Results 151 to 160 of about 1,959,228 (334)
Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions
FEBS Letters, EarlyView.Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf +6 more
wiley +1 more source
Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway. [PDF]
Methods Mol Biol, 2022 Wei Z, Zhao J, Zhang L, Xia M.
europepmc +1 more source
Development of an anti-CD2/CD3/CD28 bead-based T-cell proliferation assay [PDF]
, 2014 Louisa Green
openalex +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique
STAR ProtocolsSummary: Extracellular vesicles (EVs) play a key role in cancer development and cellular homeostasis by transferring the biological cargo to recipient cells. Here, we describe steps for screening EV secretion-related genes by combining a microRNA (miRNA)
Tomofumi Yamamoto +4 more
doaj +1 more source
How to improve the sensitivity and specificity of cell-based assays in detecting autoantibodies in neuroimmune diseases. [PDF]
Ann Transl Med, 2023 Gao F +6 more
europepmc +1 more source
FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
STAR ProtocolsSummary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval +3 more
doaj +1 more source
Cell-Based Assays for Smoothened Ubiquitination and Sumoylation. [PDF]
Methods Mol Biol, 2022 Han Y, Jiang J.
europepmc +1 more source