Antimicrobial Peptides from Plants: A cDNA-Library Based Isolation, Purification, Characterization Approach and Elucidating Their Modes of Action. [PDF]
Islam MS+6 more
europepmc +1 more source
A multifunctional hydroxyapatite (HAp) coating integrated with silver‐gallium liquid metal nanoparticles (HAp‐Ag‐GaNPs) exhibits dual antibacterial and osteogenic properties. It effectively inhibits Gram‐positive and Gram‐negative bacteria, including resistant strains, while enhancing bone regeneration.
Ngoc Huu Nguyen+17 more
wiley +1 more source
Screening a Spliced Leader-Based Symbiodinium microadriaticum cDNA Library Using the Yeast-Two Hybrid System Reveals a Hemerythrin-Like Protein as a Putative SmicRACK1 Ligand. [PDF]
Islas-Flores T+2 more
europepmc +1 more source
Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification [PDF]
Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is still required for cDNA library preparation from single ...
Baugh+21 more
core +2 more sources
An ‘equalized cDNA library’ by the reassociation of short double-stranded cDNAs
The total number of genes in higher organisms is estimated to be under one hundred thousand. However, constructing a cDNA library containing a full set of genes expressed throughout the life time of an organism, without redundancy, is a major challenge for modern biology.
openaire +3 more sources
Highly sprouting organoid‐like neurovascular spheroids (NVUs) are developed, featuring cell‐loaded poly‐3‐hydroxybutyrate 4‐hydroxybutyrate(P34HB) porous microsphere cores embedded within Gelatin Methacryloyl. NVUs formed complex vascular plexuses and secreted extracellular matrix in vitro, simulating autologous nerves and blood interaction.
Junjin Jie+5 more
wiley +1 more source
Isolation And Characterization Of Disease And Stress Related Genes From Muskmelon (Cucumis Melo L.) [PDF]
Soil salinity and attack by pathogen a re the major abiotic stresses in plant agriculture worldwide. Past efforts to improve plant tolerance to osmotic stress and pathogen attack through breeding and genetic engineering have shown limited success ...
Lee, Weng Wah
core
Spatially Controlled 3‐D Multiplexed Aptamer Patterning in Hydrogels
A hydrogel platform based on norbornene‐functionalized polyvinyl alcohol enables high‐resolution, 3‐D multiplexed patterning of DNA aptamers via two‐photon polymerization. Two distinct aptamers are covalently immobilized with single micron‐scale precision across the x, y, and z dimensions.
Kevin Roost+9 more
wiley +1 more source
Emerging Therapeutic Strategies for Hearing Loss
Challenges still exist in treating hearing loss in cases of severe damage to hair cells or spiral ganglion neurons. Here, a schematic diagram of cochlear sensory hair cells and auditory nerves is presented. It is found that in normal Corti organs, hair cells have upright stereocilia at the top and auditory neurons at the base. Ototoxic drugs, noise, or
Shanying Han+9 more
wiley +1 more source
CDNA library from the Latex of Hevea brasiliensis [PDF]
Latex from Hevea brasiliensis contains 30-50% (w/w) of natural rubber (cis-1,4-polyisoprene), the important rawmaterial for many rubber industries. We have constructed a cDNA library from the latex of H. brasiliensis to investigate theexpressed genes and
Amornrat Phongdara+2 more
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