Results 71 to 80 of about 6,157 (185)

Ectopic expression of Cdt1 lowers the G1 arrest response.

, 2015
A. Extra copy of Cdt1 expressing cells have more MCM2-7 on the chromatin. Control (293) and Cdt1-3NLSmyc expressing HEK293 cells (Cdt1) were arrested in M phase and released with (M-UV) or without [(-)UV] UV irradiation (60 J/m2).
Takeshi Maeda (304955), Michiyo Takahara (709220), Kohei Nukina (709219), Yasushi Shiomi (304958), Hideo Nishitani (304959), Hiroki Sakaguchi (304952), Masayuki Morino (304956)   +6 more
core   +1 more source

Cdt1 overexpression drives colorectal carcinogenesis through origin overlicensing and DNA damage

, 2023
Chromatin licensing and DNA replication factor 1 (CDT1), a protein of the pre-replicative complex, is essential for loading the minichromosome maintenance complex (MCM) helicases onto the origins of DNA replication.
Champeris Tsaniras, S., Maxouri, S., Anastassiadis, K., Lygerou, Z., Roukos, V., Petropoulos, M., Kalogeropoulou, A., Taraviras, S., Danalatos, I.-R., Dionellis, V.S., Ioannidis, K., Karamichali, A., Delinasios, G.J., Halazonetis, T.D., Nikou, S., Obst, M., Naumann, R., Gorgoulis, V.G., Bravou, V.   +18 more
core   +1 more source

Jnk2 effects on tumor development, genetic instability and replicative stress in an oncogene-driven mouse mammary tumor model. [PDF]

PLoS ONE, 2010
Oncogenes induce cell proliferation leading to replicative stress, DNA damage and genomic instability. A wide variety of cellular stresses activate c-Jun N-terminal kinase (JNK) proteins, but few studies have directly addressed the roles of JNK isoforms ...
Peila Chen, Jamye F O'Neal, Nancy D Ebelt, Michael A Cantrell, Shreya Mitra, Azadeh Nasrazadani, Tracy L Vandenbroek, Lynn E Heasley, Carla L Van Den Berg   +8 more
doaj   +1 more source

Methodology to Create Auxin-Inducible Degron Tagging System to Control Expression of a Target Protein in Mammalian Cell Lines

Bio-Protocol
The auxin-inducible degron (AID) system is a versatile tool in cell biology and genetics, enabling conditional protein regulation through auxin-induced degradation. Integrating CRISPR/Cas9 with AID expedites tagging and depletion of a required protein in
Amit Rahi, Deepika Sodhi, Christine Magdongon, Rajina Shakya, Dileep Varma   +4 more
doaj   +1 more source

Cdt1 stabilizes an open MCM ring for helicase loading

, 2020
ORC, Cdc6 and Cdt1 act together to load hexameric MCM, the motor of the eukaryotic replicative helicase, into double hexamers at replication origins. Here we show that Cdt1 interacts with MCM subunits Mcm2, 4 and 6, which both destabilizes the Mcm2-5 ...
Valerie E Pye (8128395), Ludovic Renault (469927), Jordi Frigola (2345797), Peter Cherepanov (369), Alessandro Costa (2349019), Jun He (46556), Kerstin Kinkelin (9233102), John FX Diffley (8124071), Dirk Remus (9233105), Max E Douglas (9108548)   +9 more
core  

Caffeine inhibits UV-induced rapid degradation of Cdt1.

, 2013
A. Asynchronously growing HeLa cells treated with caffeine (+, 10 mM) or not (−) were UV-irradiated (50 J/m2) and collected for Western blotting with Cdt1 and Cdt2. For Cdt2 blotting, samples were run on Phos-tag gels.
Takeshi Maeda (304955), Toshihiro Takami (304953), Yasushi Shiomi (304958), Yoshinori Yasutani (304954), Hideo Nishitani (304959), Hiroki Sakaguchi (304952), Takashi Ishii (304957), Masayuki Morino (304956)   +7 more
core   +1 more source

Cdt1 variants reveal unanticipated aspects of interactions with Cyclin/CDK and MCM important for normal genome replication [PDF]

, 2018
The earliest step in DNA replication is origin licensing which is the DNA loading of MCM helicase complexes. The Cdt1 protein is essential for MCM loading during G1 phase of the cell cycle, yet the mechanism of Cdt1 function is still incompletely ...
Jeanette Gowen Cook, Pedro N. Pozo, Yasemin Cole, Katarzyna M. Kedziora, Jacob P. Matson, Brenda Temple, Gavin D. Grant   +6 more
core   +2 more sources

O‐Glycosylation of E‐Cadherin Induced by Endoplasmic Reticulum Stress Negatively Regulates Cell Polarity and Proliferation in Acute Kidney Injury

Journal of Biochemical and Molecular Toxicology, Volume 40, Issue 8, August 2026.
ER stress could cause the O‐glycosylation of E‐cadherin in RTECs, leading to dysregulation of cell polarity and proliferation. ER stress inhibitors alleviate kidney injury and promote the recovery of kidney function. These findings provide new molecular targets and intervention strategies for the clinical treatment of AKI. ABSTRACT The loss of polarity
Yingjia Zhang, Weidong Zhang, Qingwu Liao, Zhaoxing Sun, Weize Chen, Xiaoqiang Ding, Shuan Zhao   +6 more
wiley   +1 more source

A new class of disordered elements controls DNA replication through initiator self-assembly

eLife, 2019
The initiation of DNA replication in metazoans occurs at thousands of chromosomal sites known as origins. At each origin, the Origin Recognition Complex (ORC), Cdc6, and Cdt1 co-assemble to load the Mcm2-7 replicative helicase onto chromatin.
Matthew W Parker, Maren Bell, Mustafa Mir, Jonchee A Kao, Xavier Darzacq, Michael R Botchan, James M Berger   +6 more
doaj   +1 more source

Cdt1 modulates kinetochore-microtubule attachment stabilization via an Aurora B kinase-dependent mechanism

, 2017
Robust kinetochore-microtubule (kMT) attachment is critical for accurate chromosome segregation. G2/M-specific depletion of human Cdt1 that localizes to kinetochores in an Ndc80 complex-dependent manner, leads to abnormal kMT attachments and mitotic ...
Aussie Suzuki, Richard J. McKenney, Yizhuo Zhou, Dileep Varma, Shivangi Agarwal, Kyle Paul Smith   +5 more
core   +1 more source