Results 161 to 170 of about 4,810 (200)

Genetic dissection of transcriptional regulation by cDNA-AFLP

Plant Journal, 2006
SummaryThis study demonstrates that cDNA‐AFLP is a powerful gel‐based genome‐scale transcript profiling technique to generate quantitative gene expression profiles for eQTL mapping. We used cDNA‐AFLP to monitor the relative abundance of 912 transcripts across 50 Arabidopsis thaliana recombinant inbred lines.
Martin Tr Kuiper, Hilde Van den Daele
exaly   +3 more sources

Quantitative comparison of cDNA-AFLP, microarrays, and genechip expression data in Saccharomyces cerevisiae

Genomics, 2003
cDNA-AFLP is a genome-wide expression analysis technology that does not require any prior knowledge of gene sequences. This PCR-based technique combines a high sensitivity with a high specificity, allowing detection of rarely expressed genes and distinguishing between homologous genes.
Elzo de Wit
exaly   +5 more sources

cDNA-AFLP-based genetical genomics in cotton fibers [PDF]

Theoretical and Applied Genetics, 2011
Genetical genomics, or genetic analysis applied to gene expression data, has not been widely used in plants. We used quantitative cDNA-AFLP to monitor the variation in the expression level of cotton fiber transcripts among a population of inter-specific Gossypium hirsutum × G. barbadense recombinant inbred lines (RILs).
Claverie, Michel, Souquet, Marlène, Jean, Janine, Forestier-Chiron, Nelly, Lepitre, Vincent, Pré, Martial, Jacobs, John, Llewellyn, Danny, Lacape, Jean-Marc   +8 more
openaire   +3 more sources

cDNA AFLP-based techniques for studying transcript profiles in horses

Research in Veterinary Science, 2005
The identification of differentially expressed genes is a fundamental prerequisite for understanding the molecular regulation of most physiological and pathological processes. Among the procedures employed to compare mRNA populations, those that are gel-based appear to hold great promise and are considered excellent tools for studying gene expression ...
Katia Cappelli, Stefano Capomaccio
exaly   +6 more sources

Transcript Imaging with cDNA-AFLP: A Step-by-Step Protocol

Plant Molecular Biology Reporter, 1998
The method of cDNA-AFLP allows detection of differentially expressed transcripts using PCR. This report provides a detailed and updated protocol for the cDNA-AFLP procedure and an analysis of interactions between its various parameters. We studied the effects of PCR cycle number and template dilution level on the number of transcript derived fragments (
Bachem, C.W.B., Oomen, R.J.F.J., Visser, R.G.F.   +2 more
openaire   +2 more sources

Construction of a potato transcriptome map based on the cDNA–AFLP technique

Theoretical and Applied Genetics, 2008
The cDNA-AFLP technique can be used to monitor differential gene expression, but also for linkage mapping. Extending previous works, we have now constructed an integrated linkage map of the potato transcriptome based on the said technique that has a length of around 800 cM and contains nearly 700 transcriptome derived fragments (TDFs). At the same time,
Ritter, E., Ruiz de Galarreta, J.I., van Eck, H.J., Sanchez, I.   +3 more
openaire   +2 more sources

A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans

Fungal Genetics and Biology, 2006
Expression profiling using cDNA-AFLP is commonly used to display the transcriptome of a specific tissue or developmental stage. Here, cDNA-AFLP was used to identify transcripts in a segregating F1 population of Phytophthora infestans, the oomycete pathogen that causes late blight.
Lars G Kamphuis, Francine Govers
exaly   +4 more sources

Identification of sex markers by cDNA-AFLP in Takifugu rubripes

Aquaculture, 2006
Abstract We searched for sex markers in mature male and female Takifugu rubripes gonads and tailfins by cDNA-amplified fragment length polymorphism (AFLP) performed on pooled cDNA samples. Five sex markers (TDF1-5) were identified, cloned, characterized and confirmed on individual samples of cDNA and genomic DNA, showing good agreement with ...
Jian-Zhou Cui, Xue-Yan Shen, Qing-Li Gong, Guan-Pin Yang, Qian-Qun Gu   +4 more
exaly   +2 more sources

Quantitative cDNA-AFLP analysis for genome-wide expression studies

Molecular Genetics and Genomics, 2003
An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microarrays. Unique transcript tags, generated from reverse-transcribed messenger RNA by restriction enzymes, were screened through a series of ...
P, Breyne, R, Dreesen, B, Cannoot, D, Rombaut, K, Vandepoele, S, Rombauts, R, Vanderhaeghen, D, Inzé, M, Zabeau   +8 more
openaire   +2 more sources

A cDNA-AFLP approach to study ochratoxin A production in Aspergillus carbonarius

International Journal of Food Microbiology, 2008
Aspergillus carbonarius is responsible for the majority of mycotoxin contaminations in grapes and its derivatives. Most of A. carbonarius strains are ochratoxin A (OTA) producers, even though at very different levels. This broad variability was used to identify genes whose expression is linked with the ability of producing OTA. A cDNA-AFLP differential
BOTTON, ALESSANDRO, FERRIGO, DAVIDE, SCOPEL, CRISTINA, CAUSIN, ROBERTO, BONGHI, CLAUDIO, RAMINA, ANGELO   +5 more
openaire   +2 more sources