Results 41 to 50 of about 5,745 (188)

Distinct and sequential re-replication barriers ensure precise genome duplication.

PLoS Genetics, 2020
Achieving complete and precise genome duplication requires that each genomic segment be replicated only once per cell division cycle. Protecting large eukaryotic genomes from re-replication requires an overlapping set of molecular mechanisms that prevent
Yizhuo Zhou, Pedro N Pozo, Seeun Oh, Haley M Stone, Jeanette Gowen Cook   +4 more
doaj   +1 more source

DDB1 Maintains Genome Integrity through Regulation of Cdt1 [PDF]

Molecular and Cellular Biology, 2006
DDB1, a component of a Cul4A ubiquitin ligase complex, promotes nucleotide excision repair (NER) and regulates DNA replication. We have investigated the role of human DDB1 in maintaining genome stability. DDB1-depleted cells accumulate DNA double-strand breaks in widely dispersed regions throughout the genome and have activated ATM and ATR cell cycle ...
Courtney A, Lovejoy, Kimberli, Lock, Ashwini, Yenamandra, David, Cortez   +3 more
openaire   +2 more sources

In Vivo DNA Re-replication Elicits Lethal Tissue Dysplasias

Cell Reports, 2017
Summary: Mammalian DNA replication origins are “licensed” by the loading of DNA helicases, a reaction that is mediated by CDC6 and CDT1 proteins. After initiation of DNA synthesis, CDC6 and CDT1 are inhibited to prevent origin reactivation and DNA ...
Sergio Muñoz, Sabela Búa, Sara Rodríguez-Acebes, Diego Megías, Sagrario Ortega, Alba de Martino, Juan Méndez   +6 more
doaj   +1 more source

Geminin Stabilizes Cdt1 during Meiosis in Xenopus Oocytes [PDF]

Journal of Biological Chemistry, 2009
During the mitotic cell cycle, Geminin can act both as a promoter and inhibitor of initiation of DNA replication. As a promoter, Geminin stabilizes Cdt1 and facilitates its accumulation leading to the assembly of the pre-replication complex on DNA. As an inhibitor, Geminin prevents Cdt1 from loading the mini-chromosome maintenance complex onto pre ...
Yadushyla, Narasimhachar, Martine, Coué
openaire   +2 more sources

The proliferation of atypical hepatocytes and CDT1 expression in noncancerous tissue are associated with the postoperative recurrence of hepatocellular carcinoma

Scientific Reports, 2022
Recently, we reported that extent of proliferation of atypical hepatocytes (atypical hepatocytes) was most important histological risk factor for development of hepatocellular carcinoma (HCC) from chronic hepatitis C or liver cirrhosis. Here, we aimed to
Mitsuhiko Moriyama, Tatsuo Kanda, Yutaka Midorikawa, Hiroshi Matsumura, Ryota Masuzaki, Hitomi Nakamura, Masahiro Ogawa, Shunichi Matsuoka, Toshikatu Shibata, Motomi Yamazaki, Kazumichi Kuroda, Hisashi Nakayama, Tokio Higaki, Kazunori Kanemaru, Toshio Miki, Masahiko Sugitani, Tadatoshi Takayama   +16 more
doaj   +1 more source

MCM9 Binds Cdt1 and Is Required for the Assembly of Prereplication Complexes [PDF]

Molecular Cell, 2008
Prereplication complexes (pre-RCs) define potential origins of DNA replication and allow the recruitment of the replicative DNA helicase MCM2-7. Here, we characterize MCM9, a member of the MCM2-8 family. We demonstrate that MCM9 binds to chromatin in an ORC-dependent manner and is required for the recruitment of the MCM2-7 helicase onto chromatin.
Lutzmann, Malik, Méchali, Marcel
openaire   +3 more sources

The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore–microtubule coupling unit [PDF]

, 2023
It is known that microtubule-binding proteins including the Ska1 complex and the DNA replication licensing factor, Cdt1, enable the kinetochore-localized Ndc80 complex to form robust kinetochore-microtubule attachments.
McKenney, Richard J, Wang, Annie Y, Varma, Dileep, Shivani Agarwal, Dileep Varma, Agarwal, Shivangi, Rahi, Amit, Amit Rahi, Annie Y. Wang, Richard J. McKenney, Agarwal, Shivani, Kristen M. Vosberg, Chakraborty, Manas, Manas Chakraborty, Shivangi Agarwal, Vosberg, Kristen M   +15 more
core   +1 more source

SPOP mutation induces replication over-firing by impairing Geminin ubiquitination and triggers replication catastrophe upon ATR inhibition

Nature Communications, 2021
Geminin-Cdt1 plays essential roles in the regulation of DNA replication. Here the authors reveal that the CULLIN3 E3 ubiquitin ligase adaptor protein SPOP prevents DNA replication over-firing and genome instability by affecting Geminin ubiquitination.
Jian Ma, Qing Shi, Gaofeng Cui, Haoyue Sheng, Maria Victoria Botuyan, Yingke Zhou, Yuqian Yan, Yundong He, Liguo Wang, Yuzhuo Wang, Georges Mer, Dingwei Ye, Chenji Wang, Haojie Huang   +13 more
doaj   +1 more source

Cdt1 overexpression drives colorectal carcinogenesis through origin overlicensing and DNA damage

, 2023
Chromatin licensing and DNA replication factor 1 (CDT1), a protein of the pre-replicative complex, is essential for loading the minichromosome maintenance complex (MCM) helicases onto the origins of DNA replication.
Champeris Tsaniras, S., Maxouri, S., Anastassiadis, K., Lygerou, Z., Roukos, V., Petropoulos, M., Kalogeropoulou, A., Taraviras, S., Danalatos, I.-R., Dionellis, V.S., Ioannidis, K., Karamichali, A., Delinasios, G.J., Halazonetis, T.D., Nikou, S., Obst, M., Naumann, R., Gorgoulis, V.G., Bravou, V.   +18 more
core   +2 more sources

The Impact of Deleting Stem-Loop 1 of Epstein–Barr Virus-Encoded RNA 1 on Cell Proliferation

Viruses, 2022
Epstein–Barr virus-encoded RNAs (EBERs) are two small, noncoding, structurally conserved transcripts, constitutively expressed at >106 copies per EBV-infected cell. They have been shown to drive cell growth.
Zubaida Hassan, Pretty S. Philip, Gulfaraz Khan   +2 more
doaj   +1 more source