Results 21 to 30 of about 5,745 (188)

Inhibition of Eukaryotic DNA Replication by Geminin Binding to Cdt1

Science, 2000
In all eukaryotic organisms, inappropriate firing of replication origins during the G 2 phase of the cell cycle is suppressed by cyclin-dependent kinases. Multicellular eukaryotes contain a second putative inhibitor of re-replication called geminin.
J A, Wohlschlegel, B T, Dwyer, S K, Dhar, C, Cvetic, J C, Walter, A, Dutta   +5 more
openaire   +3 more sources

HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1 [PDF]

Genes & Development, 2008
HBO1 histone acetylase is important for DNA replication licensing. In human cells, HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin.
Miotto, Benoit, Struhl, Kevin
openaire   +4 more sources

Dynamic recruitment of licensing factor Cdt1 to sites of DNA damage [PDF]

Journal of Cell Science, 2011
For genomic integrity to be maintained, the cell cycle and DNA damage responses must be linked. Cdt1, a G1-specific cell-cycle factor, is targeted for proteolysis by the Cul4-Ddb1Cdt2 ubiquitin ligase following DNA damage. Using a laser nanosurgery microscope to generate spatially restricted DNA damage within the living cell nucleus, we show that Cdt1 ...
Roukos, V., Kinkhabwala, A., Colombelli, J., Kotsantis, P., Taraviras, S., Nishitani, H., Stelzer, E., Bastiaens, P., Lygerou, Z.   +8 more
openaire   +5 more sources

Cdt1 revisited: complex and tight regulation during the cell cycle and consequences of deregulation in mammalian cells

Cell Division, 2006
In eukaryotic cells, replication of genomic DNA initiates from multiple replication origins distributed on multiple chromosomes. To ensure that each origin is activated precisely only once during each S phase, a system has evolved which features periodic
Fujita Masatoshi
doaj   +2 more sources

MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis [PDF]

Nature Communications
The eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre ...
Sarah V. Faull, Marta Barbon, Audrey Mossler, Zuanning Yuan, Lin Bai, L. Maximilian Reuter, Alberto Riera, Christian Winkler, Indiana Magdalou, Matthew Peach, Huilin Li, Christian Speck   +11 more
doaj   +2 more sources

Stabilisation of half MCM ring by Cdt1 during DNA insertion [PDF]

Nature Communications, 2021
During pre-Replication Complex, eukaryotic cells load two MCMs into a head-to-head Double Hexamer around duplex DNA (DH). Here the authors preRC assembly assay with purified proteins to reveal insights into S. cerevisiae’s first steps that lead to the DH
Marina Guerrero-Puigdevall, Narcis Fernandez-Fuentes, Jordi Frigola   +2 more
doaj   +4 more sources

Unidirectional MCM translocation away from ORC drives origin licensing [PDF]

Nature Communications
The MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. ATP binding supports formation of the ORC-Cdc6-Cdt1-MCM (OCCM) helicase-recruitment complex where ORC ...
Agata Butryn, Julia F. Greiwe, Alessandro Costa   +2 more
doaj   +2 more sources

A novel homozygous intronic variant in CDT1 that alters splicing causes Meier–Gorlin syndrome, and a review of published mutations and growth hormone treatments [PDF]

Orphanet Journal of Rare Diseases
Background Meier–Gorlin syndrome (MGORS) is a rare autosomal inherited form of primordial dwarfism. Pathogenic variants in 13 genes involved in DNA replication initiation have been identified in this disease, but homozygous intronic variants have never ...
Qing Li, Yichi Wu, Fucheng Meng, Zhuxi Li, Di Zhan, Xiaoping Luo   +5 more
doaj   +2 more sources

Novel Phosphorylation Sites on Cdt1 Regulate Its Activity

, 2016
Origin licensing is a process responsible for preparing the genome for replication during the cell cycle. The overexpression of chromatin licensing and DNA replication factor 1 (Cdt1), an origin licensing protein, is toxic to cells because it induces re-replication of DNA.
Cole, Yasemin
openaire   +3 more sources

Small Molecule Inhibitor Targeting CDT1/Geminin Protein Complex Promotes DNA Damage and Cell Death in Cancer Cells

Frontiers in Pharmacology, 2022
DNA replication initiation requires the loading of MCM2-7 complexes at the origins of replication during G1. Replication licensing renders chromatin competent for DNA replication and its tight regulation is essential to prevent aberrant DNA replication ...
Nikolaos Karantzelis, Michalis Petropoulos, Valeria De Marco, David A. Egan, Alexander Fish, Evangelos Christodoulou, David W. Will, Joe D. Lewis, Anastassis Perrakis, Zoi Lygerou, Stavros Taraviras   +10 more
doaj   +1 more source