Results 141 to 150 of about 4,735,099 (379)

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Protocol to assess changes in mitochondrial respiration in living cultured cells infected with flaviviruses

STAR Protocols
Summary: Mitochondria are essential organelles involved in energy production, making them prime targets for flaviviruses, such as dengue and Zika viruses, to enhance viral replication.
Viviana Andrea Barragan Torres, Laurent Chatel-Chaix   +1 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Protocol for affinity enrichment of polyclonal autoantibodies from human plasma

STAR Protocols
Summary: Autoantibodies (AAbs) contribute to various immune-mediated diseases and are valuable biomarkers for diagnosis, classification, and disease activity. Here, we present a protocol for the affinity enrichment of AAbs from human plasma samples.
Aws Aljnabi, Kelly A. Hagadorn, Louisa E. Neumann, Mary E. Peterson, Bethany Scott, Kazutoyo Miura, Aissata Ongoiba, Safiatou Doumbo, Didier Doumtabe, Shanping Li, Kassoum Kayentao, Boubacar Traore, Silvia Bolland, Peter D. Crompton, Christine S. Hopp   +14 more
doaj   +1 more source

Cell-based Tau seeding assay v1

, 2021
This protocol details the procedure of cell-based Tau seeding assay.
openaire   +1 more source

Cell-based assays for the detection of MOG antibodies: a comparative study

Journal of Neurology, 2020
M. Gastaldi, S. Scaranzin, S. Jarius, Brigitte Wildeman, E. Zardini, G. Mallucci, Eleonora Rigoni, E. Vegezzi, T. Foiadelli, S. Savasta, P. Banfi, M. Versino, L. Benedetti, G. Novi, M. Mancardi, T. Giacomini, P. Annovazzi, D. Baroncini, D. Ferraro, V. Lampasona, M. Reindl, P. Waters, D. Franciotta   +22 more
semanticscholar   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

cell-based assay

Citation: 'cell-based assay' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14113 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Cell-Based In Vitro Assay Automation: Balancing Technology and Data Reproducibility/Predictability [PDF]

, 2020
Brande Thomas-Fowlkes, Steven Cifelli, Sarah Souza, Richard Visconti, Alice Struck, Adam B. Weinglass, Mary Jo Wildey   +6 more
openalex   +1 more source