Results 31 to 40 of about 405,232 (337)
Journal of Limnology, 2007 A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN +3 more
doaj +1 more source
Scientific Reports, 2022 The full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants.
Hiroki Ura, Sumihito Togi, Yo Niida
doaj +1 more source
BioTechniques, 2001 Here, we describe the application of a RecA-based cloning technology to generate full-length cDNA libraries enriched for genes that are differentially expressed between tumor and normal tissue samples.
B. Zhumabayeva +4 more
doaj +1 more source
, 2023 Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library, subtractive cDNA library, normalized cDNA library, full length cDNA library, yeast two-hybrid cDNA library and SSH cDNA library.
openaire +2 more sources
Development of a Yeast Trihybrid Screen Using Stable Yeast Strains and Regulated Protein Expression
BioTechniques, 1998 We describe a yeast trihybrid system that facilitates rapid screening of cDNA libraries. Novel yeast vectors were developed that direct integration of cDNA encoding the bait and third protein component into the yeast chromosome.
K.J. Fuller +4 more
doaj +1 more source
Analysis of expressed genes in normal and tumoral mammary gland tissue of the terrier dog [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2018 Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in
Nehir OZDEMIR OZGENTURK +9 more
doaj +1 more source
Protocol for Metatranscriptomic analysis of Intestinal Microbiota [PDF]
, 2012 The objective of this publication is to provide the detailed protocol for metartancriptomisc studies of animal intestinal microbiota. The protocol describes isolation of high quality microbial community RNA from the mammalian intestinal content ...
Alvaro Hernandez +4 more
core +2 more sources
Display of a Maize cDNA library on baculovirus infected insect cells
BMC Biotechnology, 2008 Background Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies for efficient proteomic analysis ...
Jones Ian M +4 more
doaj +1 more source
Assessment of Ion S5 NGS protocol for SARS-CoV-2 genome sequencing
Genetics & Applications, 2021 Monitoring of the lineages SARS-CoV-2 is equally important in a fight against COVID-19 epidemics, as is regular RT - PCR testing. Ion AmpliSeq Library kit plus is a robust and validated protocol for library preparation, but certain optimizations for
Dino Pećar +3 more
doaj +1 more source
cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle [PDF]
, 1995 PCR was used to amplify the coding region of CHO MCT1 cDNA. This was then used to screen a rat skeletal muscle cDNA library which lead to the isolation of a full length cDNA encoding MCT1 from rat.
Halestrap, Andrew P. +2 more
core +1 more source