Results 21 to 30 of about 6,157 (185)

Inhibition of Eukaryotic DNA Replication by Geminin Binding to Cdt1

Science, 2000
In all eukaryotic organisms, inappropriate firing of replication origins during the G 2 phase of the cell cycle is suppressed by cyclin-dependent kinases. Multicellular eukaryotes contain a second putative inhibitor of re-replication called geminin.
J A, Wohlschlegel, B T, Dwyer, S K, Dhar, C, Cvetic, J C, Walter, A, Dutta   +5 more
openaire   +3 more sources

HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1 [PDF]

Genes & Development, 2008
HBO1 histone acetylase is important for DNA replication licensing. In human cells, HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin.
Miotto, Benoit, Struhl, Kevin
openaire   +4 more sources

Stabilisation of half MCM ring by Cdt1 during DNA insertion [PDF]

Nature Communications, 2021
During pre-Replication Complex, eukaryotic cells load two MCMs into a head-to-head Double Hexamer around duplex DNA (DH). Here the authors preRC assembly assay with purified proteins to reveal insights into S. cerevisiae’s first steps that lead to the DH
Marina Guerrero-Puigdevall, Narcis Fernandez-Fuentes, Jordi Frigola   +2 more
doaj   +4 more sources

CDT1 acts with the replisome to remodel replication forks. [PDF]

Sci Adv
Replication forks encounter problems during genome duplication that trigger replication stress. Fork reversal is a key stress tolerance pathway that helps mitigate replication challenges to facilitate DNA synthesis. Here, we report the function of origin
Hathaway C, Le T, Hatoyama Y, Kanemaki M, Dungrawala H.   +4 more
europepmc   +2 more sources

Novel Phosphorylation Sites on Cdt1 Regulate Its Activity [PDF]

, 2016
Origin licensing is a process responsible for preparing the genome for replication during the cell cycle. The overexpression of chromatin licensing and DNA replication factor 1 (Cdt1), an origin licensing protein, is toxic to cells because it induces re-replication of DNA.
Cole, Yasemin
openaire   +2 more sources

Unidirectional MCM translocation away from ORC drives origin licensing [PDF]

Nature Communications
The MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. ATP binding supports formation of the ORC-Cdc6-Cdt1-MCM (OCCM) helicase-recruitment complex where ORC ...
Agata Butryn, Julia F. Greiwe, Alessandro Costa   +2 more
doaj   +2 more sources

Small Molecule Inhibitor Targeting CDT1/Geminin Protein Complex Promotes DNA Damage and Cell Death in Cancer Cells

Frontiers in Pharmacology, 2022
DNA replication initiation requires the loading of MCM2-7 complexes at the origins of replication during G1. Replication licensing renders chromatin competent for DNA replication and its tight regulation is essential to prevent aberrant DNA replication ...
Nikolaos Karantzelis, Michalis Petropoulos, Valeria De Marco, David A. Egan, Alexander Fish, Evangelos Christodoulou, David W. Will, Joe D. Lewis, Anastassis Perrakis, Zoi Lygerou, Stavros Taraviras   +10 more
doaj   +1 more source

Mitotic UV irradiation induces a DNA replication-licensing defect that potentiates G1 arrest response. [PDF]

PLoS ONE, 2015
Cdt1 begins to accumulate in M phase and has a key role in establishing replication licensing at the end of mitosis or in early G1 phase. Treatments that damage the DNA of cells, such as UV irradiation, induce Cdt1 degradation through PCNA-dependent CRL4-
Masayuki Morino, Kohei Nukina, Hiroki Sakaguchi, Takeshi Maeda, Michiyo Takahara, Yasushi Shiomi, Hideo Nishitani   +6 more
doaj   +1 more source

The SCFSkp2 Ubiquitin Ligase Complex Interacts with the Human Replication Licensing Factor Cdt1 and Regulates Cdt1 Degradation [PDF]

Journal of Biological Chemistry, 2003
DNA replication initiation is tightly controlled so that each origin only fires once per cell cycle. Cell cycle-dependent Cdt1 degradation plays an essential role in DNA replication control, as overexpression of Cdt1 leads to re-replication. In this study, we investigated the mechanisms of Cdt1 degradation in mammalian cells.
Xianghong, Li, Qiping, Zhao, Rong, Liao, Peiqing, Sun, Xiaohua, Wu   +4 more
openaire   +2 more sources

Mouse Geminin Inhibits Not Only Cdt1-MCM6 Interactions but Also a Novel Intrinsic Cdt1 DNA Binding Activity [PDF]

Journal of Biological Chemistry, 2002
DNA replication is controlled by the stepwise assembly of a pre-replicative complex and the replication apparatus. Cdt1 is a novel component of the pre-replicative complex and plays a role in loading the minichromosome maintenance (MCM) 2-7 complex onto chromatin.
Ken-ichiro, Yanagi, Takeshi, Mizuno, Zhiying, You, Fumio, Hanaoka   +3 more
openaire   +2 more sources