Results 21 to 30 of about 5,045 (159)
Frontiers in Pharmacology, 2022 DNA replication initiation requires the loading of MCM2-7 complexes at the origins of replication during G1. Replication licensing renders chromatin competent for DNA replication and its tight regulation is essential to prevent aberrant DNA replication ...
Nikolaos Karantzelis +10 more
doaj +1 more source
Mitotic UV irradiation induces a DNA replication-licensing defect that potentiates G1 arrest response. [PDF]
PLoS ONE, 2015 Cdt1 begins to accumulate in M phase and has a key role in establishing replication licensing at the end of mitosis or in early G1 phase. Treatments that damage the DNA of cells, such as UV irradiation, induce Cdt1 degradation through PCNA-dependent CRL4-
Masayuki Morino +6 more
doaj +1 more source
The SCFSkp2 Ubiquitin Ligase Complex Interacts with the Human Replication Licensing Factor Cdt1 and Regulates Cdt1 Degradation [PDF]
Journal of Biological Chemistry, 2003 DNA replication initiation is tightly controlled so that each origin only fires once per cell cycle. Cell cycle-dependent Cdt1 degradation plays an essential role in DNA replication control, as overexpression of Cdt1 leads to re-replication. In this study, we investigated the mechanisms of Cdt1 degradation in mammalian cells.
Xianghong, Li +4 more
openaire +2 more sources
Regulation of DNA Replication Licensing and Re-Replication by Cdt1 [PDF]
International Journal of Molecular Sciences, 2021 In eukaryotic cells, DNA replication licensing is precisely regulated to ensure that the initiation of genomic DNA replication in S phase occurs once and only once for each mitotic cell division. A key regulatory mechanism by which DNA re-replication is suppressed is the S phase-dependent proteolysis of Cdt1, an essential replication protein for ...
openaire +3 more sources
Mouse Geminin Inhibits Not Only Cdt1-MCM6 Interactions but Also a Novel Intrinsic Cdt1 DNA Binding Activity [PDF]
Journal of Biological Chemistry, 2002 DNA replication is controlled by the stepwise assembly of a pre-replicative complex and the replication apparatus. Cdt1 is a novel component of the pre-replicative complex and plays a role in loading the minichromosome maintenance (MCM) 2-7 complex onto chromatin.
Ken-ichiro, Yanagi +3 more
openaire +2 more sources
SIRT3 promotion reduces resistance to cisplatin in lung cancer by modulating the FOXO3/CDT1 axis
Cancer Medicine, 2021 Background Cisplatin is an extensively used chemotherapy agent for lung cancer, but its drug resistance serves as a huge obstacle for chemotherapy failure of lung cancer patients.
Yang Cao +4 more
doaj +1 more source
A helicase-tethered ORC flip enables bidirectional helicase loading
eLife, 2021 Replication origins are licensed by loading two Mcm2-7 helicases around DNA in a head-to-head conformation poised to initiate bidirectional replication. This process requires origin–recognition complex (ORC), Cdc6, and Cdt1.
Shalini Gupta +3 more
doaj +1 more source
Functional domains of the Xenopus replication licensing factor Cdt1 [PDF]
Nucleic Acids Research, 2005 During late mitosis and early G1, replication origins are licensed for subsequent replication by loading heterohexamers of the mini-chromosome maintenance proteins (Mcm2-7). To prevent re-replication of DNA, the licensing system is down-regulated at other cell cycle stages.
Ferenbach, Andrew +3 more
openaire +3 more sources
Checkpoint kinase ATR phosphorylates Cdt2, a substrate receptor of CRL4 ubiquitin ligase, and promotes the degradation of Cdt1 following UV irradiation. [PDF]
PLoS ONE, 2012 The DNA replication-licensing factor Cdt1 is present during the G1 phase of the cell cycle. When cells initiate S phase or are UV-irradiated, Cdt1 is recruited to chromatin-bound PCNA and ubiquitinated by CRL4(Cdt2) for degradation.
Hiroki Sakaguchi +7 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2021 Obstructive sleep apnea-hypopnea (OSAH) is correlated with an increased incidence of lung cancer. In our study, we explored the functional roles of microRNAs (miRNAs) in lung cancer patients that were complicated with OSAH involving the deubiquitination ...
Weihao Li +6 more
doaj +1 more source