Results 201 to 210 of about 4,481,323 (329)

Protocol for affinity enrichment of polyclonal autoantibodies from human plasma

STAR Protocols
Summary: Autoantibodies (AAbs) contribute to various immune-mediated diseases and are valuable biomarkers for diagnosis, classification, and disease activity. Here, we present a protocol for the affinity enrichment of AAbs from human plasma samples.
Aws Aljnabi, Kelly A. Hagadorn, Louisa E. Neumann, Mary E. Peterson, Bethany Scott, Kazutoyo Miura, Aissata Ongoiba, Safiatou Doumbo, Didier Doumtabe, Shanping Li, Kassoum Kayentao, Boubacar Traore, Silvia Bolland, Peter D. Crompton, Christine S. Hopp   +14 more
doaj  

Conformation-stabilizing ELISA and cell-based assays reveal patient subgroups targeting three different epitopes of AGO1 antibodies. [PDF]

Front Immunol, 2022
Moritz CP, Do LD, Tholance Y, Vallayer PB, Rogemond V, Joubert B, Ferraud K, La Marca C, Camdessanché JP, Honnorat J, Antoine JC.   +10 more
europepmc   +1 more source

Cell-Adhesion Assays: Fabrication of an E-<SMALL>C</SMALL>adherin Substratum and Isolation of Lateral and Basal Membrane Patches [PDF]

, 2004
Frauke Drees, Amy Reilein, W. James Nelson   +2 more
openalex   +1 more source

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

Molecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, James S. Fraser, Martin Figeac, Alexis B. Cortot, Etienne Rouleau, David Tulasne   +14 more
wiley   +1 more source

Phytochemical Profile and Chemopreventive Properties of Cooked Glutinous Purple Rice Extracts Using Cell-Based Assays and Rat Model. [PDF]

Foods, 2022
Guo H, Chariyakornkul A, Phannasorn W, Mahatheeranont S, Wongpoomchai R.   +4 more
europepmc   +1 more source

Miniaturization of Whole Live Cell-Based GPCR Assays Using Microdispensing and Detection Systems [PDF]

, 2004
Oleg Kornienko, Raul Lacson, Priya Kunapuli, Jonathan E. Schneeweis, Ira Hoffman, Todd Smith, Melissa Alberts, James Inglese, Berta Strulovici   +8 more
openalex   +1 more source

Etoposide‐induced cancer cell death: roles of mitochondrial VDAC1 and calpain, and resistance mechanisms

Molecular Oncology, EarlyView.
The complex mode of action of the topoisomerase II inhibitor etoposide in triggering apoptosis involves several mechanisms: overexpression of the mitochondrial protein VDAC1, leading to its oligomerization and formation of a large channel that mediates the release of pro‐apoptotic protein; and overexpression of the apoptosis regulators p53, Bax, and ...
Aditya Karunanithi Nivedita, Varda Shoshan‐Barmatz   +1 more
wiley   +1 more source

Discovering cell-active BCL6 inhibitors: effectively combining biochemical HTS with multiple biophysical techniques, X-ray crystallography and cell-based assays. [PDF]

Sci Rep, 2022
Pierrat OA, Liu M, Collie GW, Shetty K, Rodrigues MJ, Le Bihan YV, Gunnell EA, McAndrew PC, Stubbs M, Rowlands MG, Yahya N, Shehu E, Talbot R, Pickard L, Bellenie BR, Cheung KJ, Drouin L, Innocenti P, Woodward H, Davis OA, Lloyd MG, Varela A, Huckvale R, Broccatelli F, Carter M, Galiwango D, Hayes A, Raynaud FI, Bryant C, Whittaker S, Rossanese OW, Hoelder S, Burke R, van Montfort RLM.   +33 more
europepmc   +1 more source

Detection of Endonuclease III- and 8-Oxoguanine Glycosylase-sensitive Base Modifications in γ-Irradiated DNA and Cells by the Aldehyde Reactive Probe (ARP) Assay [PDF]

, 2004
Mohammed Mohsin Ali, Satofumi Kurisu, Yoshihiro Yoshioka, Hiroaki Terato, Yoshihiko Ohyama, Kihei Kubo, Hiroshi Ide   +6 more
openalex   +1 more source

Respiratory complex I‐mediated NAD+ regeneration regulates cancer cell proliferation through the transcriptional and translational control of p21Cip1 expression by SIRT3 and SIRT7

Molecular Oncology, EarlyView.
NAD+ regeneration by mitochondrial complex I NADH dehydrogenase is important for cancer cell proliferation. Specifically, NAD+ is necessary for the activities of NAD+‐dependent deacetylases SIRT3 and SIRT7, which suppress the expression of p21Cip1 cyclin‐dependent kinase inhibitor, an antiproliferative molecule, at the translational and transcriptional
Masato Higurashi, Kazunori Mori, Hidetsugu Nakagawa, Momoko Uchida, Fumihiro Ishikawa, Motoko Shibanuma   +5 more
wiley   +1 more source