Results 151 to 160 of about 125,031 (193)

Multi-omics characterization of aflatoxigenic Aspergillus from grains and rhizosphere of maize across agroecological zones of Cameroon. [PDF]

open access: yesSci Rep
Mvogo Nyebe RA   +10 more
europepmc   +1 more source

Bioinformatics in crop research: using genomic data for crop improvement. [PDF]

open access: yesFront Plant Sci
Iqbal MS   +11 more
europepmc   +1 more source

Genetic dissection of transcriptional regulation by cDNA-AFLP

open access: yesPlant Journal, 2006
SummaryThis study demonstrates that cDNA‐AFLP is a powerful gel‐based genome‐scale transcript profiling technique to generate quantitative gene expression profiles for eQTL mapping. We used cDNA‐AFLP to monitor the relative abundance of 912 transcripts across 50 Arabidopsis thaliana recombinant inbred lines.
Marnik Vuylsteke   +2 more
exaly   +4 more sources

cDNA AFLP-based techniques for studying transcript profiles in horses

open access: yesResearch in Veterinary Science, 2005
The identification of differentially expressed genes is a fundamental prerequisite for understanding the molecular regulation of most physiological and pathological processes. Among the procedures employed to compare mRNA populations, those that are gel-based appear to hold great promise and are considered excellent tools for studying gene expression ...
Stefano Capomaccio   +2 more
exaly   +7 more sources

Transcript Imaging with cDNA-AFLP: A Step-by-Step Protocol

Plant Molecular Biology Reporter, 1998
The method of cDNA-AFLP allows detection of differentially expressed transcripts using PCR. This report provides a detailed and updated protocol for the cDNA-AFLP procedure and an analysis of interactions between its various parameters. We studied the effects of PCR cycle number and template dilution level on the number of transcript derived fragments (
Richard G F Visser   +2 more
exaly   +3 more sources

Construction of a potato transcriptome map based on the cDNA–AFLP technique

Theoretical and Applied Genetics, 2008
The cDNA-AFLP technique can be used to monitor differential gene expression, but also for linkage mapping. Extending previous works, we have now constructed an integrated linkage map of the potato transcriptome based on the said technique that has a length of around 800 cM and contains nearly 700 transcriptome derived fragments (TDFs). At the same time,
Ritter, E.   +3 more
openaire   +2 more sources

Quantitative cDNA-AFLP analysis for genome-wide expression studies

Molecular Genetics and Genomics, 2003
An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microarrays. Unique transcript tags, generated from reverse-transcribed messenger RNA by restriction enzymes, were screened through a series of ...
P, Breyne   +8 more
openaire   +2 more sources

A cDNA-AFLP approach to study ochratoxin A production in Aspergillus carbonarius

International Journal of Food Microbiology, 2008
Aspergillus carbonarius is responsible for the majority of mycotoxin contaminations in grapes and its derivatives. Most of A. carbonarius strains are ochratoxin A (OTA) producers, even though at very different levels. This broad variability was used to identify genes whose expression is linked with the ability of producing OTA. A cDNA-AFLP differential
BOTTON, ALESSANDRO   +5 more
openaire   +2 more sources

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