Results 171 to 180 of about 4,810 (200)

Improvement of the cDNA-AFLP method using fluorescent primers for transcription analysis in bacteria

Journal of Microbiological Methods, 2005
Here the cDNA-fluorescent amplified fragment length polymorphism (cDNA-FAFLP) technique, an improvement of cDNA-AFLP method, was used in order to analyse expression profiling in bacteria. The obtained results were validated by real-time PCR. This is the first report that validates the foreseen transcription pattern by cDNA-FAFLP with the application of
Carlo Viti, Alessio Mengoni
exaly   +4 more sources

Identification of Salt Stress-Induced Transcripts in Potato Leaves by cDNA–AFLP

Molecular Biotechnology, 2005
Potato (Solanum tuberosum L.) is highly sensitive to salt stress, which is one of the most important factors limiting plant cultivation. The investigation of plant response to high salinity was envisaged in this report using cDNA-amplified fragment length polymorphism (AFLP).
HMIDA SAYARI A, COSTA A, LEONE, ANTONIETTA, JAOUA S, GARGOURI BOUZID R.   +4 more
openaire   +3 more sources

Identification of novel p53 target genes by cDNA AFLP in glioblastoma cells

Cancer Letters, 2009
The p53 plays critical role in cellular functions such as cell cycle arrest and apoptosis. We overexpressed wild-type p53 (wt-p53) in U87 glioblastoma cells via recombinant adenovirus Ad-GFP-P53 which encodes p53 and green fluorescent protein. The transcript profiles were investigated using cDNA amplified fragment length polymorphism approach.
Mojtaba, Saffari, Orkideh Saydi, Dinehkabodi, Seyed Hamid, Ghaffari, Mohammad H, Modarressi, Fatemeh, Mansouri, Mansour, Heidari   +5 more
openaire   +2 more sources

Linking cDNA-AFLP-based gene expression patterns and ESTs.

Methods in molecular biology (Clifton, N.J.), 2005
Massive amounts of DNA sequence data, generated from expressed sequence tag (EST) and genome sequencing projects, require efficient methods to link sequence databases with temporal and spatial expression profiles. To meet this need, we have developed a powerful computer program (GenEST), which links cDNA sequence data (including EST sequences) with ...
Qin Ling, Prins, J.C.P., Helder, J.
openaire   +2 more sources

AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis

Nature Protocols, 2007
Although DNA microarrays are currently the standard tool for genome-wide expression analysis, their application is limited to organisms for which the complete genome sequence or large collections of known transcript sequences are available. Here, we describe a protocol for cDNA-AFLP, an AFLP-based transcript profiling method that allows genome-wide ...
Marnik, Vuylsteke, Johan D, Peleman, Michiel J T, van Eijk   +2 more
openaire   +2 more sources

Characterization of maize B-chromosome-related transcripts isolated via cDNA-AFLP

Chromosoma, 2014
The maize B-chromosome consists mainly of heterochromatin and is considered to be genetically inert. However, the B-chromosome contains euchromatin that carries control elements that direct its behaviors during cell division, such as nondisjunction during the second pollen mitosis.
Huan-Zhi, Lin, Wei-De, Lin, Chien-Yu, Lin, Shu-Fen, Peng, Ya-Ming, Cheng   +4 more
openaire   +2 more sources

Molecular Analysis of Drought Tolerance in Tea by cDNA-AFLP Based Transcript Profiling

Molecular Biotechnology, 2012
A cDNA-AFLP approach was used to identify transcript and/or genes specifically expressed in response to drought in tea. Drought was artificially induced and whole genome transcript profiling was done at three different stages-6 days before wilting, 3 days before wilting and at wilting stage of both tolerant and susceptible cultivars, and genetic ...
Sushmita, Gupta, Raju, Bharalee, Priyadarshini, Bhorali, Sourabh K, Das, Prasenjit, Bhagawati, Tirthankar, Bandyopadhyay, Bornali, Gohain, Niraj, Agarwal, Parveen, Ahmed, Sangeeta, Borchetia, M C, Kalita, A K, Handique, Sudripta, Das   +12 more
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Identification of candidate genes for fenvalerate resistance in Helicoverpa armigera using cDNA‐AFLP

Insect Molecular Biology, 2008
Abstract A cDNA‐amplified fragment length polymorphisms approach was undertaken to screen for candidate genes associated with fenvalerate resistance in the AN02 strain of Helicoverpa armigera.
Wee, C., Lee, S., Robin, C., Heckel, D.
openaire   +3 more sources

Expression analysis of stem prickle formation in blackberry using cDNA-AFLP

Acta Horticulturae, 2016
To understand the molecular mechanism of prickle formation in blackberry, differential expression profile of cDNA between 'Boysenberry' blackberry and its thin-thorn mutant were analysed by using cDNA-AFLP. In total, 256 primer pairs were used for the cDNA-AFLP amplification to produce 45 differentially expressed fragments. The putative function of the
C.H. Zhang, W.L. Wu, S.Y. Hu, L.F. Lv, W.L. Li   +4 more
openaire   +1 more source

Technical Advances: Genome-Wide cDNA-AFLP ^® Analysis of the Arabidopsis Transcriptome

OMICS: A Journal of Integrative Biology, 2003
cDNA-AFLP ® , a technology historically used to identify small numbers of differentially expressed genes, was adapted as a genome-wide transcript profiling method. mRNA levels were assayed in a diverse range of tissues from Arabidopsis thaliana
Wayne, Volkmuth, Stefan, Turk, Amy, Shapiro, Yiwen, Fang, Ed, Kiegle, Mark, van Haaren, Jonathan, Donson   +6 more
openaire   +2 more sources