Cdt1 is differentially targeted for degradation by anticancer chemotherapeutic drugs. [PDF]
PLoS ONE, 2012 BACKGROUND: Maintenance of genome integrity is crucial for the propagation of the genetic information. Cdt1 is a major component of the pre-replicative complex, which controls once per cell cycle DNA replication. Upon DNA damage, Cdt1 is rapidly targeted
Athanasia Stathopoulou +7 more
doaj +4 more sources
The prognostic significance of Cdc6 and Cdt1 in breast cancer [PDF]
Scientific Reports, 2017 DNA replication is a critical step in cell proliferation. Overexpression of MCM2-7 genes correlated with poor prognosis in breast cancer patients. However, the roles of Cdc6 and Cdt1, which work with MCMs to regulate DNA replication, in breast cancers ...
Ravikiran Mahadevappa +8 more
doaj +5 more sources
USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication [PDF]
Molecular Oncology, 2016 DNA replication control is a key process in maintaining genomic integrity. Monitoring DNA replication initiation is particularly important as it needs to be coordinated with other cellular events and should occur only once per cell cycle. Crucial players
Santiago Hernández-Pérez +7 more
doaj +5 more sources
Temporal control of human DNA replication licensing by CDK4/6-RB signalling and chemical genetics [PDF]
Nature CommunicationsCyclin-dependent kinases (CDKs) coordinate DNA replication and cell division, and play key roles in tissue homeostasis, genome stability and cancer development.
Anastasia Sosenko Piscitello +11 more
doaj +2 more sources
MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis [PDF]
Nature CommunicationsThe eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre ...
Sarah V. Faull +11 more
doaj +2 more sources
Cdt1 stabilizes an open MCM ring for helicase loading [PDF]
Nature Communications, 2017 The loading and activation of the Mcm2-7 replicative helicase couples cell cycle progression to DNA replication. Here the authors use X-ray crystallography and single-particle electron microscopy to demonstrate how Ctd1 functions to promote MCM loading ...
Jordi Frigola +9 more
doaj +6 more sources
Stabilisation of half MCM ring by Cdt1 during DNA insertion [PDF]
Nature Communications, 2021 During pre-Replication Complex, eukaryotic cells load two MCMs into a head-to-head Double Hexamer around duplex DNA (DH). Here the authors preRC assembly assay with purified proteins to reveal insights into S. cerevisiae’s first steps that lead to the DH
Marina Guerrero-Puigdevall +2 more
doaj +4 more sources
Unidirectional MCM translocation away from ORC drives origin licensing [PDF]
Nature CommunicationsThe MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. ATP binding supports formation of the ORC-Cdc6-Cdt1-MCM (OCCM) helicase-recruitment complex where ORC ...
Agata Butryn +2 more
doaj +2 more sources
A novel homozygous intronic variant in CDT1 that alters splicing causes Meier–Gorlin syndrome, and a review of published mutations and growth hormone treatments [PDF]
Orphanet Journal of Rare DiseasesBackground Meier–Gorlin syndrome (MGORS) is a rare autosomal inherited form of primordial dwarfism. Pathogenic variants in 13 genes involved in DNA replication initiation have been identified in this disease, but homozygous intronic variants have never ...
Qing Li +5 more
doaj +2 more sources
Identification of CDT1 as a prognostic marker in human lung adenocarcinoma using bioinformatics approaches [PDF]
PeerJ, 2023 Background Lung cancer has the highest cancer-related mortality worldwide. Lung adenocarcinoma (LUAD) is the most common histological subtype of non-small cell lung cancer (NSCLC).
Jing Jiang +12 more
doaj +2 more sources