An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences. [PDF]
BACKGROUND: cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge.
Jacob Pollier +4 more
doaj +1 more source
Physiological and transcriptomic evidence for a close coupling between chloroplast ontogeny and cell cycle progression in the pennate diatom Seminavis robusta [PDF]
Despite the growing interest in diatom genomics, detailed time series of gene expression in relation to key cellular processes are still lacking. Here, we investigated the relationships between the cell cycle and chloroplast development in the pennate ...
Chepurnov, Victor A +12 more
core +6 more sources
Role of the Transcription Factor NbTFIISL in Enhancing Bamboo Mosaic Virus Accumulation via Mitochondria Localisation. [PDF]
BaMV infection induces dynamic relocalisation of NbTFIISL and its mutants to mitochondria in Nicotiana benthamiana, as revealed by subcellular colocalisation analysis. ABSTRACT The transcription factor NbTFIISL, a TFIIS‐like protein in Nicotiana benthamiana, plays a critical role in facilitating bamboo mosaic virus (BaMV) infection.
Huang YP +6 more
europepmc +2 more sources
Optimization of cDNA-AFLP experiments using genomic sequence data [PDF]
cDNA amplified fragment length polymorphism (cDNA-AFLP) is one of the few genome-wide level expression profiling methods capable of finding genes that have not yet been cloned or even predicted from sequence but have interesting expression patterns under the studied conditions.
Kivioja, Teemu +5 more
openaire +4 more sources
Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain. Abstract Aspergillus flavus is a ubiquitous saprophyte and opportunistic fungal pathogen with worldwide distribution, which causes diseases commonly in agricultural commodities and seriously threatens food security and human health. In this
Chao Ding +6 more
wiley +1 more source
Summary The rubber tree (Hevea brasiliensis) is grown in tropical regions and is the major source of natural rubber. Using traditional breeding approaches, the latex yield has increased by sixfold in the last century. However, the underlying genetic basis of rubber yield improvement is largely unknown.
Han Cheng +12 more
wiley +1 more source
Abstract Understanding the genetic targets of natural selection is one of the most challenging goals of population genetics. Some of the earliest candidate genes were identified from associations between allozyme allele frequencies and environmental variation.
Alan Le Moan +7 more
wiley +1 more source
Developing resistance to Fusarium wilt in chickpea: From identifying meta-QTLs to molecular breeding. [PDF]
Abstract Fusarium wilt (FW) significantly affects the growth and development of chickpea (Cicer arietinum L.), leading to substantial economic losses. FW resistance is a quantitative trait that is controlled by multiple genomic regions. In this study, a meta‐analysis was conducted on 32 quantitative trait loci (QTLs) associated with FW resistance ...
Soorni J +8 more
europepmc +2 more sources
Summary A gene upregulated in Nicotiana benthamiana after Bamboo mosaic virus (BaMV) infection was revealed as 1‐deoxy‐d‐xylulose‐5‐phosphate reductoisomerase (NbDXR). DXR is the key enzyme in the 2‐C‐methyl‐d‐erythritol‐4‐phosphate (MEP) pathway that catalyzes the conversion of 1‐deoxy‐d‐xylulose 5‐phosphate to 2‐C‐methyl‐d‐erythritol‐4‐phosphate ...
Ying‐Ping Huang +4 more
wiley +1 more source
Profiling and quantifying differential gene transcription provide insights into ganoderic acid biosynthesis in Ganoderma lucidum in response to methyl jasmonate. [PDF]
Ganoderma lucidum is a mushroom with traditional medicinal properties that has been widely used in China and other countries in Eastern Asia. Ganoderic acids (GA) produced by G. lucidum exhibit important pharmacological activities.
Ang Ren +6 more
doaj +1 more source

