Results 51 to 60 of about 6,157 (185)

Kinetochore–microtubule coupling mechanisms mediated by the Ska1 complex and Cdt1

Essays in Biochemistry, 2020
Abstract The faithful segregation of duplicated sister chromatids rely on the remarkable ability of kinetochores to sustain stable load bearing attachments with the dynamic plus ends of kinetochore–microtubules (kMTs). The outer layer of the kinetochore recruits several motor and non-motor microtubule-associated proteins (MAPs) that help
Amit Rahi, Manas Chakraborty, Kristen Vosberg, Dileep Varma   +3 more
openaire   +3 more sources

The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore–microtubule coupling unit [PDF]

, 2023
It is known that microtubule-binding proteins including the Ska1 complex and the DNA replication licensing factor, Cdt1, enable the kinetochore-localized Ndc80 complex to form robust kinetochore-microtubule attachments.
McKenney, Richard J, Wang, Annie Y, Varma, Dileep, Shivani Agarwal, Dileep Varma, Agarwal, Shivangi, Rahi, Amit, Amit Rahi, Annie Y. Wang, Richard J. McKenney, Agarwal, Shivani, Kristen M. Vosberg, Chakraborty, Manas, Manas Chakraborty, Shivangi Agarwal, Vosberg, Kristen M   +15 more
core   +1 more source

A Temporary Pause in the Replication Licensing Restriction Leads to Rereplication during Early Human Cell Differentiation

Cells, 2022
Gene amplifications in amphibians and flies are known to occur during development and have been well characterized, unlike in mammalian cells, where they are predominantly investigated as an attribute of tumors.
Marie Minet, Masood Abu-Halima, Yiqing Du, Julia Doerr, Christina Isted, Nicole Ludwig, Andreas Keller, Eckart Meese, Ulrike Fischer   +8 more
doaj   +1 more source

Transposable Element–Driven PIEZO Mutation Enhances Locust Flight in Plateau Hypoxia

Advanced Science, EarlyView.
Why transposable elements (TEs) persisted or expanded in genomes remains a mystery. Using integrated analysis of TE macro‐ and microevolution in locusts, our results showed that thousands of TE insertions promoted widespread adaptive variation. Subfamilies of candidate adaptive TEs amplified and reshaped species‐level genomic architecture.
Xuanzhao Li, Ying Liu, Longsheng Xing, Xianliang Huang, Yingming Sun, Lei Yue, Yuze Zhang, Huilong Du, Bing Chen   +8 more
wiley   +1 more source

Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation. [PDF]

, 2011
Cdt1 plays a critical role in DNA replication regulation by controlling licensing. In Metazoa, Cdt1 is regulated by CRL4(Cdt2)-mediated ubiquitylation, which is triggered by DNA binding of proliferating cell nuclear antigen (PCNA).
Rachel S. Deegan, Shepherd, ME, Salguero, I, Kearsey, Stephen, Deegan, RS, Guarino Almeida, Estrella, Shepherd, Marianne E. A., Guarino, E, Hui Hua, Kearsey, Stephen E., Kearsey, SE, Salguero, Israel, Deegan, Rachel S., Hua, H, Estrella Guarino, Hua, Hui, Marianne E. A. Shepherd, Israel Salguero, Stephen E. Kearsey   +18 more
core   +1 more source

Modulation of Mcm2–7 activity by Cdt1

The FASEB Journal, 2013
Genome duplication occurs once and only once in each cell cycle. During G1 phase, pre‐replicative complexes (pre‐RC), composed of ORC, Cdc6, Cdt1 and Mcm2–7, are assembled at replication origins. Mcm2–7 is the replicative helicase in eukaryotic cells; however, it does not unwind DNA in the pre‐RC.
Lance F DaSilva, Tomasz Kolaczyk, Xiaoli Ma, Megan J Davey   +3 more
openaire   +1 more source

DNA damaging chemical-induced Cdt1 degradation.

, 2013
A and B. DNA damage-inducing reagent MMS or zeocin-induced PCNA-CRL4Cdt2 mediated degradation of Cdt1. 293T cells were treated with MMS (0.5 mM) or zeocin (2 mg/ml) for 1 h, and Cdt1 levels and γH2AX levels were examined (A).
Takeshi Maeda (304955), Toshihiro Takami (304953), Yasushi Shiomi (304958), Yoshinori Yasutani (304954), Hideo Nishitani (304959), Hiroki Sakaguchi (304952), Takashi Ishii (304957), Masayuki Morino (304956)   +7 more
core   +1 more source

Cdt1 stabilizes kinetochore–microtubule attachments via an Aurora B kinase–dependent mechanism [PDF]

, 2018
Robust kinetochore-microtubule (kMT) attachment is critical for accurate chromosome segregation. G2/M-specific depletion of human Cdt1 that localizes to kinetochores in an Ndc80 complex-dependent manner leads to abnormal kMT attachments and mitotic ...
Aussie Suzuki, McKenney, Richard J, Richard J. McKenney, Yizhuo Zhou, Varma, Dileep, Zhou, Yizhuo, Suzuki, Aussie, Smith, Kyle Paul, Dileep Varma, Shivangi Agarwal, Agarwal, Shivangi, Kyle Paul Smith   +11 more
core   +1 more source

Labeling Strategies for Monitoring Cytoskeleton Dynamics in Cultured Epithelial Cells

Cytoskeleton, EarlyView.
ABSTRACT Collective cell migration relies on coordinated cytoskeletal remodeling, yet the impact of live‐cell actin filament probes on these dynamics remains poorly characterized. Here, we systematically compared the performance and cellular effects of fluorogenic jasplakinolide‐based probes, SiR‐actin and SiR‐XActin, with the genetically encoded ...
Victoria Levario‐Diaz, Lakshmi Nyapathi‐Gopinath, Jonah Luka Voigt, Katharina Weber, Marlene Maager, Veselin Nasufovic, Elisabetta Ada Cavalcanti‐Adam   +6 more
wiley   +1 more source

Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells [PDF]

Genes to Cells, 2017
Geminin is implicated in regulation of the cell cycle and differentiation. Although loss of Geminin triggers unscheduled DNA rereplication as a result of interruption of its interaction with Cdt1 in some somatic cancer cells, whether such cell cycle regulation also operates in embryonic stem cells (ESCs) has remained unclear.
Masaki, Hosogane, Lena, Bosu, Emiko, Fukumoto, Hidetoshi, Yamada, Soichiro, Sato, Keiko, Nakayama   +5 more
openaire   +2 more sources