Results 81 to 90 of about 284,909 (310)

SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones

BMC Genomics, 2004
Background cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high percentage of 5'-truncated clones.
Poustka Annemarie, Schupp Ingo, Wellenreuther Ruth, Wiemann Stefan   +3 more
doaj   +1 more source

Molecular Diversity of Peptide Toxins in the Venom of Spider Heteropoda pingtungensis as Revealed by cDNA Library and Transcriptome Sequencing Analysis. [PDF]

Toxins (Basel), 2022
Liao Q, Kong X, Luo G, Wu X, Li Y, Liu Q, Tang C, Liu Z.   +7 more
europepmc   +1 more source

Decellularized Extracellular Matrix Scaffolds to Engineer the Dormant Landscape of Microscopic Colorectal Cancer Liver Metastasis

Advanced Healthcare Materials, EarlyView.
Decellularized liver extracellular matrix scaffolds provide a platform to study dormant liver‐metastatic colorectal cancer. They induce reversible dormancy, in combination with nutrient depletion and low dose chemotherapy, through cell cycle arrest and chemotherapy resistance.
Sabrina N. VandenHeuvel, Lucia L. Nash, Abigail J. Clevenger, Claudia A. Collier, Oscar R. Benavides, Sanjana Roy, Brinlee Goggans, Aelita Salikhova, Anvitha Tharakesh, Svasti Haricharan, Amber N. Stratman, Scott Kopetz, Alex J. Walsh, Shreya A. Raghavan   +13 more
wiley   +1 more source

Excess primer degradation by Exo I improves the preparation of 3′ cDNA ligation-based sequencing libraries

BioTechniques, 2019
RNA sequencing library construction using single-stranded ligation of a DNA adapter to 3′ ends of cDNAs often produces primer–adapter byproducts, which compete with cDNA–adapter ligation products during library amplification and, therefore, reduces the ...
Christel Hougård Enroth, Annaleigh Ohrt Fehler, Line Dahl Poulsen, Jeppe Vinther   +3 more
doaj   +1 more source

A simple and efficient method for constructing an adenoviral cDNA expression library [PDF]

, 2003
Kazuteru Hatanaka, Shumpei Ohnami, Kimiko Yoshida, Yoshiaki Miura, Kazuhiko Aoyagi, Hiroki Sasaki, Masahiro Asaka, Masaaki Terada, Teruhiko Yoshida, Kazunori Aoki   +9 more
openalex   +1 more source

A Human Kidney Tubuloid Model of Repeated Cisplatin‐Induced Cellular Senescence and Fibrosis for Drug Screening

Advanced Healthcare Materials, EarlyView.
Replicating aging and senescence‐related pathophysiological responses in kidney organoids remains a significant challenge. Human adult renal tubular organoid, tubuloids, are successfully developed recapitulating cellular senescence that is the central pathophysiological mechanism of chronic kidney disease (CKD).
Yuki Nakao, Makiko Mori, Yuta Sekiguchi, Iori Morita, Ryota Shindoh, Shintaro Mandai, Tamami Fujiki, Hiroaki Kikuchi, Fumiaki Ando, Koichiro Susa, Takayasu Mori, Ayumi Suzuki, Yuji Nashimoto, Hirokazu Kaji, Yuma Waseda, Soichiro Yoshida, Yasuhisa Fujii, Eisei Sohara, Shinichi Uchida, Kensuke Miyake, Yutaro Mori   +20 more
wiley   +1 more source

Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

Memorias do Instituto Oswaldo Cruz, 2007
The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any
Luciana Girotto Gentil, Fernanda Lasakosvitsch, José Franco da Silveira, Márcia Regina Machado dos Santos, Clara Lúcia Barbiéri   +4 more
doaj   +1 more source

Improved Functional Expression of Cytochrome P450s in Saccharomyces cerevisiae Through Screening a cDNA Library From Arabidopsis thaliana. [PDF]

Front Bioeng Biotechnol, 2021
Jiang L, Dong C, Liu T, Shi Y, Wang H, Tao Z, Liang Y, Lian J.   +7 more
europepmc   +1 more source

Geometrically Tunable Scaffold‐Free Muscle Bioconstructs for Treating Volumetric Muscle Loss

Advanced Healthcare Materials, EarlyView.
Volumetric muscle loss is associated with traumatic muscle resulting in permanent functional impairment. Mold‐based, scaffold‐free, high‐density muscle tissue bioconstructs are developed in customizable geometric shapes and sizes. The transplanted rectangular solid‐shaped muscle bioconstructs improved muscle force recovery and tissue regeneration in ...
Bugra Ayan, Gaoxian Chen, Ishita Jain, Sha Chen, Gladys Chiang, Caroline Hu, Renato Reyes, Beu P. Oropeza, Ngan F. Huang   +8 more
wiley   +1 more source

A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol. [PDF]

Nucleic Acids Res, 2021
Anastasakis DG, Jacob A, Konstantinidou P, Meguro K, Claypool D, Cekan P, Haase AD, Hafner M.   +7 more
europepmc   +1 more source