Results 61 to 70 of about 5,045 (159)

Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system. [PDF]

PLoS ONE, 2013
A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system.
Michio Tomura, Asako Sakaue-Sawano, Yoshiko Mori, Mitsuyo Takase-Utsugi, Akihiro Hata, Kenji Ohtawa, Osami Kanagawa, Atsushi Miyawaki   +7 more
doaj   +1 more source

Cells Dynamically Adapt Their Nuclear Volumes and Proliferation Rates During Single to Multicellular Transitions

Advanced Science, Volume 13, Issue 31, 4 June 2026.
It is currently not well understood how cells regulate basic properties, e.g., volume and mechanics within dense multicellular environments like tumors. Here, we show that different cell types of cancer and also normal cells largely decrease their nuclear and cellular volumes in emerging cell clusters and that this is partly driven by cell cycle shifts.
Vaibhav Mahajan, Keshav Gajendra Babu, Markus Mukenhirn, Antje Garside, Vinita Ajit Kini, Trishla Adhikari, Timon Beck, Byung Ho Lee, Kyoohyun Kim, Carsten Werner, Raimund Schlüßler, Alf Honigmann, Sebastian Aland, Anna Verena Taubenberger   +13 more
wiley   +1 more source

Jnk2 effects on tumor development, genetic instability and replicative stress in an oncogene-driven mouse mammary tumor model. [PDF]

PLoS ONE, 2010
Oncogenes induce cell proliferation leading to replicative stress, DNA damage and genomic instability. A wide variety of cellular stresses activate c-Jun N-terminal kinase (JNK) proteins, but few studies have directly addressed the roles of JNK isoforms ...
Peila Chen, Jamye F O'Neal, Nancy D Ebelt, Michael A Cantrell, Shreya Mitra, Azadeh Nasrazadani, Tracy L Vandenbroek, Lynn E Heasley, Carla L Van Den Berg   +8 more
doaj   +1 more source

HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1 [PDF]

Genes & Development, 2008
HBO1 histone acetylase is important for DNA replication licensing. In human cells, HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin.
Miotto, Benoit, Struhl, Kevin
openaire   +3 more sources

Methodology to Create Auxin-Inducible Degron Tagging System to Control Expression of a Target Protein in Mammalian Cell Lines

Bio-Protocol
The auxin-inducible degron (AID) system is a versatile tool in cell biology and genetics, enabling conditional protein regulation through auxin-induced degradation. Integrating CRISPR/Cas9 with AID expedites tagging and depletion of a required protein in
Amit Rahi, Deepika Sodhi, Christine Magdongon, Rajina Shakya, Dileep Varma   +4 more
doaj   +1 more source

Pitfalls and missing links in current understanding of 4D genomes

Quantitative Biology, Volume 14, Issue 2, June 2026.
Abstract The spatial and temporal organization of the genome—collectively termed the 4D genome—is pivotal for regulating gene expression, maintaining genome stability, and guiding development. The convergence of chromosome conformation capture technologies, super‐resolution microscopy, and single‐cell epigenomics has transformed our understanding of ...
Michael Q. Zhang
wiley   +1 more source

Cdt1 Variants Offer Novel Insights Into Cdt1‐MCM Interactions and an Unexpected Mechanism for Cyclin A to Block DNA Re‐Replication

The FASEB Journal, 2018
The correct execution of DNA replication is critical for normal cell proliferation. The earliest step in DNA replication is known as origin licensing. Origins are licensed by the loading of MCM complexes, an event that is restricted to G1 phase.
Pedro Nicolas Pozo, Yasemin Cole, Jacob Matson, Yizhuo Zhuo, Brenda Temple, Jeanette Gowen Cook   +5 more
openaire   +1 more source

Multi‐Scale Mapping of Gene Expression from Whole‐slide Images for Identifying Phenotype‐Associated Subpopulations

Advanced Science, Volume 13, Issue 26, 8 May 2026.
BiSCALE: A pathology‐driven deep learning framework for multi‐scale gene expression prediction from whole‐slide images. It accurately infers bulk and near‐cellular spot‐level expression, links predictions to clinical phenotypes, identifies disease‐associated niches, and enables applications in risk stratification and cell‐identity annotation, providing
Hailong Zheng, Jiajing Xie, Luqi Wang, Hailong Xie, Tong Zhi, Yujia Guo, Bofeng Zhu, Dong Wang, Yu Chen   +8 more
wiley   +1 more source

Analysis of a temperature‐sensitive mutation in Uba1: Effects of the click reaction on subsequent immunolabeling of proteins involved in DNA replication

FEBS Open Bio, 2015
In our previous study, a Met‐to‐Ile substitution at amino acid 256 in the catalytic domain of Uba1 was determined in temperature‐sensitive CHO‐K1 mutant tsTM3 cells, which exhibited chromosomal instability and cell‐cycle arrest in the S to G2 phases with
Kimihiko Sugaya, Yoshie Ishihara, Sonoe Inoue   +2 more
doaj   +1 more source

A new class of disordered elements controls DNA replication through initiator self-assembly

eLife, 2019
The initiation of DNA replication in metazoans occurs at thousands of chromosomal sites known as origins. At each origin, the Origin Recognition Complex (ORC), Cdc6, and Cdt1 co-assemble to load the Mcm2-7 replicative helicase onto chromatin.
Matthew W Parker, Maren Bell, Mustafa Mir, Jonchee A Kao, Xavier Darzacq, Michael R Botchan, James M Berger   +6 more
doaj   +1 more source