Results 41 to 50 of about 10,920,537 (245)

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Adenylylation of Gyrase and Topo IV by FicT Toxins Disrupts Bacterial DNA Topology [PDF]

, 2015
Toxin-antitoxin (TA) modules are ubiquitous molecular switches controlling bacterial growth via the release of toxins that inhibit cell proliferation.
Gerdes, K., Glatter, Timo, Dehio, Christoph, Alexander Harms, Goepfert, A., Scheu, P., Kenn Gerdes, Glatter, T. ; https://orcid.org/, Schirmer, T., Harms, Alexander, Christoph Dehio, Harms, A., Timo Glatter, Stanger, Frédéric Valentin, Gerdes, Kenn, Dehio, C., Schirmer, Tilman, Harms, Alexander; id_orcid, de Jong, I., de Jong, Imke Greet, Stanger, F., Tilman Schirmer, Patrick Daniel Scheu, Arnaud Goepfert, Scheu, Patrick Daniel, Frédéric Valentin Stanger, Imke Greet de Jong, Goepfert, Arnaud, Harms A, de Jong IG, Gerdes K, Stanger FV, Dehio C, Glatter T, Schirmer T, Scheu PD, Goepfert A   +36 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Utility of plasma cell‐free DNA in metastatic castration‐resistant prostate cancer

IJU Case Reports, 2020
Introduction Cell‐free DNA is suggested as a prognostic biomarker in metastatic castration‐resistant prostate cancer. However, it remains unknown which parameter of cell‐free DNA is correlated with the progression and prognosis of metastatic castration ...
Itsuto Hamano, Shingo Hatakeyama, Tomoko Hamaya, Kyo Togashi, Teppei Okamoto, Hayato Yamamoto, Tohru Yoneyama, Takahiro Yoneyama, Yasuhiro Hashimoto, Chikara Ohyama   +9 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing.

PLoS Neglected Tropical Diseases, 2020
BACKGROUND:Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp.
Jingkai Ji, Bin Li, Jingzhong Li, Wangmu Danzeng, Jiandong Li, Yanping Zhao, Gezhen Qiangba, Qingda Zhang, Nibu Renzhen, Zhuoga Basang, Changlin Jia, Quzhen Gongsang, Jinmin Ma, Yicong Wang, Fang Chen, Hongcheng Zhou, Huasang, Jiefang Yin, Jiandan Xie, Na Pei, Huimin Cai, Huayan Jiang, Huanming Yang, Jian Wang, Asan, Xiumin Han, Junhua Li, Weijun Chen, Dong Yang   +28 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Epstein-Barr virus DNA in serum as an early prognostic marker in children and adolescents with Hodgkin lymphoma

Blood Advances, 2017
: Assay of cell-free DNA in blood offers an approach to assessment of tumor DNA. We sought to determine whether Epstein-Barr virus (EBV) DNA in cell-free blood is also a good surrogate for the presence of tumor DNA in children with Hodgkin lymphoma, as ...
Jennifer J.G. Welch, Cindy L. Schwartz, Meghan Higman, Lu Chen, Allen Buxton, Jennifer A. Kanakry, Samir B. Kahwash, Robert E. Hutchison, Debra L. Friedman, Richard F. Ambinder   +9 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source