Results 11 to 20 of about 125,031 (193)
Components of variance in transcriptomics based on electrophoretic separation of cDNA fragments (cDNA-AFLP) [PDF]
Electrophoresis, 2009 AbstractThe sources of variance and errors in transcriptomics based on the electrophoretic separation of amplified cDNA fragments were investigated using cDNA‐amplified fragment length polymorphism (AFLP). Transcriptome profiles of the plant‐pathogenic fungus Verticillium longisporum were generated by a standard cDNA‐AFLP protocol followed by ...
Petr Karlovsky
exaly +7 more sources
Optimization of cDNA-AFLP experiments using genomic sequence data [PDF]
Bioinformatics, 2005 cDNA amplified fragment length polymorphism (cDNA-AFLP) is one of the few genome-wide level expression profiling methods capable of finding genes that have not yet been cloned or even predicted from sequence but have interesting expression patterns under the studied conditions.
Kivioja, Teemu +5 more
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Identification of imprinted genes subject to parent-of-origin specific expression in
BMC Plant Biology, 2011 Background Epigenetic regulation of gene dosage by genomic imprinting of some autosomal genes facilitates normal reproductive development in both mammals and flowering plants.
Wennblom Trevor J +14 more
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cDNA-AFLP-based genetical genomics in cotton fibers [PDF]
Theoretical and Applied Genetics, 2011 Genetical genomics, or genetic analysis applied to gene expression data, has not been widely used in plants. We used quantitative cDNA-AFLP to monitor the variation in the expression level of cotton fiber transcripts among a population of inter-specific Gossypium hirsutum × G. barbadense recombinant inbred lines (RILs).
Claverie, Michel +8 more
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AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis
Nature Protocols, 2007 Although DNA microarrays are currently the standard tool for genome-wide expression analysis, their application is limited to organisms for which the complete genome sequence or large collections of known transcript sequences are available. Here, we describe a protocol for cDNA-AFLP, an AFLP-based transcript profiling method that allows genome-wide ...
Marnik, Vuylsteke +2 more
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Linking cDNA-AFLP-based gene expression patterns and ESTs.
Methods in molecular biology (Clifton, N.J.), 2005 Massive amounts of DNA sequence data, generated from expressed sequence tag (EST) and genome sequencing projects, require efficient methods to link sequence databases with temporal and spatial expression profiles. To meet this need, we have developed a powerful computer program (GenEST), which links cDNA sequence data (including EST sequences) with ...
Qin Ling, Prins, J.C.P., Helder, J.
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Identification of Differentially Expressed Genes by cDNA-AFLP in Magnaporthe oryzae [PDF]
Research in Plant Disease, 2019 Analysis of differentially expressed genes has assisted discovery of gene sets involved in particular biological processes. The purpose of this study was to identify genes involved in appressorium formation in the rice blast fungus Magnaporthe oryzae via
Myoung-Hwan Chi, Sook-Young Park
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Identification of ripening-related genes in strawberry fruit by cDNA-AFLP
International Journal of Horticultural Science, 2005 An RNA fingerprinting study of strawberry receptacle and achene tissue was performed to identify candidate genes involved in fruit ripening. Quantitative cDNA-AFLP was used to detect differential gene expression in green, white, pink and red stages of ...
A. Balogh +4 more
doaj +3 more sources
Fungal Genetics and Biology, 2006 Expression profiling using cDNA-AFLP is commonly used to display the transcriptome of a specific tissue or developmental stage. Here, cDNA-AFLP was used to identify transcripts in a segregating F1 population of Phytophthora infestans, the oomycete pathogen that causes late blight.
Jun Guo, J. +3 more
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cDNA-AFLP profiling in the embryo axes during common bean germination
Biologia Plantarum, 2011 cDNA-AFLP fingerprinting was used to identify genes with modulated expression during germination in common bean (Phaseolus vulgaris L.). The analysis was performed on the embryo axes.
B. Teulat-Merah +4 more
doaj +3 more sources