Results 51 to 60 of about 5,045 (159)

Cdt1 throws kinetochore–microtubule attachments for a loop [PDF]

Nature Cell Biology, 2012
The Ndc80 complex links spindle microtubules to the kinetochore to ensure the proper segregation of chromosomes during mitosis. Analysis of the replication licensing factor Cdt1 during mitosis now reveals a cooperative role with the Ndc80 complex in establishing stable microtubule attachments to the spindle.
Daniel R. Matson, P. Todd Stukenberg
openaire   +1 more source

Labeling Strategies for Monitoring Cytoskeleton Dynamics in Cultured Epithelial Cells

Cytoskeleton, EarlyView.
ABSTRACT Collective cell migration relies on coordinated cytoskeletal remodeling, yet the impact of live‐cell actin filament probes on these dynamics remains poorly characterized. Here, we systematically compared the performance and cellular effects of fluorogenic jasplakinolide‐based probes, SiR‐actin and SiR‐XActin, with the genetically encoded ...
Victoria Levario‐Diaz, Lakshmi Nyapathi‐Gopinath, Jonah Luka Voigt, Katharina Weber, Marlene Maager, Veselin Nasufovic, Elisabetta Ada Cavalcanti‐Adam   +6 more
wiley   +1 more source

An open‐source pipeline for longitudinal single‐cell tracking and cell‐cycle/migration coupling analysis for neurotherapeutic screening

Neuroprotection, EarlyView.
Integrated experimental and computational pipeline for longitudinal single‐cell tracking and cell‐cycle dynamics analysis. Schematic of the FUCCI (Fluorescence Ubiquitination‐based Cell Cycle Indicator) reporter system. The construct is driven by the CAG promoter (CMV enhancer + chicken β‐actin promoter) and encodes two fusion proteins: mKO2‐Cdt1 ...
Neha Chandra, Matthew Yang, Hailey Wang, Miroslaw Janowski, Piotr Walczak, Cedric Allier, Yajie Liang   +6 more
wiley   +1 more source

Regulation of CDC6, Geminin, and CDT1 in Human Cells that Undergo Polyploidization [PDF]

Molecular Biology of the Cell, 2002
Endomitosis is the process by which mammalian megakaryocytes become polyploid during terminal differentiation. As in other endoreplicating cells, cyclin-cdk complexes are distinctly regulated, probably to overcome the strict mechanisms that prevent rereplication in most somatic cells.
Bermejo, Rodrigo, Vilaboa, Nuria, Calés, Carmela   +2 more
openaire   +3 more sources

Phytochemicals as Multitarget Therapeutics in Pancreatic Cancer: Mechanisms, Clinical Evidence, and Translational Challenges

Phytotherapy Research, EarlyView.
Pancreatic cancer, particularly pancreatic ductal adenocarcinoma, is aggressive and often diagnosed late, making treatment challenging. This review highlights the potential of phytochemicals, such as curcumin and quercetin, as multitarget agents that influence key carcinogenic pathways. These compounds exhibit anticancer effects by inhibiting processes
Md. Rezaul Islam, Abdur Rauf, F. M. Shourav, Hanan A. Ogaly, Abdullah S. M. Aljohani, Waleed Al Abdulmonem, Kamala Badalova, Marcello Iriti   +7 more
wiley   +1 more source

DDB2 regulates DNA replication through PCNA-independent degradation of CDT2

Cell & Bioscience, 2021
Background Targeting ubiquitin-dependent proteolysis is one of the strategies in cancer therapy. CRLCDT2 and CRLDDB2 are two key E3 ubiquitin ligases involved in DNA replication and DNA damage repair.
Xiaojun Wu, Min Yu, Zhuxia Zhang, Feng Leng, Yue Ma, Ni Xie, Fei Lu   +6 more
doaj   +1 more source

Ploidy‐dependent modulation of DNA replication kinetics in Xenopus

The FEBS Journal, EarlyView.
Using an interspecies replication system with Xenopus frog species of different ploidy levels, we investigated how DNA replication adapts to polyploidization. While replication was slower in tetraploid than in diploid nuclei, dodecaploid nuclei unexpectedly replicated faster than tetraploid nuclei. Our findings show that increasing genome size does not
Hemalatha Narassimprakash, Olivier Haccard, Zhen Li, Nicolas Pollet, Kathrin Marheineke   +4 more
wiley   +1 more source

An 11-Year-Old Girl With Rare Diagnosis of Meier-Gorlin Syndrome Accompanied by Neonatal Seizure, Mental Retardation, and Attention Deficit Hyperactivity Disorder: A Case Report and Review of Literature

Acta Medica Iranica, 2022
Meier-Gorlin syndrome (MGS) is a rare autosomal recessive disorder with homozygous or heterozygous mutations in one of the five following genes (ORC1, ORC4, ORC6, CDT1, and CDC6).
Shahin Koomanaee, Setila Dalili, Seyyedeh Azade Hoseini Nouri, Seyedeh Zohreh Jalali, Manijeh Tabrizi, Ghazaleh Aldaghi, Afagh Hassanzadeh Rad   +6 more
doaj   +1 more source

O‐Glycosylation of E‐Cadherin Induced by Endoplasmic Reticulum Stress Negatively Regulates Cell Polarity and Proliferation in Acute Kidney Injury

Journal of Biochemical and Molecular Toxicology, Volume 40, Issue 8, August 2026.
ER stress could cause the O‐glycosylation of E‐cadherin in RTECs, leading to dysregulation of cell polarity and proliferation. ER stress inhibitors alleviate kidney injury and promote the recovery of kidney function. These findings provide new molecular targets and intervention strategies for the clinical treatment of AKI. ABSTRACT The loss of polarity
Yingjia Zhang, Weidong Zhang, Qingwu Liao, Zhaoxing Sun, Weize Chen, Xiaoqiang Ding, Shuan Zhao   +6 more
wiley   +1 more source

mRNA expression of the DNA replication-initiation proteins in epithelial dysplasia and squamous cell carcinoma of the tongue

BMC Cancer, 2008
Background The tongue squamous cell carcinomas (SCCs) are characterized by high mitotic activity, and early detection is desirable. Overexpression of the DNA replication-initiation proteins has been associated with dysplasia and malignancy.
Tu Zheng, Lu Yong-jun, Li Hui-jun, Feng Chong-jin, Li Jian-na, Liao Gui-qing, Liang Chun   +6 more
doaj   +1 more source