Human interleukin-1 receptor antagonist is expressed in liver [PDF]
, 1992 Using PCR and Northern blot analysis, an IL-1 receptor antagonist specific transcript was amplified from HepG2- and liver mRNA, cDNA clones coding for IL-1 receptor antagonist were isolated from a liver cDNA library and sequence comparison revealed ...
Estaller, C. +3 more
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Molecular cloning and sequence analysis of the cDNA encoding the human acrosin-trypsin inhibitor (HUSI-II) [PDF]
, 1991 A complete cDNA clone encoding the human acrosin-trypsin inhibitor HUSI-II has been isolated from a cDNA library of human testis and completely sequenced.
Fink, Edwin +2 more
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PCR-based cDNA library construction: general cDNA libraries at the level of a few cells [PDF]
Nucleic Acids Research, 1989 A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers complementary to oligo(dA) and oligo(dG) ends of the ...
T. Vinogradova +2 more
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cDNA-detector: Detection and removal of cDNA contamination in DNA sequencing libraries [PDF]
BMC Bioinformatics, 2021 SummaryExogenous cDNA introduced into an experimental system, either intentionally or accidentally, can appear as added read coverage over that gene in next-generation sequencing libraries derived from this system. If not properly recognized and managed, this cross-contamination with exogenous signal can lead to incorrect interpretation of research ...
Utthara Nayar +7 more
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Создание библиотеки кДНК из меристемы метелки пальчатого проса [PDF]
, 2014 Апробирован протокол получения полноразмерной кДНК на основе набора SuperScript® Full-Length cDNA Library Construction Kit II («Invitrogen» США) и роздана высококачественная библиотека кДНК из меристематической ткани метелки пальчатого проса (Eleusine ...
Блюм, Я.Б. +4 more
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A novel vector for construction of cDNA library [PDF]
Biomeditsinskaya Khimiya, 2010 A new original vector pEM-(dT)40(f+) has been prepared. It can be used for cDNA library construction from polyadenylated mRNA, isolated from various sources. The pGEM-(dT)40f(+) is initially transformed into single stranded and then into a linear form and its (dT)40 tail at 3'-end is used as the vector-primer for synthesis of the first strand cDNA. The
V. I. Fedchenko +2 more
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Tissue-specific Expression of Distinct Spectrin and Ankyrin Transcripts in Erythroid and Nonerythroid Cells [PDF]
, 1985 cDNA probes for three components of the erythroid membrane skeleton, α spectrin, β spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a λgt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)^+
Lazarides, Elias +3 more
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The isolation of differentially expressed cDNA clones from the filarial nematode Brugia pahangi [PDF]
, 1999 A cDNA library constructed from 3 day post-infective L3 of the filarial nematode Brugia pahangi was screened by differential hybridization with cDNA probes prepared from different life-cycle stages.
Devaney, E. +4 more
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cDNA Cloning Demonstrates the Expression of Pregnancy-Specific Glycoprotein Genes, a Subgroup of the Carcinoembryonic Antigen Gene Family, in Fetal Liver [PDF]
, 1989 The pregnancy-specific glycoprotein (PSG) genes constitute a subgroup of the carcinoembryonic antigen (CEA) gene family. Here we report the cloning of four cDNAs coding for different members of the PSG family from a human fetal liver cDNA library.
Thompson, John A. +2 more
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, 2023 As a pioneer biotechnology company in the world, Creative Biogene can provide high quality cDNA libraries construction service to customers worldwide. Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library, subtractive cDNA ...
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