Results 1 to 10 of about 284,909 (310)

Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5 [PDF]

BMC Research Notes, 2019
Objective Bacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium Pseudomonas syringae pv. actinidiae (Psa).
Karthikeyan Dharmaraj, Wei Cui, Erik H. A. Rikkerink, Matthew D. Templeton   +3 more
doaj   +2 more sources

In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. [PDF]

PLoS ONE, 2012
The cDNA expression libraries that produce correct proteins are essential in facilitating the identification of protein-protein interactions. The 5'-untranslated regions (UTRs) that are present in the majority of mammalian and non-mammalian genes are ...
Sangkyou Lee, Ilkyun Lee, Yoonsuh Jung, David McConkey, Bogdan Czerniak   +4 more
doaj   +2 more sources

Enhancing biological signals and detection rates in single-cell RNA-seq experiments with cDNA library equalization. [PDF]

Nucleic Acids Res, 2022
Bacher R, Chu LF, Argus C, Bolin JM, Knight P, Thomson JA, Stewart R, Kendziorski C.   +7 more
europepmc   +3 more sources

cDNA Library Screening Using the SOS Recruitment System [PDF]

BioTechniques, 2001
The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions.
Wendong Huang, Sung-ling Wang, Guillermina Lozano, Benoit de Crombrugghe   +3 more
doaj   +2 more sources

cDNA library construction from a small amount of RNA: adaptor-ligation approach for two-round cRNA amplification using T7 and SP6 RNA polymerases

BioTechniques, 2005
In this study, we developed a method that allows cDNA library construction from a small amount of RNA without causing serious size bias in the resulting cDNA population.
Reiko Ohara, Reiko F. Kikuno, Hiroshi Kitamura, Osamu Ohara   +3 more
doaj   +2 more sources

A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library. [PDF]

Virol J, 2015
Waugh C, Cromer D, Grimm A, Chopra A, Mallal S, Davenport M, Mak J.   +6 more
europepmc   +3 more sources

Primary analysis of the expressed sequence tags in a pentastomid nymph cDNA library. [PDF]

PLoS One, 2013
Zhang J, Shen Y, Yuan Z, Yin J, Zang W, Xu Y, Lu W, Wang Y, Wang Y, Cao J.   +9 more
europepmc   +3 more sources

Construction and identification of yeast two-hybrid cDNA library of Haemonchus contortus

浙江大学学报. 农业与生命科学版, 2022
This experiment aimed to construct a cDNA library of Haemonchus contortus, which could provide the basis for further research on the protein interaction mechanism and screening out the interactive proteins of H. contortus.
ZHANG Hui, HUANG Yan, ZHOU Jingru, WU Fei, TONG Danni, CHEN Xueqiu, YANG Yi, MA Guangxu, DU Aifang   +8 more
doaj   +1 more source

Identification of Multiple Stress Responsive Genes by Sequencing a Normalized cDNA Library from Sea-Land Cotton (Gossypium barbadense L.). [PDF]

PLoS One, 2016
Zhou B, Zhang L, Ullah A, Ullah A, Jin X, Yang X, Zhang X.   +6 more
europepmc   +3 more sources

An improved shotgun antisense method for mutagenesis and gene identification

BioTechniques, 2020
Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in Dictyostelium discoideum. However, the method has two limitations.
Yu Tang, Richard H Gomer
doaj   +1 more source