Results 51 to 60 of about 284,909 (310)
Molecular Oncology, EarlyView.Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad +12 more
wiley +1 more source
Production of
BMC Biotechnology, 2007 Background Many structural biology- and high-throughput laboratories experience the acquisition of multiple cDNAs from different sources as a rather time- and resource-consuming procedure. The techniques presented here solve these problems.
Steinmetz Michel O +3 more
doaj +1 more source
Molecular Oncology, EarlyView.The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola +12 more
wiley +1 more source
3′ RACE Walking along a Large cDNA Employing Tiered Suppression PCR
BioTechniques, 2003 Large genes present particular cloning difficulties, especially when expressed at relatively low levels. We describe a novel method, termed 3′ rapid amplification of cDNA ends (RACE) walking, for the rapid determination of unknown 3′ flanking sequence of
Daniel J. Park +3 more
doaj +1 more source
Scientific Reports, 2021 RNA sequencing enables high-content/high-complexity measurements in small molecule screens. Whereas the costs of DNA sequencing and RNA-seq library preparation have decreased consistently, RNA extraction remains a significant bottleneck to scalability ...
Shreya Ghimire +3 more
doaj +1 more source
PCR-based cDNA library construction: general cDNA libraries at the level of a few cells [PDF]
Nucleic Acids Research, 1989 A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers complementary to oligo(dA) and oligo(dG) ends of the ...
A, Belyavsky +2 more
openaire +3 more sources
Meiotic cDNA libraries reveal gene truncations and mitochondrial proteins important for competitive fitness in Saccharomyces cerevisiae [PDF]
, 2022 Tina L. Sing +9 more
openalex +1 more source
ATF4‐mediated stress response as a therapeutic vulnerability in chordoma
Molecular Oncology, EarlyView.We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone +11 more
wiley +1 more source
Preparation and quality detection on gene chip of head cDNA from Apis cerana cerana
浙江大学学报. 农业与生命科学版, 2009 Through sequencing of the head cDNA library of Chinese honeybee (Apis cerana cerana) and sequence analysis, 8569 high quality EST sequences were obtained.
YANG Yu-xiao +4 more
doaj +1 more source
Molecular Oncology, EarlyView.Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee +8 more
wiley +1 more source