Results 41 to 50 of about 6,157 (185)

Cdt1 throws kinetochore–microtubule attachments for a loop [PDF]

Nature Cell Biology, 2012
The Ndc80 complex links spindle microtubules to the kinetochore to ensure the proper segregation of chromosomes during mitosis. Analysis of the replication licensing factor Cdt1 during mitosis now reveals a cooperative role with the Ndc80 complex in establishing stable microtubule attachments to the spindle.
Daniel R. Matson, P. Todd Stukenberg
openaire   +1 more source

In Vivo DNA Re-replication Elicits Lethal Tissue Dysplasias

Cell Reports, 2017
Summary: Mammalian DNA replication origins are “licensed” by the loading of DNA helicases, a reaction that is mediated by CDC6 and CDT1 proteins. After initiation of DNA synthesis, CDC6 and CDT1 are inhibited to prevent origin reactivation and DNA ...
Sergio Muñoz, Sabela Búa, Sara Rodríguez-Acebes, Diego Megías, Sagrario Ortega, Alba de Martino, Juan Méndez   +6 more
doaj   +1 more source

Cdt1 revisited: complex and tight regulation during the cell cycle and consequences of deregulation in mammalian cells

Cell Division, 2006
In eukaryotic cells, replication of genomic DNA initiates from multiple replication origins distributed on multiple chromosomes. To ensure that each origin is activated precisely only once during each S phase, a system has evolved which features periodic
Fujita Masatoshi
doaj   +1 more source

The proliferation of atypical hepatocytes and CDT1 expression in noncancerous tissue are associated with the postoperative recurrence of hepatocellular carcinoma

Scientific Reports, 2022
Recently, we reported that extent of proliferation of atypical hepatocytes (atypical hepatocytes) was most important histological risk factor for development of hepatocellular carcinoma (HCC) from chronic hepatitis C or liver cirrhosis. Here, we aimed to
Mitsuhiko Moriyama, Tatsuo Kanda, Yutaka Midorikawa, Hiroshi Matsumura, Ryota Masuzaki, Hitomi Nakamura, Masahiro Ogawa, Shunichi Matsuoka, Toshikatu Shibata, Motomi Yamazaki, Kazumichi Kuroda, Hisashi Nakayama, Tokio Higaki, Kazunori Kanemaru, Toshio Miki, Masahiko Sugitani, Tadatoshi Takayama   +16 more
doaj   +1 more source

Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point‐of‐Care Detection of Viral RNA

Angewandte Chemie, EarlyView.
A new exponential RCA (E‐RCA) system was developed and coupled to a toehold system for release of a bridging DNA, allowing for lateral flow detection. The E‐RCA method uses a secondary primer (P2) that binds RCA products (RCAP) to generate extended P2 products that can re‐prime a partially self‐complementary circular template, generating a complete ...
Amal Mathai, Monsur Ali, Yingfu Li, John D. Brennan   +3 more
wiley   +2 more sources

MCM9 Binds Cdt1 and Is Required for the Assembly of Prereplication Complexes [PDF]

Molecular Cell, 2008
Prereplication complexes (pre-RCs) define potential origins of DNA replication and allow the recruitment of the replicative DNA helicase MCM2-7. Here, we characterize MCM9, a member of the MCM2-8 family. We demonstrate that MCM9 binds to chromatin in an ORC-dependent manner and is required for the recruitment of the MCM2-7 helicase onto chromatin.
Lutzmann, Malik, Méchali, Marcel
openaire   +3 more sources

SPOP mutation induces replication over-firing by impairing Geminin ubiquitination and triggers replication catastrophe upon ATR inhibition

Nature Communications, 2021
Geminin-Cdt1 plays essential roles in the regulation of DNA replication. Here the authors reveal that the CULLIN3 E3 ubiquitin ligase adaptor protein SPOP prevents DNA replication over-firing and genome instability by affecting Geminin ubiquitination.
Jian Ma, Qing Shi, Gaofeng Cui, Haoyue Sheng, Maria Victoria Botuyan, Yingke Zhou, Yuqian Yan, Yundong He, Liguo Wang, Yuzhuo Wang, Georges Mer, Dingwei Ye, Chenji Wang, Haojie Huang   +13 more
doaj   +1 more source

Direct binding of Cdt2 to PCNA is important for targeting the CRL4 Cdt2 E3 ligase activity to Cdt1

Life Science Alliance, 2018
The C-terminal end of Cdt2 contains a PIP box for binding to PCNA to promote CRL4 Cdt2 function, creating a new paradigm where the substrate receptor and substrates bind to a common multivalent docking platform for ubiquitination. The CRL4 Cdt2 ubiquitin
Akiyo Hayashi, Nickolaos Nikiforos Giakoumakis, Tatjana Heidebrecht, Takashi Ishii, Andreas Panagopoulos, Christophe Caillat, Michiyo Takahara, Richard G Hibbert, Naohiro Suenaga, Magda Stadnik-Spiewak, Tatsuro Takahashi, Yasushi Shiomi, Stavros Taraviras, Eleonore von Castelmur, Zoi Lygerou, Anastassis Perrakis, Hideo Nishitani   +16 more
doaj   +1 more source

The Impact of Deleting Stem-Loop 1 of Epstein–Barr Virus-Encoded RNA 1 on Cell Proliferation

Viruses, 2022
Epstein–Barr virus-encoded RNAs (EBERs) are two small, noncoding, structurally conserved transcripts, constitutively expressed at >106 copies per EBV-infected cell. They have been shown to drive cell growth.
Zubaida Hassan, Pretty S. Philip, Gulfaraz Khan   +2 more
doaj   +1 more source

Translating whole‐genome doubling into precision medicine in cancer

Molecular Oncology, EarlyView.
Whole‐genome doubling creates a WGD‐positive tumor state characterized by persistent chromosomal instability, karyotypic diversification, and cellular stress. These same biological pressures drive aggressive tumor evolution while exposing therapeutic vulnerabilities, providing a rationale for WGD‐informed precision medicine. Whole‐genome doubling (WGD)
Sejung Lee, Junghyeok Lim, Jinhyuk Bhin
wiley   +1 more source