLP procedure and an analysis of interactions between its various parameters. We studied the effects of PCR cycle number and template dilution level on the number of transcript derived fragments (
Bachem, C.W.B., Oomen, R.J.F.J., Visser, R.G.F.   +2 more
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AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis

Nature Protocols, 2007
Although DNA microarrays are currently the standard tool for genome-wide expression analysis, their application is limited to organisms for which the complete genome sequence or large collections of known transcript sequences are available. Here, we describe a protocol for cDNA-AFLP, an AFLP-based transcript profiling method that allows genome-wide ...
Marnik, Vuylsteke, Johan D, Peleman, Michiel J T, van Eijk   +2 more
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Linking cDNA-AFLP-based gene expression patterns and ESTs.

Methods in molecular biology (Clifton, N.J.), 2005
Massive amounts of DNA sequence data, generated from expressed sequence tag (EST) and genome sequencing projects, require efficient methods to link sequence databases with temporal and spatial expression profiles. To meet this need, we have developed a powerful computer program (GenEST), which links cDNA sequence data (including EST sequences) with ...
Qin Ling, Prins, J.C.P., Helder, J.
openaire   +2 more sources

Characterization of maize B-chromosome-related transcripts isolated via cDNA-AFLP

Chromosoma, 2014
The maize B-chromosome consists mainly of heterochromatin and is considered to be genetically inert. However, the B-chromosome contains euchromatin that carries control elements that direct its behaviors during cell division, such as nondisjunction during the second pollen mitosis.
Huan-Zhi, Lin, Wei-De, Lin, Chien-Yu, Lin, Shu-Fen, Peng, Ya-Ming, Cheng   +4 more
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Identification of Salt Stress-Induced Transcripts in Potato Leaves by cDNA–AFLP

Molecular Biotechnology, 2005
Potato (Solanum tuberosum L.) is highly sensitive to salt stress, which is one of the most important factors limiting plant cultivation. The investigation of plant response to high salinity was envisaged in this report using cDNA-amplified fragment length polymorphism (AFLP).
HMIDA SAYARI A, COSTA A, LEONE, ANTONIETTA, JAOUA S, GARGOURI BOUZID R.   +4 more
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Construction of a potato transcriptome map based on the cDNA–AFLP technique

Theoretical and Applied Genetics, 2008
The cDNA-AFLP technique can be used to monitor differential gene expression, but also for linkage mapping. Extending previous works, we have now constructed an integrated linkage map of the potato transcriptome based on the said technique that has a length of around 800 cM and contains nearly 700 transcriptome derived fragments (TDFs). At the same time,
Ritter, E., Ruiz de Galarreta, J.I., van Eck, H.J., Sanchez, I.   +3 more
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Identification of sex markers by cDNA-AFLP in Takifugu rubripes

Aquaculture, 2006
Abstract We searched for sex markers in mature male and female Takifugu rubripes gonads and tailfins by cDNA-amplified fragment length polymorphism (AFLP) performed on pooled cDNA samples. Five sex markers (TDF1-5) were identified, cloned, characterized and confirmed on individual samples of cDNA and genomic DNA, showing good agreement with ...
Jian-Zhou Cui, Xue-Yan Shen, Qing-Li Gong, Guan-Pin Yang, Qian-Qun Gu   +4 more
openaire   +1 more source