Results 71 to 80 of about 5,745 (188)

Morphofunctional Heterogeneity and Plasticity of Glioblastoma Cells Induced to Senescence by Temozolomide

Aging Cell, Volume 25, Issue 4, April 2026.
Glioblastoma cells induced to senescence by Temozolomide are heterogeneous, with the emergence of two main states, named E‐state and F‐state, which differ in cellular morphometry, survival mechanisms, and sensitivity to autophagy modulators and senolytics. These states are dynamic, with the occurrence of morphofunctional transitions.
Solon Andrades da Rosa, Henrique Quaiato de Oliveira, Laura Boose de Mendonça, Nicole Borgmann de Oliveira, Mariane da Cunha Jaeger, Luiza Cherobini Pereira, Fernanda Dittrich Pinto Oliveira, Debora Santos‐Sousa, Fernanda Saez‐Calazans, Luana Lenz, Melike Lakadamyali, Guido Lenz, Eduardo Cremonese Filippi‐Chiela   +12 more
wiley   +1 more source

Cdt1 throws kinetochore–microtubule attachments for a loop [PDF]

Nature Cell Biology, 2012
The Ndc80 complex links spindle microtubules to the kinetochore to ensure the proper segregation of chromosomes during mitosis. Analysis of the replication licensing factor Cdt1 during mitosis now reveals a cooperative role with the Ndc80 complex in establishing stable microtubule attachments to the spindle.
Daniel R. Matson, P. Todd Stukenberg
openaire   +1 more source

Cdt1 modulates kinetochore-microtubule attachment stabilization via an Aurora B kinase-dependent mechanism

, 2017
Robust kinetochore-microtubule (kMT) attachment is critical for accurate chromosome segregation. G2/M-specific depletion of human Cdt1 that localizes to kinetochores in an Ndc80 complex-dependent manner, leads to abnormal kMT attachments and mitotic ...
Aussie Suzuki, Richard J. McKenney, Yizhuo Zhou, Dileep Varma, Shivangi Agarwal, Kyle Paul Smith   +5 more
core   +1 more source

Cdt1 stabilizes an open MCM ring for helicase loading

, 2020
ORC, Cdc6 and Cdt1 act together to load hexameric MCM, the motor of the eukaryotic replicative helicase, into double hexamers at replication origins. Here we show that Cdt1 interacts with MCM subunits Mcm2, 4 and 6, which both destabilizes the Mcm2-5 ...
Valerie E Pye (8128395), Ludovic Renault (469927), Jordi Frigola (2345797), Peter Cherepanov (369), Alessandro Costa (2349019), Jun He (46556), Kerstin Kinkelin (9233102), John FX Diffley (8124071), Dirk Remus (9233105), Max E Douglas (9108548)   +9 more
core  

Repression of Nascent Strand Elongation by Deregulated Cdt1 during DNA Replication in Xenopus Egg Extracts

, 2009
Excess Cdt1 reportedly induces rereplication of chromatin in cultured cells and Xenopus egg extracts, suggesting that the regulation of Cdt1 activity by cell cycle-dependent proteolysis and expression of the Cdt1 inhibitor geminin is crucial for the ...
Enomoto, T, Ayako Aoki, Masayuki Seki, Tada, S, Tsuyama, T, Saori Watanabe, Aoki, A, Takemi Enomoto, Takashi Tsuyama, Yunje Cho, Shusuke Tada, Watanabe, S, Cho, Y, Seki, M   +13 more
core   +1 more source

Gene Amplification in Tumor Cells: Developed De Novo or Adopted from Stem Cells

Cells, 2022
Gene amplifications have been known for several decades as physiological processes in amphibian and flies, e.g., during eggshell development in Drosophila and as part of pathological processes in humans, specifically in tumors and drug-resistant cells ...
Ulrike Fischer, Eckart Meese
doaj   +1 more source

Methodology to Create Auxin-Inducible Degron Tagging System to Control Expression of a Target Protein in Mammalian Cell Lines

Bio-Protocol
The auxin-inducible degron (AID) system is a versatile tool in cell biology and genetics, enabling conditional protein regulation through auxin-induced degradation. Integrating CRISPR/Cas9 with AID expedites tagging and depletion of a required protein in
Amit Rahi, Deepika Sodhi, Christine Magdongon, Rajina Shakya, Dileep Varma   +4 more
doaj   +1 more source

Analysis of a temperature‐sensitive mutation in Uba1: Effects of the click reaction on subsequent immunolabeling of proteins involved in DNA replication

FEBS Open Bio, 2015
In our previous study, a Met‐to‐Ile substitution at amino acid 256 in the catalytic domain of Uba1 was determined in temperature‐sensitive CHO‐K1 mutant tsTM3 cells, which exhibited chromosomal instability and cell‐cycle arrest in the S to G2 phases with
Kimihiko Sugaya, Yoshie Ishihara, Sonoe Inoue   +2 more
doaj   +1 more source

A new class of disordered elements controls DNA replication through initiator self-assembly

eLife, 2019
The initiation of DNA replication in metazoans occurs at thousands of chromosomal sites known as origins. At each origin, the Origin Recognition Complex (ORC), Cdc6, and Cdt1 co-assemble to load the Mcm2-7 replicative helicase onto chromatin.
Matthew W Parker, Maren Bell, Mustafa Mir, Jonchee A Kao, Xavier Darzacq, Michael R Botchan, James M Berger   +6 more
doaj   +1 more source

The Metacarpophalangeal Pattern Profile: An Old Method With New Insights Into the Evaluation of Short Stature

American Journal of Human Biology, Volume 38, Issue 2, February 2026.
ABSTRACT Objective To characterize the metacarpophalangeal pattern profile (MCPP) of healthy children and adolescents from São Paulo, Brazil, and to establish percentile curves by chronological age (CA), bone age (BA), and sex using the LMS method. Additionally, to compare these findings with previous population‐based data and to apply the derived ...
Marcelo Damaso Maruichi, Bruno Telma Destailleur, Giulia Maesta Apelbaum, Carlos Alberto Longui, Cristiane Kochi   +4 more
wiley   +1 more source