Results 51 to 60 of about 5,745 (188)

Direct binding of Cdt2 to PCNA is important for targeting the CRL4 Cdt2 E3 ligase activity to Cdt1

Life Science Alliance, 2018
The C-terminal end of Cdt2 contains a PIP box for binding to PCNA to promote CRL4 Cdt2 function, creating a new paradigm where the substrate receptor and substrates bind to a common multivalent docking platform for ubiquitination. The CRL4 Cdt2 ubiquitin
Akiyo Hayashi, Nickolaos Nikiforos Giakoumakis, Tatjana Heidebrecht, Takashi Ishii, Andreas Panagopoulos, Christophe Caillat, Michiyo Takahara, Richard G Hibbert, Naohiro Suenaga, Magda Stadnik-Spiewak, Tatsuro Takahashi, Yasushi Shiomi, Stavros Taraviras, Eleonore von Castelmur, Zoi Lygerou, Anastassis Perrakis, Hideo Nishitani   +16 more
doaj   +1 more source

A Temporary Pause in the Replication Licensing Restriction Leads to Rereplication during Early Human Cell Differentiation

Cells, 2022
Gene amplifications in amphibians and flies are known to occur during development and have been well characterized, unlike in mammalian cells, where they are predominantly investigated as an attribute of tumors.
Marie Minet, Masood Abu-Halima, Yiqing Du, Julia Doerr, Christina Isted, Nicole Ludwig, Andreas Keller, Eckart Meese, Ulrike Fischer   +8 more
doaj   +1 more source

Modulation of Mcm2–7 activity by Cdt1

The FASEB Journal, 2013
Genome duplication occurs once and only once in each cell cycle. During G1 phase, pre‐replicative complexes (pre‐RC), composed of ORC, Cdc6, Cdt1 and Mcm2–7, are assembled at replication origins. Mcm2–7 is the replicative helicase in eukaryotic cells; however, it does not unwind DNA in the pre‐RC.
Lance F DaSilva, Tomasz Kolaczyk, Xiaoli Ma, Megan J Davey   +3 more
openaire   +1 more source

Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation

Advanced Science, Volume 13, Issue 34, 19 June 2026.
Nuclear metabolism oscillates during cell cycle progression. Quantitative chromatome proteomics and imaging reveal phase‐specific dynamics of PIP5K1A and nuclear PIP2, linking phosphatidylinositol metabolism to histone methylation. This work identifies nuclear lipid metabolism as a previously unrecognized regulatory axis coordinating chromatin ...
Antoni Gañez‐Zapater, Savvas Kourtis, Camilla Reiter Elbæk, Lorena Espinar, Carolina Toro‐Márquez, Albert Coll‐Manzano, Alfredo Smiriglia, Laura García‐López, Laura Wiegand, Maria Guirola, Frédéric Fontaine, Andrea Morandi, André C. Müller, Sara Sdelci   +13 more
wiley   +1 more source

Cells Dynamically Adapt Their Nuclear Volumes and Proliferation Rates During Single to Multicellular Transitions

Advanced Science, Volume 13, Issue 31, 4 June 2026.
It is currently not well understood how cells regulate basic properties, e.g., volume and mechanics within dense multicellular environments like tumors. Here, we show that different cell types of cancer and also normal cells largely decrease their nuclear and cellular volumes in emerging cell clusters and that this is partly driven by cell cycle shifts.
Vaibhav Mahajan, Keshav Gajendra Babu, Markus Mukenhirn, Antje Garside, Vinita Ajit Kini, Trishla Adhikari, Timon Beck, Byung Ho Lee, Kyoohyun Kim, Carsten Werner, Raimund Schlüßler, Alf Honigmann, Sebastian Aland, Anna Verena Taubenberger   +13 more
wiley   +1 more source

Kinetochore–microtubule coupling mechanisms mediated by the Ska1 complex and Cdt1

Essays in Biochemistry, 2020
Abstract The faithful segregation of duplicated sister chromatids rely on the remarkable ability of kinetochores to sustain stable load bearing attachments with the dynamic plus ends of kinetochore–microtubules (kMTs). The outer layer of the kinetochore recruits several motor and non-motor microtubule-associated proteins (MAPs) that help
Amit Rahi, Manas Chakraborty, Kristen Vosberg, Dileep Varma   +3 more
openaire   +3 more sources

DDB2 regulates DNA replication through PCNA-independent degradation of CDT2

Cell & Bioscience, 2021
Background Targeting ubiquitin-dependent proteolysis is one of the strategies in cancer therapy. CRLCDT2 and CRLDDB2 are two key E3 ubiquitin ligases involved in DNA replication and DNA damage repair.
Xiaojun Wu, Min Yu, Zhuxia Zhang, Feng Leng, Yue Ma, Ni Xie, Fei Lu   +6 more
doaj   +1 more source

Pitfalls and missing links in current understanding of 4D genomes

Quantitative Biology, Volume 14, Issue 2, June 2026.
Abstract The spatial and temporal organization of the genome—collectively termed the 4D genome—is pivotal for regulating gene expression, maintaining genome stability, and guiding development. The convergence of chromosome conformation capture technologies, super‐resolution microscopy, and single‐cell epigenomics has transformed our understanding of ...
Michael Q. Zhang
wiley   +1 more source

Regulation of CDC6, Geminin, and CDT1 in Human Cells that Undergo Polyploidization [PDF]

Molecular Biology of the Cell, 2002
Endomitosis is the process by which mammalian megakaryocytes become polyploid during terminal differentiation. As in other endoreplicating cells, cyclin-cdk complexes are distinctly regulated, probably to overcome the strict mechanisms that prevent rereplication in most somatic cells.
Bermejo, Rodrigo, Vilaboa, Nuria, Calés, Carmela   +2 more
openaire   +3 more sources

DNA damaging chemical-induced Cdt1 degradation.

, 2013
A and B. DNA damage-inducing reagent MMS or zeocin-induced PCNA-CRL4Cdt2 mediated degradation of Cdt1. 293T cells were treated with MMS (0.5 mM) or zeocin (2 mg/ml) for 1 h, and Cdt1 levels and γH2AX levels were examined (A).
Takeshi Maeda (304955), Toshihiro Takami (304953), Yasushi Shiomi (304958), Yoshinori Yasutani (304954), Hideo Nishitani (304959), Hiroki Sakaguchi (304952), Takashi Ishii (304957), Masayuki Morino (304956)   +7 more
core   +1 more source